Team Safety

All team members involved in lab procedures were required to adhere to certain lab guidelines as outlined by our advisors. The lab safety rules include but are not limited to:

  • Wash in, wash out
  • Protect eyes, mucous membranes, open cuts and wounds from contact with biohazard material
  • Do not eat or drink when in the lab area
  • Always use gloves and splash-proof goggles
  • Tie back loose hair
  • Disinfect all surfaces with 70% ethanol prior to working
  • Disinfect all disposable tips, glassware, tubes by soaking in 10% bleach solution for 20 minutes and then disposing in normal waste
  • Dispose of growth plates by disposing into a biohazard container which get autoclaved
  • Check all equipment for good working order, no chips, torn cords, cracks. Report any issues to an instructor immediately
  • When pipetting, don’t touch tip to side of container
  • Don’t lay caps of tubes upside down. Use masking tape to hold to bottom of cabinets
  • Clean work area with 70% ethanol after working
  • Clean up all glassware and labware before leaving lab
  • Place all backpacks and stools to the side of the lab to keep walkways clear
  • Always know the correct procedure for disposal of lab materials

  • Public Safety

    We took certain measures to ensure that our project did not pose a threat to public safety. All E. coli strains handled were non-pathogenic, and are unlikely to survive in the wild even if released. All biomaterials were disposed of properly and all relevant lab equipment was autoclaved after use. Team members were not allowed outside of the lab while wearing gloves and refrained from touching doorknobs or any surfaces that would come in contact with the public. We cleaned all lab surfaces after use to ensure that others did not come in contact with our biomaterials.

    Environmental Safety

    To ensure that our project did not propose a hazard to the environment, we used only transformation strains that were purchased through New England Biolabs or from the CSCG Keio Collection. Our project aim is to further characterize the degradation strength of two degradation tags, DAS and LAA, and as such our cell lines will never leave the lab, they will not pose a threat to the environment because they are non pathogenic and contained in a laboratory environment.

    In The Lab