Difference between revisions of "Team:LambertGA"

 
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<h2> Characterization of Nonlysosomal Proteolysis <h2>
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<h2> Characterization of Nonlysosomal Proteolysis </h2>
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<h2>Tuning Biosensors in a Switch-like Fashion<h2>
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Micronutrient deficiency is one of the leading causes of death in the world. According to the World Health Organization (WHO), more than 2 billion people - over 30% of the world’s population - suffer from micronutrient deficiencies today. In response, healthcare providers around the world have organized research facilities to diagnose and treat micronutrient deficiency. However, diagnosing these deficiencies has proved to be extremely expensive and, in many cases, very time consuming. To address this growing concern, medical researchers and synthetic biologists are creating simple and inexpensive “biosensors” that serve as handy diagnostic tests to use in the field to visualize micronutrient levels and determine whether the patient and population in general are lacking critical micronutrients in their diet. Despite these benefits, this versatile tool often yields inaccurate results, potentially leading to faulty diagnosis and the endangerment of an entire community.
  
<p style="font-size: 20px;">Three million children die every year as a result of micronutrient deficiencies.  The World Health Organization estimates that over 2 billion people worldwide are at risk and the Center for Disease Control estimates the costs of testing to be in the millions of dollars.  Simple to use, inexpensive biosensors are an area of great interest in synthetic biology.  Quite simply they could save millions of lives by providing inexpensive options to address micronutrient deficiencies while saving millions of dollars.  The focus of this year’s Lambert’s iGEM team was to research the issues within the area of biosensors and engineer cost effective solutions for both scientists engineering the biosensors and the end users. ((((NEW PARAGRAPH)
 
Biosensors often rely on the response of selection promoters to turn on or off protein expression.  The concentration of proteins in a cell is determined by both the amount synthesized and the amount degraded. Thus, protein degradation is a crucial aspect of maintaining intramolecular equilibrium. A class of ATPases known as AAA+ Proteins involves a well-known proteolysis mechanism known as ClpXP in which ClpX unfolds and translocates a tagged protein into a sequestered proteolytic compartment in ClpP. 
 
 
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We engineered an inducible  construct in which ClpXP will degrade a chromoprotein upon induction. Our system will first be tested in well characterized using Green Fluorescent Protein to establish baseline data. Chromo-protein expression data will be gathered using a device that can quantify the color of the light reflected by the chromoprotein before and after induction. This will ultimately allow us to measure the relative strength of degradation and further characterize a well-known proteolysis mechanism. Our characterization of ClpXP will serve as a precursor for controlled protein delivery in a switch for biosensors.
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With this in mind, the 2016 Lambert iGEM Team has focused primarily on enhancing existing biosensors and aiding in the diagnosis of micronutrient deficiencies throughout the world. We studied protein degradation and devised a “switch” to prevent the overexpression of specific reporters in biosensors in order to fine-tune biosensor accuracy. By using SsrA degradation tags, we characterized ClpXP, a protease involved in non-lysosomal proteolysis in many prokaryotes, to quantify the relative strength of degradation in GFP and purple chromoproteins.
  
 
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<h2> Silver Medal </h2><br>
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<h2> Nominated for Best Wiki </h2><br>
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<h2> Nominated for Best Poster </h2><br>
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Latest revision as of 20:25, 11 November 2016

Characterization of Nonlysosomal Proteolysis


Micronutrient deficiency is one of the leading causes of death in the world. According to the World Health Organization (WHO), more than 2 billion people - over 30% of the world’s population - suffer from micronutrient deficiencies today. In response, healthcare providers around the world have organized research facilities to diagnose and treat micronutrient deficiency. However, diagnosing these deficiencies has proved to be extremely expensive and, in many cases, very time consuming. To address this growing concern, medical researchers and synthetic biologists are creating simple and inexpensive “biosensors” that serve as handy diagnostic tests to use in the field to visualize micronutrient levels and determine whether the patient and population in general are lacking critical micronutrients in their diet. Despite these benefits, this versatile tool often yields inaccurate results, potentially leading to faulty diagnosis and the endangerment of an entire community.

With this in mind, the 2016 Lambert iGEM Team has focused primarily on enhancing existing biosensors and aiding in the diagnosis of micronutrient deficiencies throughout the world. We studied protein degradation and devised a “switch” to prevent the overexpression of specific reporters in biosensors in order to fine-tune biosensor accuracy. By using SsrA degradation tags, we characterized ClpXP, a protease involved in non-lysosomal proteolysis in many prokaryotes, to quantify the relative strength of degradation in GFP and purple chromoproteins.




Silver Medal


Nominated for Best Wiki


Nominated for Best Poster