Difference between revisions of "Team:LambertGA"

 
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<header>
 
<header>
 
<div id="headerLinks">
 
<div id="headerLinks">
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<img src="https://static.igem.org/mediawiki/2016/9/9e/T--LambertGA--longhorn_iGEM_logo.png" id="logo">
 
 
<a href="https://2016.igem.org/Team:LambertGA" class="dropbtn">Home</a><!--
 
<a href="https://2016.igem.org/Team:LambertGA" class="dropbtn">Home</a><!--
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--><li class="dropdown" >
 +
      <a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a>
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      <div class="dropdown-content">
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Team">Team</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a>
 +
    </div>
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  </li><!--
 
--><li class="dropdown">
 
--><li class="dropdown">
 
   <a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a>
 
   <a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a>
−
     <div class="dropdown-content">
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     <div class="dropdown-content" style="transition: display 0.5s ease-in-out;">
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       <a href="https://2016.igem.org/Team:LambertGA/Description">Description</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Description">Description</a>
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       <a href="https://2016.igem.org/Team:LambertGA/Design">Design</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Design">Design</a>
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       <a href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a>
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       <a href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a>
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       <a href="https://2016.igem.org/Team:LambertGA/Demonstrate">Demonstrate</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Results">Results</a>
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      <a href="https://2016.igem.org/Team:LambertGA/Results">Results</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Notebook">Notebook</a>
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       <a href="https://2016.igem.org/Team:LambertGA/Notebook">Notebook</a>
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     </div>
 
     </div>
 
   </li><!--
 
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       <a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a>
 
       <a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a>
 
       <div class="dropdown-content">
 
       <div class="dropdown-content">
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       <a href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;"  
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      <a href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a>
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       <a href="https://2016.igem.org/Team:LambertGA/Part_Collection">Part Collection</a>
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href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a>
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  </li><!--
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a>
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      <a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a>
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      <a href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a>
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       <a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a>
 
       <a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a>
 
       <div class="dropdown-content">
 
       <div class="dropdown-content">
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       <a href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Human_Practices">Human Practices</a>
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       <a href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a>
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      <a href="https://2016.igem.org/Team:LambertGA/HP/Integrated_Pract">Integrated Practices</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a>
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       <a href="https://2016.igem.org/Team:LambertGA/HP/Engagements">Engagements</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Integrated_Practices">Integrated Practices</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Engagement">Engagement</a>
 
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     </div>
 
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--><li class="dropdown" >
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       <a href="https://2016.igem.org/Team:LambertGA/Awards" class="dropbtn">Awards</a>
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       <a href="https://2016.igem.org/Team:LambertGA/Hardware" class="dropbtn">Awards</a>
 
       <div class="dropdown-content">
 
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       <a href="https://2016.igem.org/Team:LambertGA/Entrepreneurship">Entrepreneurship</a>
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      <a href="https://2016.igem.org/Team:LambertGA/Hardware">Hardware</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Measurement">Measurement</a>
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       <a href="https://2016.igem.org/Team:LambertGA/Software">Software</a>
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Model">Model</a>
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      <a href="https://2016.igem.org/Team:LambertGA/Measurement">Measurement</a>
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             <img src="https://static.igem.org/mediawiki/2016/e/ef/T--LambertGA--clear_banner.jpg" style="width:100%" align="middle">
 
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<center> <h1 id="MainTitle"><b> SWITCH </b></h1>
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<h2> Characterization of Nonlysosomal Proteolysis <h2>
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<h2> Characterization of Nonlysosomal Proteolysis </h2>
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</center>
  
  
 
<div id="banner">
 
<div id="banner">
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<br>
  
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<p style="font-size: 20px;">
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Micronutrient deficiency is one of the leading causes of death in the world. According to the World Health Organization (WHO), more than 2 billion people - over 30% of the world’s population - suffer from micronutrient deficiencies today. In response, healthcare providers around the world have organized research facilities to diagnose and treat micronutrient deficiency. However, diagnosing these deficiencies has proved to be extremely expensive and, in many cases, very time consuming. To address this growing concern, medical researchers and synthetic biologists are creating simple and inexpensive “biosensors” that serve as handy diagnostic tests to use in the field to visualize micronutrient levels and determine whether the patient and population in general are lacking critical micronutrients in their diet. Despite these benefits, this versatile tool often yields inaccurate results, potentially leading to faulty diagnosis and the endangerment of an entire community.
  
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<p style="font-size: 20px;">The concentration of proteins in a cell is determined by both the amount synthesized and the amount degraded. Thus, protein degradation is a crucial aspect of maintaining intramolecular equilibrium. A class of ATPases known as AAA+ Proteins involves a well-known proteolysis mechanism known as ClpXP in which ClpX unfolds and translocates a tagged protein into a sequestered proteolytic compartment in ClpP. 
 
 
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We devised an inducible genetic construct in which ClpXP will degrade a chromoprotein upon induction. The data will be gathered using a device that can quantify the color of the light reflected by the chromoprotein before and after induction. This will ultimately allow us to measure the relative strength of degradation and further characterize a well-known proteolysis mechanism. Our characterization of ClpXP will serve as a precursor for controlled protein delivery in medicines and subsequently a switch for biosensors.
+
With this in mind, the 2016 Lambert iGEM Team has focused primarily on enhancing existing biosensors and aiding in the diagnosis of micronutrient deficiencies throughout the world. We studied protein degradation and devised a “switch” to prevent the overexpression of specific reporters in biosensors in order to fine-tune biosensor accuracy. By using SsrA degradation tags, we characterized ClpXP, a protease involved in non-lysosomal proteolysis in many prokaryotes, to quantify the relative strength of degradation in GFP and purple chromoproteins.
  
 
</p>
 
</p>
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<center>
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<br>
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<img src="https://static.igem.org/mediawiki/2016/e/ef/T--LambertGA--newsilvermedal.jpg">
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<br><br>
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<h2> Silver Medal </h2><br>
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<h2> Nominated for Best Wiki </h2><br>
 +
<h2> Nominated for Best Poster </h2><br>
 +
</center>
  
 
<div class="button_click"  onClick=" parent.location= 'https://2016.igem.org/Special:Upload '">
 
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<a href="http://www.forsyth.k12.ga.us/lhs"><img src="https://static.igem.org/mediawiki/2015/a/a4/Lambert_lhslogo.jpg" class="transparent" style="width:100px;height=130px"></a>
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<a href="http://www.forsyth.k12.ga.us/lhs"><img src="https://static.igem.org/mediawiki/2016/9/9e/T--LambertGA--longhorn_iGEM_logo.png" class="transparent" style="height:100px;padding:20px;"></a>
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<a href="http://www.snapgene.com"> <img src="https://static.igem.org/mediawiki/2015/8/83/Lambert_sglogo.jpg" class="transparent" style="width:200px;height:60px"></a>
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<a href="http://www.foldscope.com/"><img src="https://static.igem.org/mediawiki/2015/9/90/Lambert_foldscopelogo.jpg" class="transparent" style="width:100px;height 150px"></a>  
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<a href="http://www.chick-fil-a.com/"><img src="https://static.igem.org/mediawiki/2015/c/cb/Lambert_cfalogo.jpg" class="transparent" style="width:200px;height:75px"></a>
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<a href="http://atlantasciencefestival.org/"><img src="https://static.igem.org/mediawiki/2015/8/80/Lambert_asflogo.jpg" class="transparent" style="width:150px;height:100px"></a>
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<a href="https://www.neb.com/"><img src="https://static.igem.org/mediawiki/2015/e/e4/Lambert_neblogo.jpg" class="transparent" style="width:200px;height:70px"></a>
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<a href="http://www.nextgenerationfocus.org/"><img src="https://static.igem.org/mediawiki/2015/e/e4/Lambert_ngflogo.jpg" class="transparent" ></a>
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Latest revision as of 20:25, 11 November 2016

Characterization of Nonlysosomal Proteolysis


Micronutrient deficiency is one of the leading causes of death in the world. According to the World Health Organization (WHO), more than 2 billion people - over 30% of the world’s population - suffer from micronutrient deficiencies today. In response, healthcare providers around the world have organized research facilities to diagnose and treat micronutrient deficiency. However, diagnosing these deficiencies has proved to be extremely expensive and, in many cases, very time consuming. To address this growing concern, medical researchers and synthetic biologists are creating simple and inexpensive “biosensors” that serve as handy diagnostic tests to use in the field to visualize micronutrient levels and determine whether the patient and population in general are lacking critical micronutrients in their diet. Despite these benefits, this versatile tool often yields inaccurate results, potentially leading to faulty diagnosis and the endangerment of an entire community.

With this in mind, the 2016 Lambert iGEM Team has focused primarily on enhancing existing biosensors and aiding in the diagnosis of micronutrient deficiencies throughout the world. We studied protein degradation and devised a “switch” to prevent the overexpression of specific reporters in biosensors in order to fine-tune biosensor accuracy. By using SsrA degradation tags, we characterized ClpXP, a protease involved in non-lysosomal proteolysis in many prokaryotes, to quantify the relative strength of degradation in GFP and purple chromoproteins.




Silver Medal


Nominated for Best Wiki


Nominated for Best Poster