Difference between revisions of "Team:NCKU Tainan/Notebook"

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<div class="head">NOTE / Construction</div>

<div class="head2">Notebook - Construction</div>

<div class="title-line" id="sec1"></div>

<p>We got the bacteria with Biobrick from XMU.</p>

<p>Pre-culture: 3 ml of LB (with Chloramphenicol) was inoculated with one colony from LB agar plate.</p>

 

<div class="title-line" id="sec2"></div>

<div class="title-content">Got the glucose sensitive promoter (PpI, Pcar) by PCR</div>

<h5>2016.04.16</h5>

              <i class="glyphicon glyphicon-ok"></i>Result: After gel purified, the concentration of the DNA was too low to do construction.</p>

              <i class="glyphicon glyphicon-ok"></i>Result: Some of the colonies were correct.</p>

            <p>Pre-culture: 3 ml of LB (with Ampicillin) was inoculated with one correct colony from the agar plate.</p>

              <i class="glyphicon glyphicon-ok"></i>Result: The size of pMD19T-PpI and pMD19T-Pcar were correct.</p>

 

            <div class="title-line" id="sec3"></div>

<div class="title-content">Constructed pSB1C3-B0034-K592009</div>

<h5>2016.04.30</h5>

              <i class="glyphicon glyphicon-ok"></i>Result: The size of pSB1C3-B0034-K592009 was correct.

</p>

 

            <div class="title-line" id="sec3"></div>

<div class="title-content">Construct pMD19T-Pcar-B0034-K592009 and pMD19T-PpI-B0034-K592009</div>

 

 

<ul id="sidemenu">

<li><a href="#" onclick="toEvent('section1');">Section 1</a></li>

<li><a href="#" onclick="toEvent('section2');">Section 2</a></li>

</ul>

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