Team:Stockholm/Protocols

Project

In Short Our Path Background Our Project Combat Proteins Sortase A Future Medal achievements

Parts

All parts Basic Parts Part Collection

Lab

Experiments Lab book Protocols

Results

Proof of concept Nuc Lys Def Esp Sort A

Team

Members Collaborations Attributions Sponsors Diary

Human Practices

CARD TECH Integrated Engagement

Safety

NiC

Protocols

Below are the protocols we used in this project.

3A Assembly (Digestion and Ligation)

Biofilm assay

Buffer Preparation, Media, Agar, Agarose, TAE buffer

Collecting BioBrick DNA

Colony Picking

Gel Electrophoresis

Gel Purification

Gibson Assembly

Glycerol Stocks

IMAC Purification

Kirby-Bauer test

PCR Amplification using Phusion Polymerase

PCR Amplification using Taq Polymerase

PCR Amplification using Q5 Polymerase

PCR Purification

Plasmid Extraction

Protein Expression

SDS-PAGE

Sonication

Sortase A Conjugation

Testing Competence of Cells

Thermolysin digestion

Transformation

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