Team:NCKU Tainan/Safety
How did we address the safety issues in our project?
- A non-pathogenic chassis, i.e. E.coli DH5α, E.coli BL21 (DE3), is chosen.
- The chassis is in small enclosed space as a barrier to prevent contamination.
- "Suicide-gene inducers" in the enclosed space are designed to kill E.coli in the bio-sensing strip.
Our E.coli can sense urea glucose. When the glucose level is high, the bacteria can express red fluorescent protein (RFP) as a reporter to give a signal which can be detected using excitation. After the detection, the “suicide-gene inducers”, arabinose, induce the pBAD promoter (BBa_K206000) and then activate the transcription of Lysis protein (BBa_K117000).
Build a safe lab
We operated the project in the Biosafety Level 1 (BSL-1) Lab. The space is suitable for experiments in all of our chassis which are Risk Group 1 organisms. Additionally, we always handled biological materials in laminar flow hood. All of biowastes were autoclaved at the typical 121 °C/15 psi for 15 minutes.
Obeying the laws and regulations of biosafety in our Country/ University
The Environmental Protection, Safety and Health Center is responsible for the safety of biology labs at Nation Cheng-Kung University (Guidelines for Laboratory Biosafety). And Centers for Disease Control (CDC) is the department to govern biosafety for research laboratories in our country (Laboratory Biosafety Management: A Compilation of Regulations and Administrative Guidance).
Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems? 寄送貨運公司? 注意事項? 是否需要準備申請文件之類的前置作業?
