Team:Pittsburgh/HP/Gold
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How we integrated human practices into our project. Take a look at the human practices summary page and the silver medal criterion page for more about human practices.
Contents
Product Design
From our discussion with Dr. Bain, we decided to focus more on lead detection than thallium detection. In the circuit, it was a simple matter of switching the DNAzyme. However, the general population would benefit more from a lead sensor than a thallium sensor. Lead is a much more widespread problem because many houses still use lead pipes, which can corrode in acidic water. The risk lead contamination poses, especially to young children, means that lead detection is more pressing than thallium detection. Although thallium is also very poisonous, the risk of thallium poisoning from water is not as great. We also decided to use a reporter that could be detected with the naked eye. Since LacZ is an enzyme, it is limited by the amount of substrate, not by the amount of protein that is produced in the cell-free reaction. Also, the color change from yellow to purple is obvious, which would decrease the uncertainty in reading the results of the test.
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