Proof of the Chemical Transformation of the e-coli
We used the LIC Method for cloning PETase and E-coli. The basic mechanism of cloning is simple: put gene A into plasmid or the target vector to make it work. With restriction enzyme, we cut the plasmid and put gene A into the part where it is cut out. From this on, the plasmid will be called a backbone. To fit in gene A, we have to make the ends of the cut backbone complementary with gene A. The method of annealing single stranded complementary is by using LIC (Ligation Independent Cloning). We use PCR for this process. Then, we mix gene A and the backbone and insert it in e.coli for transformation. This is how we put PETase into the backbone vector. A bacillus is used to put PETase on the surface to expose it to external surroundings. Our experiment has finished putting PETase and backbone in the e.coli, but we are in the process of verifying our results.
