Difference between revisions of "Team:NCKU Tainan/Safety"

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    <meta charset="utf-8"><link rel="shortcut icon" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png" type="image/x-icon"><link rel="icon" type="image/png" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png"><link rel="icon" type="image/x-icon" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png"><meta name="viewport" content="width=device-width, initial-scale=1.0"><meta property="og:title" content="Judging Safety Form - iGEM NCKU"><meta property="og:site_name" content="Judging Safety Form - iGEM NCKU"><meta property="og:description" content=""><title>Judging Safety Form - iGEM NCKU</title><meta http-equiv="Content-Type" content="text/html" charset="utf-8"><meta property="og:image" content=""><meta property="og:image:type" content="image/png"><link rel="stylesheet" href="/Team:NCKU_Tainan/css/frame/T--NCKU_Tainan--bootstrap_min_css?ctype=text/css&amp;action=raw"><link href="/Team:NCKU_Tainan/font/T--NCKU_Tainan--NotoSans_css?ctype=text/css&amp;action=raw" rel="stylesheet" type="text/css"><link rel="stylesheet" href="/Team:NCKU_Tainan/font/T--NCKU_Tainan--font-awesome_min_css?ctype=text/css&amp;action=raw"> <link rel="stylesheet" href="/Team:NCKU_Tainan/css/T--NCKU_Tainan--Layout_css?ctype=text/css&amp;action=raw">
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    <style>@font-face { font-family: 'NotoSansCJKtc-Regular';  src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div id="navbar-container" class="container-fluid">  <div class="navbar-header">      <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false">        <span class="sr-only">Toggle navigation</span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>      </button>      <a class="navbar-brand" href="/Team:NCKU_Tainan">        <h1>NCKU</h1><h4>Tainan</h4>      </a>  </div>  <div id="navbar" class="navbar-collapse collapse">    <ul class="nav navbar-nav">      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Project">Background</a></li>          <li><a href="/Team:NCKU_Tainan/Description">Description</a></li>          <li><a href="/Team:NCKU_Tainan/Results">Results</a></li>          <li><a href="/Team:NCKU_Tainan/Model">Modeling</a></li>          <li><a href="/Team:NCKU_Tainan/Parts">Parts</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu2" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Device</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Hardware">Hardware</a></li>          <li><a href="/Team:NCKU_Tainan/Software">Software</a></li>          <li><a href="/Team:NCKU_Tainan/Demonstrate">Demonstrate</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu3" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Judging</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu3">          <li><a href="/Team:NCKU_Tainan/Medal">Medal</a></li>          <li><a href="/Team:NCKU_Tainan/Safety">Safety</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu4" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Team">Team</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu4">          <li><a href="/Team:NCKU_Tainan/Team">Team</a></li>          <li><a href="/Team:NCKU_Tainan/Attributions">Attributions</a></li>          <li><a href="/Team:NCKU_Tainan/Acknowledgement">Acknowledgement</a></li>          <li><a href="/Team:NCKU_Tainan/Collaborations">Collaborations</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu5" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Human_Practices">Human Practices</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu5">          <li><a href="/Team:NCKU_Tainan/Human_Practices">Overview</a></li>          <li><a href="/Team:NCKU_Tainan/Integrated_Practices">Integrated Practices</a></li>          <li><a href="/Team:NCKU_Tainan/Engagement">Engagement</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu6" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Notebook</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu6">          <li><a href="/Team:NCKU_Tainan/Notebook_Construction">Construction</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Functional_Test">Functional Test</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Device_Design">Device Design</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Protocols">Protocols</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Model">Model</a></li>        </ul>      </li>    </ul>    <ul class="nav navbar-nav navbar-right">    </ul>  </div><!--/.nav-collapse --></div><!--/.container-fluid --></nav><div id="container-big"><div id="iGEMbar"></div><div id="line-left"></div><div id="line-left2"></div><div id="line-right"></div><div id="photo-left"></div></div><!--/.container-big --> <style>
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#photo-left { background-image: url("/wiki/images/d/d6/T--NCKU_Tainan--Safety.png"); }
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<div class="container-fluid" style="margin-top:100px">
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<div class="head">JUDGING / Safety</div>
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<div class="content row">
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<div class="col-md-9">
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<div class="head2">Safety</div>
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<div class="title-line" id="method">Safety Issues</div>
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<h5>How did we address the safety issues in our project?</h5>
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<ul><li><b>Non-pathogenic chassis</b>, i.e. <i>E. coli</i> DH5α, <i>E. coli</i> (DE3), were chosen.</li>
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<li>The chassis is in small <b>enclosed space</b> as a barrier to prevent contamination.</li>
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<li><b>"Suicide-gene inducers"</b> in the enclosed space can induce expression of the suicide gene&nbsp;to kill <i>E. coli</i> in the bio-sensing strip.</li>
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<div class="title-line" id="plan">Safety Plan</div>
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<p>Our  <i>E. coli</i> can sense urea glucose. When the glucose level is high, the bacteria can express red fluorescent proteins (RFP) as a reporter to give a signal which can be detected at the state of excitation. After the detection, the “suicide-gene inducers”, arabinose, induce the P<sub>BAD</sub> promoter (<a href="http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a>) and then activate the transcription of the lysis gene (<a href="http://parts.igem.org/Part:BBa_K117000">BBa_K117000</a>). The functional assays are showed in <a href="/Team:NCKU_Tainan/Results#safety">Bio-Safety System (Project/ Results)</a>.</p>
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<img src="/wiki/images/c/c4/T--NCKU_Tainan--safety5.png">
  
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<div class="title-line" id="lab">Safe Lab</div>
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<h5>Building a safe lab</h5>
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<p>We operated the project in the Biosafety Level 1 (BSL-1) Lab. The space is suitable for experiments in all of our chassis which are Risk Group 1 organisms. Additionally, we always handled biological materials in a laminar flow hood. All of biowastes were autoclaved at the typical 121 °C/15 psi for 15 minutes.</p>
  
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<h5>Obeying the laws and regulations of biosafety in our Country/ University</h5>
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<p>Regarding the Environmental Protection, Safety and Health Center is responsible for the safety of biology labs at National Cheng Kung University (<a href="/wiki/images/6/6c/T--NCKU_Tainan--ref3.pdf" target="_blank">Guidelines for Laboratory Biosafety</a>). And Centers for Disease Control (CDC) is the department to govern biosafety for research laboratories in our country (<a href="/wiki/images/a/a1/T--NCKU_Tainan--ref4.pdf" target="_blank">Laboratory Biosafety Management: A Compilation of Regulations and Administrative Guidance</a>).</p>
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<li><a href="#" onclick="return toEvent('method');">Safety Issues</a></li>
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<li><a href="#" onclick="return toEvent('plan');">Safety Plan</a></li>
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<li><a href="#" onclick="return toEvent('lab');">Safe Lab</a></li>
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 16:11, 19 October 2016

Judging Safety Form - iGEM NCKU

JUDGING / Safety
Safety
Safety Issues
How did we address the safety issues in our project?
  • Non-pathogenic chassis, i.e. E. coli DH5α, E. coli (DE3), were chosen.
  • The chassis is in small enclosed space as a barrier to prevent contamination.
  • "Suicide-gene inducers" in the enclosed space can induce expression of the suicide gene to kill E. coli in the bio-sensing strip.
Safety Plan

Our E. coli can sense urea glucose. When the glucose level is high, the bacteria can express red fluorescent proteins (RFP) as a reporter to give a signal which can be detected at the state of excitation. After the detection, the “suicide-gene inducers”, arabinose, induce the PBAD promoter (BBa_K206000) and then activate the transcription of the lysis gene (BBa_K117000). The functional assays are showed in Bio-Safety System (Project/ Results).

Safe Lab
Building a safe lab

We operated the project in the Biosafety Level 1 (BSL-1) Lab. The space is suitable for experiments in all of our chassis which are Risk Group 1 organisms. Additionally, we always handled biological materials in a laminar flow hood. All of biowastes were autoclaved at the typical 121 °C/15 psi for 15 minutes.

Obeying the laws and regulations of biosafety in our Country/ University

Regarding the Environmental Protection, Safety and Health Center is responsible for the safety of biology labs at National Cheng Kung University (Guidelines for Laboratory Biosafety). And Centers for Disease Control (CDC) is the department to govern biosafety for research laboratories in our country (Laboratory Biosafety Management: A Compilation of Regulations and Administrative Guidance).