Difference between revisions of "Team:LambertGA/Parts"

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{{LambertGA}}
 
 
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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#content {
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<body>
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<header>
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<div id="headerLinks">
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<a href="https://2016.igem.org/Team:LambertGA" class="dropbtn">Home</a><!--
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--><li class="dropdown" >
 +
      <a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a>
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      <div class="dropdown-content">
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Team">Team</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a>
 +
    </div>
 +
  </li><!--
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--><li class="dropdown">
 +
  <a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a>
 +
    <div class="dropdown-content" style="transition: display 0.5s ease-in-out;">
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Description">Description</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Design">Design</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Results">Results</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Notebook">Notebook</a>
 +
    </div>
 +
  </li><!--
 +
--><li class="dropdown" >
 +
      <a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a>
 +
      <div class="dropdown-content">
 +
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Parts">Parts</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a>
 +
    </div>
 +
  </li><!--
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--><a  href="https://2016.igem.org/Team:LambertGA/Attributions"class="dropbtn">Attributions</a><!--
 +
--><a  href="https://2016.igem.org/Team:LambertGA/Safety"class="dropbtn">Safety</a><!--
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--><li class="dropdown" >
 +
      <a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a>
 +
      <div class="dropdown-content">
 +
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Human_Practices">Human Practices</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Integrated_Practices">Integrated Practices</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Engagement">Engagement</a>
 +
    </div>
 +
  </li><!--
 +
--><li class="dropdown" >
 +
      <a href="https://2016.igem.org/Team:LambertGA/Hardware" class="dropbtn">Awards</a>
 +
      <div class="dropdown-content">
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Hardware">Hardware</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Measurement">Measurement</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Model">Model</a>
 +
    </div>
 +
  </li>
  
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
−
</ul>
 
 
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<h5>Part Table </h5>
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<div class="highlight">
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<groupparts>iGEM2016 Example</groupparts>
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<div class="row">
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<div class="col-md-12 col-sm-10">
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<br><br>
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<center> <h1 id="MainTitle"><b> Parts </b></h1>
 +
<img src="https://static.igem.org/mediawiki/2016/2/26/T--LambertGA--purpleline.jpg" style="width:18%; margin:auto;"></center>
 +
<br>
 +
 
 +
<center>
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;" href="http://parts.igem.org/Part:BBa_K1911002" style="text-decoration: none; color: #D49AE6;"> BBa_K1911002: ClpP </a>
 +
 
 +
 
 +
<br><br>
 +
 
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911003" style="text-decoration: none; color: #D49AE6;"> BBa_K1911003: ClpX </a>
 +
 
 +
 
 +
<br><br>
 +
 
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911005" style="text-decoration: none; color: #D49AE6;"> BBa_K1911005: eGFP </a>
 +
 
 +
 
 +
<br><br>
 +
 
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911000" style="text-decoration: none; color: #D49AE6;"> BBa_K1911000: pλr-LacI </a>
 +
 
 +
<br><br>
 +
 
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911001" style="text-decoration: none; color: #D49AE6;"> BBa_K1911001: pLac-ClpX-ClpP-CI </a>
 +
<br><br>
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911004" style="text-decoration: none; color: #D49AE6;"> BBa_K1911004: pλr-LacI-eGFP-LAA-pLac-ClpX-ClpP-CI </a>
 +
 
 +
</center>
 +
<br>
 +
<br>
 +
<br>
 +
</p>
 +
</div></div></div></div>
 +
</center>
 +
</section>
 +
 
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<div class="column full_size" style="width:70%; margin:auto;" >
 +
<div>
 +
Our 2016 iGEM team used existing Biobrick parts from the Uppsala team (BBa_K1033906) and also the Endy Lab (BBa_M0050-LAA, M0052-DAS). We were trying to further Edinburgh’s work by expanding upon their experiments with GFP and the degradation tags. We sought to do this by replacing GFP with the tsPurple chromoprotein and characterizing the ClpXP system. We attempted to characterize these previous parts but ran into some obstacles while working with them.
 +
<br><br>
 +
We first obtained the tsPurple sequence from the 2013 Uppsala iGEM wiki page. We ordered the sequence online from IDT, hydrated it, and proceeded to carry out the 3A assembly procedures to create our constructs. We had the tsPurple with no degradation tag, tsPurple with the LAA degradation tag, and tsPurple with the DAS degradation tag. The degradation tags were obtained from the Endy Lab sequencing and correspond to strong degradation for LAA and moderate for DAS.
 +
<br><br>
 +
We sought to improve upon the LAA and DAS degradation tags by showing their relative strength in degrading the tsPurple chromoprotein and we also sought to improve upon the characterization of the tsPurple gene so that its relative degradation may be present for anyone who needs it.
 
</div>
 
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<br><br>
  
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<div id="sponsors-bottom">
  
 +
<a href="http://www.forsyth.k12.ga.us/lhs"><img src="https://static.igem.org/mediawiki/2016/9/9e/T--LambertGA--longhorn_iGEM_logo.png" class="transparent" style="height:100px;padding:20px;"></a>
 +
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</div>
  
  
 
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</html>

Latest revision as of 20:55, 19 October 2016



Parts


BBa_K1911002: ClpP

BBa_K1911003: ClpX

BBa_K1911005: eGFP

BBa_K1911000: pλr-LacI

BBa_K1911001: pLac-ClpX-ClpP-CI

BBa_K1911004: pλr-LacI-eGFP-LAA-pLac-ClpX-ClpP-CI



Our 2016 iGEM team used existing Biobrick parts from the Uppsala team (BBa_K1033906) and also the Endy Lab (BBa_M0050-LAA, M0052-DAS). We were trying to further Edinburgh’s work by expanding upon their experiments with GFP and the degradation tags. We sought to do this by replacing GFP with the tsPurple chromoprotein and characterizing the ClpXP system. We attempted to characterize these previous parts but ran into some obstacles while working with them.

We first obtained the tsPurple sequence from the 2013 Uppsala iGEM wiki page. We ordered the sequence online from IDT, hydrated it, and proceeded to carry out the 3A assembly procedures to create our constructs. We had the tsPurple with no degradation tag, tsPurple with the LAA degradation tag, and tsPurple with the DAS degradation tag. The degradation tags were obtained from the Endy Lab sequencing and correspond to strong degradation for LAA and moderate for DAS.

We sought to improve upon the LAA and DAS degradation tags by showing their relative strength in degrading the tsPurple chromoprotein and we also sought to improve upon the characterization of the tsPurple gene so that its relative degradation may be present for anyone who needs it.