Team:Pittsburgh/Notebook

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Office of the Provost Department of Bioengineering Department of Biological Sciences Department of Chemistry Swanson School of Engineering
IDT NEB Experiment

Our weekly progress

Contents

Week 1: May 23 - May 27

Wet Lab

  • Training begins
  • Grow Top 10 competent cells.

Dry Lab

  • Brainstorm genetic circuits for a thallium sensor
  • Lab safety training
Week 1 Notebook Back to Top

Week 2: May 31 - June 3

Wet Lab

  • Test efficiency of competent cells
  • Test cell free extract reaction with T7-GFP plasmid

Dry Lab

  • Contact museums and summer programs for outreach opportunities
  • Lab safety training
Week 2 Notebook Back to Top

Week 3: June 6 - June 12

Wet Lab

  • Transform T7 promoter, amilCP, and terminator
  • Begin assembly by ligating linearized T7 promoter and amilCP

Dry Lab

  • Contact museums and summer programs for outreach opportunities
Week 3 Notebook Back to Top

Week 4: June 13 - June 17

Wet Lab

  • Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP
  • Send promising T7 promoter -- amilCP ligations to be sequenced
  • Perform double digest of T7 promoter and terminator from last week
  • Ligate double-digested T7 promoter to new reporters (lacZ and eGFP)

Dry Lab

  • TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set
Week 4 Notebook Back to Top