Team:Pittsburgh/Parts

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Office of the Provost Department of Bioengineering Department of Biological Sciences Department of Chemistry Swanson School of Engineering
IDT NEB Experiment

The Parts we used to build our circuit

The Parts Pitt iGEM developed over the summer are listed below. Take a look by following the provided links.




Part Name

Part Description

Part Number
T3 Promoter Promoter for the T3 RNA Polymerase BBa_K2084000
T3 RNA Polymerase Encodes the T3 RNAP BBa_K2084001
pT7 Toehold Regulatory ribosome binding site with T7 RNAP promoter BBa_K2084002
pT7 Regulated T3 RNA Polymerase Composite made up of our pT7-Toehold, T3 RNAP, and a terminator. To be used to control and regulate sequences with a T3 RNAP promoter. BBa_K2084003
pT3 with RBS T3 promoter with an RBS site added BBa_K2084004
pT7 Toehold Regulated LacZ BBa_K2084002 with a mutagenized lacZ BBa_K2084005
pT3 Controlled GFP T3 RNAP promoter site to translate GFP BBa_K2084006
pT7 Controlled T3 RNAP Translated by T7 RNAP to create T3 RNAP BBa_K2084007
T3 RNA Polymerase Amplifier T3 promoter and gene BBa_K2084008
pT3 Controlled LacZ LacZ reporter regulated by T3 RNAP BBa_K2084009