Attributions and Acknowledgements

Attributions

Research

Constructs: Arjun Bhatt, Jack Kwon

Measurement Model: Elynna Chang, Noora Chandasir, David Standeven, Jackson Harris

Labwork

Arjun Bhatt, Jack Kwon, Sara Cleland, Julia Leveille, Alyssa Franklin, Lauren Hong, Jackson Harris, Kevin Tao, David Standeven

Proof of Concept: Janet Standeven

Wiki

Design: Neha Balachandran

Design Support: Sara Cleland, Jackson Harris, Arjun Bhatt

Information/Writing: Arjun Bhatt, Jack Kwon, Natalie Shih, Nivi Minjur, Janet Standeven

Pictures/Diagrams: Arjun Bhatt

Design

Spiritwear, Banner, Animations: Elynna Chang

Poster: Alyssa Franklin, Lauren Hong, Julia Leveille, Noora Chandasir

Handouts: Sara Cleland, Lubna Orberger

Outreach

Atlanta Science Festival: Noor Sohal, Elynna Chang, Neha Balachandran

Building with Biology: Nivi Minjur, Noora Chandasir, David Standeven, Natalie Shih

RESA: Noor Sohal, Neha Balachandran

Next Generation Focus: Neha Balachandran

CHD Walk: Noora Chandasir

Sharon Science Day: Sara Cleland

Presentation

Presenters: Arjun Bhatt, Neha Balachandran, Elynna Chang

Script/Other Help: Jack Kwon, Lauren Hong, Jackson Harris

Acknowledgements

General Support

Mark Styczynski: Associate Professor at Georgia Institute of Technology

Monica McNerney: Doctoral Student at Georgia Institute of Technology

Dan Watstein: Doctoral Candidate at Georgia Institute of Technology

2015 Lambert iGEM Seniors: Noor Sohal, Lily Ge, Hanna Minot, Lauren Pan, and Sarah Rupert

Parts Support

Snapgene: Software for sequence analysis

IDT: Synthesis of g blocks

Funding/Materials Support

Lambert High School

National Science Foundation Grant #1254382

Siemens

AGCO

Franklin Technologies

Gofundme

Johns Creek Chick fil A

Rosco: Roscolux

Wiki Support

Joel Joseprabu: Wiki Formatting

Balachandran Srinivasan: Wiki Formatting

Outreach Support

Next Generation Focus/ Grace Chapel Hill Church

Atlanta Science Festival

Atlanta Maker Faire

Sharon Elementary School

Sources

And, S. A. (2009, February 13). Sarita Ahlawat. ClpXP Degrades SsrA-tagged proteins in S.pneumoniae.Retrieved Summer, 2016, from http://jb.asm.org/content/191/8/2894.full

Andersen , J.B. , Sternberg , C. , Poulsen , L.K. , Bjorn , S.P. , Givskov , M. , and Molin , S. ( 1998 ) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria . Appl Environ Microbiol 64 : 2240 – 2246 .

Baker, T. A., & Sauer, R. T. (2011, June 27). ClpXP, an ATP-powered unfolding and protein-degradation machine. Retrieved Summer, 2016, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209554/

Bar-Nun, S., & Glickman, M. H. (2012). Proteasomal AAA-ATPases: Structure and function. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1823(1), 67–82. doi:10.1016/j.bbamcr.2011.07.009. Retrieved Summer, 2016 from http://www.sciencedirect.com/science/article/pii/S0167488911001984

Bohn , C. , Binet , E. , and Bouloc , P. ( 2002 ) Screening for stabilization of proteins with a trans-translation signature in Escherichia coli selects for inactivation of the ClpXP protease . Mol Genet Genomics 266 : 827 –831 .

Burton , R.E. , Siddiqui , S.M. , Kim , Y.I. , Baker , T.A. , and Sauer , R.T. ( 2001 ) Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine . EMBO J 20 : 3092 –3100 .

Ciechanover, A. (2005). Cell death and differentiation - abstract of article: Intracellular protein degradation: From a vague idea thru the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting[ast]. Cell Death & Differentiation, 12(9), 1178–1190. doi:10.1038/sj.cdd.4401692

Cooper, G. M. (2000). Protein degradation. Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/books/NBK9957/

Dhakar, L. (n.d.). Image Color Picker (Z. A., Ed.). Retrieved October 10, 2016, from http://www.colorcodepicker.com/

Farrell, C., Grossman, A., & Sauer, R. (2005). Cytoplasmic degradation of ssrA-tagged proteins.Molecular microbiology., 57(6), 1750–61. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16135238

Flynn , J.M. , Levchenko , I. , Seidel , M. , Wickner , S.H. , Sauer , R.T. , and Baker , T.A. ( 2001 ) Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis . Proc Natl Acad Sci USA 11 : 10584 – 10589.

Georgia Institute of Technology. (2015, September 1). “Bacterial litmus Test” provides inexpensive measurement of Micronutrients. Retrieved from GT News Center, http://www.news.gatech.edu/2015/09/01/bacterial-litmus-test-provides-inexpensive-measurement-micronutrients

Goldberg, A.L., A.S. Menon, S. Goff and D.T. Chin. 1987. The mechanism and regulation of the ATP-dependent protease La from Escherichia coli. Biochem. Soc. Trans. 15: 809-811. Retrieved October 1, 2016 from http://www.fao.org/wairdocs/ilri/x5550e/x5550e0d.htm

Hwang BJ, Woo KM, Goldberg AL, Chung CH. Protease Ti, a new ATP-dependent protease in Escherichia coli,contains protein-activated ATPase and proteolytic functions in distinct subunits. J Biol Chem. 1988;263:8727–8734.

Katayama-Fujimura Y, Gottesman S, Maurizi MR. A multiple-component, ATP-dependent protease from Escherichia coli. J Biol Chem. 1987;262:4477–4485.

Landry, B. P., & Stöckel, J. (2013). Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Applied and Environmental Microbiology,79(8), 2833–2835. doi:10.1128/AEM.03741-12

Léa, D. (2016). Les meilleures banques en ligne - Banque en ligne de l'Université Cefi. Retrieved October 18, 2016, from http://univ-cefi.fr/les-meilleures-banques-en-ligne.html

Lee C, Schwartz MP, Prakash S, Iwakura M, Matouschek A. ATP-Dependent Proteases Degrade Their Substrates by Processively Unraveling Them from the Degradation Signal.

McNerney, M. P., Watstein, D. M., & Styczynski, M. P. (2015). Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems. Metabolic Engineering, 31, 123–131. doi:10.1016/j.ymben.2015.06.011

Minikel, E. V. (2013, June 11). Basics of protein degradation. Retrieved Summer, 2016, from http://www.cureffi.org/2013/07/11/basics-of-protein-degradation/

Mogk A, Schmidt R, Bukau B. The N-end rule pathway for regulated proteolysis: prokaryotic and eukaryotic strategies. Trends Cell Biol. 2007;17:165–172.

Purcell, O., Grierson, C. S., Bernardo, M. di, & Savery, N. J. (2012). Temperature dependence of ssrA-tag mediated protein degradation. Journal of Biological Engineering, 6(1), . doi:10.1186/1754-1611-6-10

Purple color codes. (n.d.). Retrieved October 10, 2016, from http://www.rapidtables.com/web/color/purple-color.htm

RGB Color Gradient Maker. (n.d.). Retrieved October 10, 2016, from http://www.perbang.dk/rgbgradient/

Schrader, E. K., Harstad, K. G., & Matouschek, A. (n.d.). Targeting proteins for degradation. , 5(11), . Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228941/

Snider, J., Thibault, G., & Houry, W. A. (2008). The AAA+ superfamily of functionally diverse proteins. , 9(4), . Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643927/

Tamura, K., Shimada, T., Ono, E., Tanaka, Y., Nagatani, A., Higashi, S., . . . Hara-Nishimura, I. (2003, September). Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants. The Plant Journal, 35(4), 545-555. doi:10.1046/j.1365-313X.2003.01822.x

Tanaka K. The proteasome: overview of structure and functions. Proc Jpn Acad Ser B Phys Biol Sci.2009;85:12–36.

Tao, L., & Biswas, I. (2015). Degradation of SsrA-tagged proteins in streptococci. , 161(Pt 4),. Retrieved September 9, 2016 from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857447/

Tu, D., Lee, J., Ozdere, T., Lee, T. J., & You, L. (2007, January ). Engineering Genetic Circuits: Foundations and Applications. Retrieved from http://people.duke.edu/~you/publications/Tu_etal_SyntheticBiology.pdf

Watstein, D. M., McNerney, M. P., & Styczynski, M. P. (2015). Precise metabolic engineering of carotenoid biosynthesis in Escherichia coli towards a low-cost biosensor. Metabolic Engineering,31, 171–180. doi:10.1016/j.ymben.2015.06.007

Team:LambertGA/Attributions