(42 intermediate revisions by 5 users not shown) | |||
Line 21: | Line 21: | ||
#content { | #content { | ||
margin: 0px auto; | margin: 0px auto; | ||
− | background-color: | + | background-color: white; |
width: 100%; | width: 100%; | ||
padding: 0px; | padding: 0px; | ||
Line 37: | Line 37: | ||
p { | p { | ||
margin: auto; | margin: auto; | ||
− | width: | + | width: 75%; |
+ | color: gray; | ||
} | } | ||
Line 46: | Line 47: | ||
a { | a { | ||
opacity: 1.0; | opacity: 1.0; | ||
+ | |||
} | } | ||
Line 51: | Line 53: | ||
color: #D49AE6; | color: #D49AE6; | ||
} | } | ||
+ | |||
+ | a:hover { | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | |||
+ | a.drplink { | ||
+ | color:white; | ||
+ | } | ||
+ | a.drplink:hover { | ||
+ | color:dimgray; | ||
+ | } | ||
+ | |||
a.HeaderLinks { | a.HeaderLinks { | ||
− | height: | + | height: 50px; |
position: center; | position: center; | ||
border: none; | border: none; | ||
Line 69: | Line 83: | ||
#MainTitle { | #MainTitle { | ||
− | padding: | + | padding-top: 85px; |
− | font-size: 60px; } | + | padding-bottom: 0px; |
+ | font-size: 60px; | ||
+ | color: dimgray; } | ||
Line 85: | Line 101: | ||
header h1{ | header h1{ | ||
text-align:center; | text-align:center; | ||
+ | color: dimgray; | ||
+ | } | ||
+ | |||
+ | h2{ | ||
+ | color: #D49AE6; | ||
} | } | ||
Line 93: | Line 114: | ||
color: #b8975e; | color: #b8975e; | ||
width: 100%; | width: 100%; | ||
− | height: | + | height: 50px; |
− | line-height: | + | line-height: 50px; |
background-color: white; | background-color: white; | ||
margin: auto; | margin: auto; | ||
text-align:center; | text-align:center; | ||
− | opacity: 0. | + | opacity: 0.7; |
} | } | ||
Line 124: | Line 145: | ||
#sponsors-bottom{ | #sponsors-bottom{ | ||
− | width: | + | width: 100%; |
− | margin: | + | margin: 0px auto; |
text-align: center; | text-align: center; | ||
display: block; | display: block; | ||
Line 132: | Line 153: | ||
#sponsors-bottom a{ | #sponsors-bottom a{ | ||
+ | margin: auto; | ||
+ | width: 50% | ||
display: inline-block; | display: inline-block; | ||
} | } | ||
Line 192: | Line 215: | ||
background-color: white; | background-color: white; | ||
color: #D49AE6; | color: #D49AE6; | ||
− | padding: | + | padding: 14px; |
font-size: 20px; | font-size: 20px; | ||
text-align: left; | text-align: left; | ||
Line 207: | Line 230: | ||
/* Dropdown Content (Hidden by Default) */ | /* Dropdown Content (Hidden by Default) */ | ||
− | .dropdown-content { | + | div.dropdown-content { |
display: none; | display: none; | ||
+ | opacity: 0.0; | ||
position: absolute; | position: absolute; | ||
background-color: #D49AE6; | background-color: #D49AE6; | ||
− | min-width: | + | min-width: 200px; |
text-align: left; | text-align: left; | ||
box-shadow: 0px 8px 12px 0px rgba(0,0,0,0.2); | box-shadow: 0px 8px 12px 0px rgba(0,0,0,0.2); | ||
Line 217: | Line 241: | ||
/* Links inside the dropdown */ | /* Links inside the dropdown */ | ||
− | .dropdown-content a { | + | div.dropdown-content a { |
color: white; | color: white; | ||
padding: 0px 10px; | padding: 0px 10px; | ||
Line 223: | Line 247: | ||
text-decoration: none; | text-decoration: none; | ||
display: block; | display: block; | ||
+ | transition: opacity 0.5s ease-in-out; | ||
} | } | ||
Line 229: | Line 254: | ||
/* Show the dropdown menu on hover */ | /* Show the dropdown menu on hover */ | ||
− | .dropdown:hover .dropdown-content { | + | .dropdown:hover div.dropdown-content { |
display: block; | display: block; | ||
+ | opacity: 1.0; | ||
} | } | ||
+ | |||
/* Change the background color of the dropdown button when the dropdown content is shown*/ | /* Change the background color of the dropdown button when the dropdown content is shown*/ | ||
Line 246: | Line 273: | ||
display: block; | display: block; | ||
margin: auto;; | margin: auto;; | ||
+ | } | ||
+ | #logo{ | ||
+ | height: 50px; | ||
+ | opacity:0.4; | ||
} | } | ||
</style> | </style> | ||
Line 261: | Line 292: | ||
<div id="headerLinks"> | <div id="headerLinks"> | ||
<a href="https://2016.igem.org/Team:LambertGA" class="dropbtn">Home</a><!-- | <a href="https://2016.igem.org/Team:LambertGA" class="dropbtn">Home</a><!-- | ||
+ | --><li class="dropdown" > | ||
+ | <a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a> | ||
+ | <div class="dropdown-content"> | ||
+ | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Team">Team</a> | ||
+ | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a> | ||
+ | </div> | ||
+ | </li><!-- | ||
--><li class="dropdown"> | --><li class="dropdown"> | ||
<a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a> | <a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a> | ||
− | <div class="dropdown-content"> | + | <div class="dropdown-content" style="transition: display 0.5s ease-in-out;"> |
− | <a href="https://2016.igem.org/Team:LambertGA/Description">Description</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Description">Description</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/Design">Design</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Design">Design</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Results">Results</a> |
+ | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Notebook">Notebook</a> | ||
</div> | </div> | ||
</li><!-- | </li><!-- | ||
Line 274: | Line 313: | ||
<a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a> | <a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
− | <a | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" |
− | + | href="https://2016.igem.org/Team:LambertGA/Parts">Parts</a> | |
− | <a | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" |
− | + | href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a> | |
− | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a> | |
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
</div> | </div> | ||
</li><!-- | </li><!-- | ||
Line 290: | Line 325: | ||
<a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a> | <a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
− | <a href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Human_Practices">Human Practices</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a> |
− | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a> | |
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Integrated_Practices">Integrated Practices</a> |
+ | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Engagement">Engagement</a> | ||
</div> | </div> | ||
</li><!-- | </li><!-- | ||
--><li class="dropdown" > | --><li class="dropdown" > | ||
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a href="https://2016.igem.org/Team:LambertGA/Hardware" class="dropbtn">Awards</a> |
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
− | <a | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Hardware">Hardware</a> |
− | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Measurement">Measurement</a> | |
− | <a | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Model">Model</a> |
− | + | ||
− | <a href="https://2016.igem.org/Team:LambertGA/Model">Model</a> | + | |
</div> | </div> | ||
</li> | </li> | ||
Line 313: | Line 347: | ||
<div id="mainbanner"> | <div id="mainbanner"> | ||
− | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/e/ef/T--LambertGA--clear_banner.jpg" style="width:100%" align="middle"> |
</div> | </div> | ||
− | <div class="column full_size" style="background-color: | + | <div class="column full_size" style="background-color:white" > |
Line 323: | Line 357: | ||
− | <center> < | + | <center> <img src="https://static.igem.org/mediawiki/2016/a/a4/T--LambertGA--gray_switch.png" style="width:45%; padding-top:80px;" align="middle"> |
− | <h2> Characterization of Nonlysosomal Proteolysis <h2> | + | <h2> Characterization of Nonlysosomal Proteolysis </h2> |
− | + | </center> | |
<div id="banner"> | <div id="banner"> | ||
+ | </div> | ||
+ | <br> | ||
+ | <p style="font-size: 20px;"> | ||
+ | Micronutrient deficiency is one of the leading causes of death in the world. According to the World Health Organization (WHO), more than 2 billion people - over 30% of the world’s population - suffer from micronutrient deficiencies today. In response, healthcare providers around the world have organized research facilities to diagnose and treat micronutrient deficiency. However, diagnosing these deficiencies has proved to be extremely expensive and, in many cases, very time consuming. To address this growing concern, medical researchers and synthetic biologists are creating simple and inexpensive “biosensors” that serve as handy diagnostic tests to use in the field to visualize micronutrient levels and determine whether the patient and population in general are lacking critical micronutrients in their diet. Despite these benefits, this versatile tool often yields inaccurate results, potentially leading to faulty diagnosis and the endangerment of an entire community. | ||
+ | <br> | ||
+ | <br> | ||
+ | With this in mind, the 2016 Lambert iGEM Team has focused primarily on enhancing existing biosensors and aiding in the diagnosis of micronutrient deficiencies throughout the world. We studied protein degradation and devised a “switch” to prevent the overexpression of specific reporters in biosensors in order to fine-tune biosensor accuracy. By using SsrA degradation tags, we characterized ClpXP, a protease involved in non-lysosomal proteolysis in many prokaryotes, to quantify the relative strength of degradation in GFP and purple chromoproteins. | ||
+ | </p> | ||
+ | <center> | ||
+ | <br> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/e/ef/T--LambertGA--newsilvermedal.jpg"> | ||
+ | <br><br> | ||
− | + | <h2> Silver Medal </h2><br> | |
− | + | <h2> Nominated for Best Wiki </h2><br> | |
− | + | <h2> Nominated for Best Poster </h2><br> | |
− | + | </center> | |
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
− | < | + | |
<div class="button_click" onClick=" parent.location= 'https://2016.igem.org/Special:Upload '"> | <div class="button_click" onClick=" parent.location= 'https://2016.igem.org/Special:Upload '"> | ||
Line 353: | Line 394: | ||
<div id="space2"> | <div id="space2"> | ||
</div> | </div> | ||
+ | |||
<div id="sponsors-bottom"> | <div id="sponsors-bottom"> | ||
− | <a href="http://www.forsyth.k12.ga.us/lhs"><img src="https://static.igem.org/mediawiki/ | + | <a href="http://www.forsyth.k12.ga.us/lhs"><img src="https://static.igem.org/mediawiki/2016/9/9e/T--LambertGA--longhorn_iGEM_logo.png" class="transparent" style="height:100px;padding:20px;"></a> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> |
Latest revision as of 20:25, 11 November 2016
![](https://static.igem.org/mediawiki/2016/a/a4/T--LambertGA--gray_switch.png)
Characterization of Nonlysosomal Proteolysis
Micronutrient deficiency is one of the leading causes of death in the world. According to the World Health Organization (WHO), more than 2 billion people - over 30% of the world’s population - suffer from micronutrient deficiencies today. In response, healthcare providers around the world have organized research facilities to diagnose and treat micronutrient deficiency. However, diagnosing these deficiencies has proved to be extremely expensive and, in many cases, very time consuming. To address this growing concern, medical researchers and synthetic biologists are creating simple and inexpensive “biosensors” that serve as handy diagnostic tests to use in the field to visualize micronutrient levels and determine whether the patient and population in general are lacking critical micronutrients in their diet. Despite these benefits, this versatile tool often yields inaccurate results, potentially leading to faulty diagnosis and the endangerment of an entire community.
With this in mind, the 2016 Lambert iGEM Team has focused primarily on enhancing existing biosensors and aiding in the diagnosis of micronutrient deficiencies throughout the world. We studied protein degradation and devised a “switch” to prevent the overexpression of specific reporters in biosensors in order to fine-tune biosensor accuracy. By using SsrA degradation tags, we characterized ClpXP, a protease involved in non-lysosomal proteolysis in many prokaryotes, to quantify the relative strength of degradation in GFP and purple chromoproteins.
![](https://static.igem.org/mediawiki/2016/e/ef/T--LambertGA--newsilvermedal.jpg)