Difference between revisions of "Team:LambertGA/Parts"

 
(19 intermediate revisions by 3 users not shown)
Line 5: Line 5:
 
#sideMenu { display:none; }
 
#sideMenu { display:none; }
  
 +
a:hover {
 +
text-decoration: none;
 +
}
  
 
#mainContainer{
 
#mainContainer{
Line 18: Line 21:
  
 
body { background-color: white; margin: auto; padding: 0px; width: 100%;}
 
body { background-color: white; margin: auto; padding: 0px; width: 100%;}
 +
 +
a.drplink {
 +
color:white;
 +
}
 +
a.drplink:hover {
 +
color:dimgray;
 +
}
 +
 +
a.partlink {
 +
color:#D49AE6;
 +
}
 +
a.partlink:hover {
 +
color:dimgray;
 +
}
 +
 +
 +
div {
 +
color: gray;
 +
}
  
 
#content {  
 
#content {  
Line 37: Line 59:
 
p {
 
p {
 
margin: auto;
 
margin: auto;
−
width: 50%;
+
width: 75%;
 +
color: gray;
 
}
 
}
  
Line 69: Line 92:
  
 
#MainTitle {
 
#MainTitle {
−
padding: 60px;
+
padding-top: 50px;
−
font-size: 60px; }
+
padding-bottom: 7px;
 +
font-size: 45px;
 +
color: dimgray; }
  
  
Line 85: Line 110:
 
header h1{
 
header h1{
 
text-align:center;
 
text-align:center;
 +
color: dimgray;
 +
}
 +
 +
h2{
 +
color: #D49AE6;
 
}
 
}
  
Line 95: Line 125:
 
height: 50px;
 
height: 50px;
 
line-height: 50px;
 
line-height: 50px;
−
background-color: lavender;
+
background-color: white;
 
margin: auto;
 
margin: auto;
 
text-align:center;
 
text-align:center;
−
opacity: 0.9;
+
opacity: 0.7;
 
}
 
}
  
Line 192: Line 222:
 
   height: 100%;
 
   height: 100%;
 
   width: 100%;
 
   width: 100%;
−
   background-color: lavender;
+
   background-color: white;
 
   color: #D49AE6;
 
   color: #D49AE6;
 
   padding: 14px;
 
   padding: 14px;
Line 214: Line 244:
 
     position: absolute;
 
     position: absolute;
 
     background-color: #D49AE6;
 
     background-color: #D49AE6;
−
     min-width: 140px;
+
     min-width: 200px;
 
     text-align: left;
 
     text-align: left;
 
     box-shadow: 0px 8px 12px 0px rgba(0,0,0,0.2);
 
     box-shadow: 0px 8px 12px 0px rgba(0,0,0,0.2);
Line 263: Line 293:
 
}
 
}
 
</style>
 
</style>
 +
  
  
Line 272: Line 303:
  
 
<body>
 
<body>
 +
 +
  
 
<header>
 
<header>
 
<div id="headerLinks">
 
<div id="headerLinks">
−
<img src="https://static.igem.org/mediawiki/2016/9/9e/T--LambertGA--longhorn_iGEM_logo.png" id="logo">
 
 
<a href="https://2016.igem.org/Team:LambertGA" class="dropbtn">Home</a><!--
 
<a href="https://2016.igem.org/Team:LambertGA" class="dropbtn">Home</a><!--
 +
--><li class="dropdown" >
 +
      <a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a>
 +
      <div class="dropdown-content">
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Team">Team</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a>
 +
    </div>
 +
  </li><!--
 
--><li class="dropdown">
 
--><li class="dropdown">
 
   <a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a>
 
   <a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a>
−
     <div class="dropdown-content">
+
     <div class="dropdown-content" style="transition: display 0.5s ease-in-out;">
−
       <a href="https://2016.igem.org/Team:LambertGA/Description">Description</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Description">Description</a>
−
       <a href="https://2016.igem.org/Team:LambertGA/Design">Design</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Design">Design</a>
−
       <a href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a>
−
       <a href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a>
−
       <a href="https://2016.igem.org/Team:LambertGA/Demonstrate">Demonstrate</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Results">Results</a>
−
      <a href="https://2016.igem.org/Team:LambertGA/Results">Results</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Notebook">Notebook</a>
−
       <a href="https://2016.igem.org/Team:LambertGA/Notebook">Notebook</a>
+
 
     </div>
 
     </div>
 
   </li><!--
 
   </li><!--
Line 292: Line 330:
 
       <a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a>
 
       <a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a>
 
       <div class="dropdown-content">
 
       <div class="dropdown-content">
−
      <a href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a>
+
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Parts">Parts</a>
−
      <a href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a>
−
       <a href="https://2016.igem.org/Team:LambertGA/Part_Collection">Part Collection</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a>
−
    </div>
+
−
  </li><!--
+
−
--><li class="dropdown" >
+
−
      <a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a>
+
−
       <div class="dropdown-content">
+
−
      <a href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a>
+
 
     </div>
 
     </div>
 
   </li><!--
 
   </li><!--
Line 308: Line 340:
 
       <a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a>
 
       <a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a>
 
       <div class="dropdown-content">
 
       <div class="dropdown-content">
−
       <a href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a>
+
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Human_Practices">Human Practices</a>
−
       <a href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a>
−
      <a href="https://2016.igem.org/Team:LambertGA/HP/Integrated_Pract">Integrated Practices</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a>
−
       <a href="https://2016.igem.org/Team:LambertGA/HP/Engagements">Engagements</a>
+
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Integrated_Practices">Integrated Practices</a>
 +
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Engagement">Engagement</a>
 
     </div>
 
     </div>
 
   </li><!--
 
   </li><!--
 
--><li class="dropdown" >
 
--><li class="dropdown" >
−
       <a href="https://2016.igem.org/Team:LambertGA/Awards" class="dropbtn">Awards</a>
+
       <a href="https://2016.igem.org/Team:LambertGA/Hardware" class="dropbtn">Awards</a>
 
       <div class="dropdown-content">
 
       <div class="dropdown-content">
−
       <a href="https://2016.igem.org/Team:LambertGA/Entrepreneurship">Entrepreneurship</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Hardware">Hardware</a>
−
      <a href="https://2016.igem.org/Team:LambertGA/Hardware">Hardware</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Measurement">Measurement</a>
−
       <a href="https://2016.igem.org/Team:LambertGA/Software">Software</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Model">Model</a>
−
      <a href="https://2016.igem.org/Team:LambertGA/Measurement">Measurement</a>
+
−
       <a href="https://2016.igem.org/Team:LambertGA/Model">Model</a>
+
 
     </div>
 
     </div>
 
   </li>
 
   </li>
Line 328: Line 359:
  
 
</header>
 
</header>
 +
  
  
Line 334: Line 366:
 
<div class="row">
 
<div class="row">
 
<div class="col-md-12 col-sm-10">
 
<div class="col-md-12 col-sm-10">
 +
 +
  
 
<br><br>
 
<br><br>
−
<center> <h1 id="MainTitle"><b> Description</b></h1>  
+
<center> <h1 id="MainTitle"><b> Parts </b></h1>
−
BBa_K1911002: ClpP
+
<img src="https://static.igem.org/mediawiki/2016/2/26/T--LambertGA--purpleline.jpg" style="width:18%; margin:auto;"></center>
 +
<br>
 +
 
 +
<center>
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;" href="http://parts.igem.org/Part:BBa_K1911002" style="text-decoration: none; color: #D49AE6;"> BBa_K1911002: ClpP </a>
 +
 
 +
 
 
<br><br>
 
<br><br>
−
BBa_K1911003: ClpX
+
 
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911003" style="text-decoration: none; color: #D49AE6;"> BBa_K1911003: ClpX </a>
 +
 
 +
 
 
<br><br>
 
<br><br>
−
BBa_K1911000: pλr-LacI
+
 
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911005" style="text-decoration: none; color: #D49AE6;"> BBa_K1911005: eGFP </a>
 +
 
 +
 
 
<br><br>
 
<br><br>
−
BBa_K1911001: pLac-ClpX-ClpP-CI
+
 
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911000" style="text-decoration: none; color: #D49AE6;"> BBa_K1911000: pλr-LacI </a>
 +
 
 
<br><br>
 
<br><br>
−
BBa_K1911004: pλr-LacI-eGFP-LAA-pLac-ClpX-ClpP-CI
+
 
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911001" style="text-decoration: none; color: #D49AE6;"> BBa_K1911001: pLac-ClpX-ClpP-CI </a>
 +
<br><br>
 +
<a class="partlink" style="transition: color 0.5s ease-in-out;"href="http://parts.igem.org/Part:BBa_K1911004" style="text-decoration: none; color: #D49AE6;"> BBa_K1911004: pλr-LacI-eGFP-LAA-pLac-ClpX-ClpP-CI </a>
 +
 
 +
</center>
 
<br>
 
<br>
 
<br>
 
<br>
Line 353: Line 406:
 
</center>
 
</center>
 
</section>
 
</section>
 +
 +
<div class="column full_size" style="width:70%; margin:auto;" >
 +
<div>
 +
Our 2016 iGEM team used existing Biobrick parts from the Uppsala team (BBa_K1033906) and also the Endy Lab (BBa_M0050-LAA, M0052-DAS). We were trying to further Edinburgh’s work by expanding upon their experiments with GFP and the degradation tags. We sought to do this by replacing GFP with the tsPurple chromoprotein and characterizing the ClpXP system. We attempted to characterize these previous parts but ran into some obstacles while working with them.
 +
<br><br>
 +
We first obtained the tsPurple sequence from the 2013 Uppsala iGEM wiki page. We ordered the sequence online from IDT, hydrated it, and proceeded to carry out the 3A assembly procedures to create our constructs. We had the tsPurple with no degradation tag, tsPurple with the LAA degradation tag, and tsPurple with the DAS degradation tag. The degradation tags were obtained from the Endy Lab sequencing and correspond to strong degradation for LAA and moderate for DAS.
 +
<br><br>
 +
We sought to improve upon the LAA and DAS degradation tags by showing their relative strength in degrading the tsPurple chromoprotein and we also sought to improve upon the characterization of the tsPurple gene so that its relative degradation may be present for anyone who needs it.
 +
</div>
 +
<br><br>
 +
 +
<div id="sponsors-bottom">
 +
 +
<a href="http://www.forsyth.k12.ga.us/lhs"><img src="https://static.igem.org/mediawiki/2016/9/9e/T--LambertGA--longhorn_iGEM_logo.png" class="transparent" style="height:100px;padding:20px;"></a>
 +
 +
</div>
  
  
 
</html>
 
</html>

Latest revision as of 20:55, 19 October 2016



Parts


BBa_K1911002: ClpP

BBa_K1911003: ClpX

BBa_K1911005: eGFP

BBa_K1911000: pλr-LacI

BBa_K1911001: pLac-ClpX-ClpP-CI

BBa_K1911004: pλr-LacI-eGFP-LAA-pLac-ClpX-ClpP-CI



Our 2016 iGEM team used existing Biobrick parts from the Uppsala team (BBa_K1033906) and also the Endy Lab (BBa_M0050-LAA, M0052-DAS). We were trying to further Edinburgh’s work by expanding upon their experiments with GFP and the degradation tags. We sought to do this by replacing GFP with the tsPurple chromoprotein and characterizing the ClpXP system. We attempted to characterize these previous parts but ran into some obstacles while working with them.

We first obtained the tsPurple sequence from the 2013 Uppsala iGEM wiki page. We ordered the sequence online from IDT, hydrated it, and proceeded to carry out the 3A assembly procedures to create our constructs. We had the tsPurple with no degradation tag, tsPurple with the LAA degradation tag, and tsPurple with the DAS degradation tag. The degradation tags were obtained from the Endy Lab sequencing and correspond to strong degradation for LAA and moderate for DAS.

We sought to improve upon the LAA and DAS degradation tags by showing their relative strength in degrading the tsPurple chromoprotein and we also sought to improve upon the characterization of the tsPurple gene so that its relative degradation may be present for anyone who needs it.