Difference between revisions of "Team:LambertGA/Attributions"

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{{LambertGA}}
 
 
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">Attributions bronze criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
 
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. </p>
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<h5> Why is this page needed? </h5>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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<div class="column half_size">
 
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<h5> What should this page have?</h5>
 
  
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.firstHeading{
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<li>General Support</li>
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<li>Project support and advice</li>
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<li>Fundraising help and advice</li>
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<li>Lab support</li>
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<li>Difficult technique support</li>
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<li>Project advisor support</li>
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<li>Wiki support</li>
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<li>Presentation coaching</li>
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<li>Human Practices support</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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</ul>
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<h5> Can we base our project on a previous one? </h5>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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<h5>Inspiration</h5>
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hh3{
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<p>Take a look at what other teams have done:</p>
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<ul>
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font-size: 17px
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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}
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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<body>
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<header>
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<div id="headerLinks">
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<a href="https://2016.igem.org/Team:LambertGA" class="dropbtn">Home</a><!--
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--><li class="dropdown" >
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      <a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a>
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      <div class="dropdown-content">
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Team">Team</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a>
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    </div>
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  </li><!--
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--><li class="dropdown">
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  <a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a>
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    <div class="dropdown-content" style="transition: display 0.5s ease-in-out;">
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Description">Description</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Design">Design</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Results">Results</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Notebook">Notebook</a>
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    </div>
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  </li><!--
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--><li class="dropdown" >
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      <a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a>
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      <div class="dropdown-content">
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Parts">Parts</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a>
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    </div>
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  </li><!--
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--><a href="https://2016.igem.org/Team:LambertGA/Attributions"class="dropbtn">Attributions</a><!--
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--><a  href="https://2016.igem.org/Team:LambertGA/Safety"class="dropbtn">Safety</a><!--
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--><li class="dropdown" >
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      <a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a>
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      <div class="dropdown-content">
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Human_Practices">Human Practices</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a>
 +
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Integrated_Practices">Integrated Practices</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Engagement">Engagement</a>
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    </div>
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  </li><!--
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--><li class="dropdown" >
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      <a href="https://2016.igem.org/Team:LambertGA/Hardware" class="dropbtn">Awards</a>
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      <div class="dropdown-content">
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Hardware">Hardware</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Measurement">Measurement</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Model">Model</a>
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    </div>
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  </li>
  
 
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</header>
  
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<div class="column half_size">
 
  
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<h5>Team training and Project start</h5>
 
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
 
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<ul>
 
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
 
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<li>When did you start this course?</li>
 
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<li>Are the syllabus and course materials freely available online?</li>
 
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<li>When did you start your brainstorming?</li>
 
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<li>When did you start in the lab?</li>
 
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<li>When did you start working on  your project?</li>
 
  
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</ul>
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<div class="column full_size" style="width:75%; margin:auto;" >
  
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<script type="text/javascript"></script>
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<br>
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<center> <h1 id="MainTitle"><b> Attributions and Acknowledgements </b></h1> <img src="https://static.igem.org/mediawiki/2016/2/26/T--LambertGA--purpleline.jpg" style="width:18%; margin:auto;"></center>
 +
 +
<br>
 +
<h3> Attributions </h3>
 +
 +
<h2>Research</h2>
 +
<p> <hh3> <b>  Constructs: </b> Arjun Bhatt, Jack Kwon </hh3> </p>
 +
<p> <hh3> <b> Measurement Model: </b> Elynna Chang, Noora Chandasir, David Standeven, Jackson Harris </hh3></p>
 +
 +
<h2>Labwork </h2>
 +
<p> <hh3> Arjun Bhatt, Jack Kwon, Sara Cleland, Julia Leveille, Alyssa Franklin, Lauren Hong, Jackson Harris, Kevin Tao, David Standeven </hh3></p>
 +
<p> <hh3> <b> Proof of Concept: </b> Janet Standeven </hh3> </p>
 +
 +
<h2> Wiki </h2>
 +
<p><hh3> <b> Design: </b> Neha Balachandran </hh3></p>
 +
<p><hh3> <b> Design Support: </b> Sara Cleland, Jackson Harris, Arjun Bhatt </hh3></p>
 +
<p><hh3> <b> Information/Writing: </b> Arjun Bhatt, Jack Kwon, Natalie Shih, Nivi Minjur, Janet Standeven
 +
<p><hh3> <b> Pictures/Diagrams: </b> Arjun Bhatt
 +
 +
<h2> Design </h2>
 +
<p> <hh3> <b> Spiritwear, Banner, Animations: </b> Elynna Chang  </hh3></p>
 +
<p> <hh3> <b> Poster: </b> Alyssa Franklin, Lauren Hong, Julia Leveille, Noora Chandasir</hh3> </p>
 +
<p> <hh3> <b> Handouts: </b> Sara Cleland, Lubna Orberger </hh3></p>
 +
 +
<h2> Outreach </h2>
 +
<p> <hh3> <b> Atlanta Science Festival: </b> Noor Sohal, Elynna Chang, Neha Balachandran </hh3></p>
 +
<p> <hh3> <b> Building with Biology: </b> Nivi Minjur, Noora Chandasir, David Standeven, Natalie Shih </hh3></p>
 +
<p> <hh3> <b> RESA: </b> Noor Sohal, Neha Balachandran </hh3></p>
 +
<p> <hh3> <b> Next Generation Focus: </b> Neha Balachandran</hh3> </p>
 +
<p> <hh3> <b>CHD Walk: </b>Noora Chandasir</hh3> </p>
 +
<p> <hh3> <b>Sharon Science Day: </b> Sara Cleland</hh3> </p>
 +
 +
<h2>Presentation</h2>
 +
<p> <hh3> <b>Presenters: </b>Arjun Bhatt, Neha Balachandran, Elynna Chang</hh3></p>
 +
<p> <hh3> <b>Script/Other Help: </b>Jack Kwon, Lauren Hong, Jackson Harris</hh3></p>
 +
<br>
 +
 +
<h3> Acknowledgements </h3>
 +
 +
<h2> General Support </h2>
 +
<p><hh3> <b>Mark Styczynski: </b> Associate Professor at Georgia Institute of Technology </hh3></p>
 +
<p> <hh3> <b>Monica McNerney: </b>Doctoral Student at Georgia Institute of Technology</hh3> </p>
 +
<p><hh3> <b>Dan Watstein: </b>Doctoral Candidate at Georgia Institute of Technology </hh3></p>
 +
<p><hh3> <b>2015 Lambert iGEM Seniors: </b> Noor Sohal, Lily Ge, Hanna Minot, Lauren Pan, and Sarah Rupert </hh3></p>
 +
 +
 +
<h2> Parts Support </h2>
 +
<p><hh3> <b>Snapgene: </b>Software for sequence analysis</hh3></p>
 +
<p><hh3> <b>IDT: </b>Synthesis of g blocks</hh3></p>
 +
 +
 +
<h2> Funding/Materials Support </h2>
 +
<p><hh3> Lambert High School</hh3></p>
 +
<p><hh3> National Science Foundation Grant #1254382</hh3> </p>
 +
<p><hh3> Siemens</hh3></p>
 +
<p><hh3> AGCO</hh3></p>
 +
<p><hh3> Franklin Technologies</hh3></p>
 +
<p><hh3> Gofundme</hh3></p>
 +
<p><hh3> Johns Creek Chick fil A</hh3></p>
 +
<p><hh3> Rosco: Roscolux </hh3></p>
 +
 +
<h2> Wiki Support </h2>
 +
<p><hh3> Joel Joseprabu: Wiki Formatting</hh3> </p>
 +
<p><hh3> Balachandran Srinivasan: Wiki Formatting</hh3> </p>
 +
 +
<h2> Outreach Support </h2>
 +
<p> <hh3> Next Generation Focus/ Grace Chapel Hill Church</hh3></p>
 +
<p> <hh3> Atlanta Science Festival</hh3></p>
 +
<p> <hh3> Atlanta Maker Faire </hh3></p>
 +
<p> <hh3> Sharon Elementary School </hh3></p>
 +
 +
 +
<h2> Sources </h2>
 +
<p>And, S. A. (2009, February 13). Sarita Ahlawat. ClpXP Degrades SsrA-tagged proteins in S.pneumoniae.Retrieved Summer, 2016, from http://jb.asm.org/content/191/8/2894.full
 +
<br><br>
 +
Andersen , J.B. , Sternberg , C. , Poulsen , L.K. , Bjorn , S.P. , Givskov , M. , and Molin , S. ( 1998 ) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria . Appl Environ Microbiol 64 : 2240 – 2246 .
 +
<br><br>
 +
Baker, T. A., & Sauer, R. T. (2011, June 27). ClpXP, an ATP-powered unfolding and protein-degradation machine. Retrieved Summer, 2016, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209554/
 +
<br><br>
 +
Bar-Nun, S., & Glickman, M. H. (2012). Proteasomal AAA-ATPases: Structure and function. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1823(1), 67–82. doi:10.1016/j.bbamcr.2011.07.009. Retrieved Summer, 2016 from http://www.sciencedirect.com/science/article/pii/S0167488911001984
 +
<br><br>
 +
Bohn , C. , Binet , E. , and Bouloc , P. ( 2002 ) Screening for stabilization of proteins with a trans-translation signature in Escherichia coli selects for inactivation of the ClpXP protease . Mol Genet Genomics 266 : 827 –831 .
 +
<br><br>
 +
Burton , R.E. , Siddiqui , S.M. , Kim , Y.I. , Baker , T.A. , and Sauer , R.T. ( 2001 ) Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine . EMBO J 20 : 3092 –3100 .
 +
<br><br>
 +
Ciechanover, A. (2005). Cell death and differentiation - abstract of article: Intracellular protein degradation: From a vague idea thru the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting[ast]. Cell Death & Differentiation, 12(9), 1178–1190. doi:10.1038/sj.cdd.4401692
 +
<br><br>
 +
Cooper, G. M. (2000). Protein degradation. Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/books/NBK9957/
 +
<br><br>
 +
Dhakar, L. (n.d.). Image Color Picker (Z. A., Ed.). Retrieved October 10, 2016, from http://www.colorcodepicker.com/
 +
<br><br>
 +
Farrell, C., Grossman, A., & Sauer, R. (2005). Cytoplasmic degradation of ssrA-tagged proteins.Molecular microbiology., 57(6), 1750–61. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16135238
 +
<br><br>
 +
Flynn , J.M. , Levchenko , I. , Seidel , M. , Wickner , S.H. , Sauer , R.T. , and Baker , T.A. ( 2001 ) Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis . Proc Natl Acad Sci USA 11 : 10584 – 10589.
 +
<br><br>
 +
Georgia Institute of Technology. (2015, September 1). “Bacterial litmus Test” provides inexpensive measurement of Micronutrients. Retrieved from GT News Center, http://www.news.gatech.edu/2015/09/01/bacterial-litmus-test-provides-inexpensive-measurement-micronutrients
 +
<br><br>
 +
Goldberg, A.L., A.S. Menon, S. Goff and D.T. Chin. 1987. The mechanism and regulation of the ATP-dependent protease La from Escherichia coli. Biochem. Soc. Trans. 15: 809-811. Retrieved October 1, 2016 from http://www.fao.org/wairdocs/ilri/x5550e/x5550e0d.htm
 +
<br><br>
 +
Hwang BJ, Woo KM, Goldberg AL, Chung CH. Protease Ti, a new ATP-dependent protease in Escherichia coli,contains protein-activated ATPase and proteolytic functions in distinct subunits. J Biol Chem. 1988;263:8727–8734.
 +
<br><br>
 +
Katayama-Fujimura Y, Gottesman S, Maurizi MR. A multiple-component, ATP-dependent protease from Escherichia coli. J Biol Chem. 1987;262:4477–4485.
 +
<br><br>
 +
Landry, B. P., & Stöckel, J. (2013). Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Applied and Environmental Microbiology,79(8), 2833–2835. doi:10.1128/AEM.03741-12
 +
<br><br>
 +
Léa, D. (2016). Les meilleures banques en ligne - Banque en ligne de l'Université Cefi. Retrieved October 18, 2016, from http://univ-cefi.fr/les-meilleures-banques-en-ligne.html
 +
<br><br>
 +
Lee C, Schwartz MP, Prakash S, Iwakura M, Matouschek A. ATP-Dependent Proteases Degrade Their Substrates by Processively Unraveling Them from the Degradation Signal.
 +
<br><br>
 +
McNerney, M. P., Watstein, D. M., & Styczynski, M. P. (2015). Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems. Metabolic Engineering, 31, 123–131. doi:10.1016/j.ymben.2015.06.011
 +
<br><br>
 +
Minikel, E. V. (2013, June 11). Basics of protein degradation. Retrieved Summer, 2016, from http://www.cureffi.org/2013/07/11/basics-of-protein-degradation/
 +
<br><br>
 +
Mogk A, Schmidt R, Bukau B. The N-end rule pathway for regulated proteolysis: prokaryotic and eukaryotic strategies. Trends Cell Biol. 2007;17:165–172.
 +
<br><br>
 +
Purcell, O., Grierson, C. S., Bernardo, M. di, & Savery, N. J. (2012). Temperature dependence of ssrA-tag mediated protein degradation. Journal of Biological Engineering, 6(1), . doi:10.1186/1754-1611-6-10
 +
<br><br>
 +
Purple color codes. (n.d.). Retrieved October 10, 2016, from http://www.rapidtables.com/web/color/purple-color.htm
 +
<br><br>
 +
RGB Color Gradient Maker. (n.d.). Retrieved October 10, 2016, from http://www.perbang.dk/rgbgradient/
 +
<br><br>
 +
Schrader, E. K., Harstad, K. G., & Matouschek, A. (n.d.). Targeting proteins for degradation. , 5(11), . Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228941/
 +
<br><br>
 +
Snider, J., Thibault, G., & Houry, W. A. (2008). The AAA+ superfamily of functionally diverse proteins. , 9(4), . Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643927/
 +
<br><br>
 +
Tamura, K., Shimada, T., Ono, E., Tanaka, Y., Nagatani, A., Higashi, S., . . . Hara-Nishimura, I. (2003, September). Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants. The Plant Journal, 35(4), 545-555. doi:10.1046/j.1365-313X.2003.01822.x
 +
<br><br>
 +
Tanaka K. The proteasome: overview of structure and functions. Proc Jpn Acad Ser B Phys Biol Sci.2009;85:12–36.
 +
<br><br>
 +
Tao, L., & Biswas, I. (2015). Degradation of SsrA-tagged proteins in streptococci. , 161(Pt 4),. Retrieved September 9, 2016 from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857447/
 +
<br><br>
 +
Tu, D., Lee, J., Ozdere, T., Lee, T. J., & You, L. (2007, January ). Engineering Genetic Circuits: Foundations and Applications. Retrieved from http://people.duke.edu/~you/publications/Tu_etal_SyntheticBiology.pdf
 +
<br><br>
 +
Watstein, D. M., McNerney, M. P., & Styczynski, M. P. (2015). Precise metabolic engineering of carotenoid biosynthesis in Escherichia coli towards a low-cost biosensor. Metabolic Engineering,31, 171–180. doi:10.1016/j.ymben.2015.06.007
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<br>
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Latest revision as of 20:25, 24 December 2016


Attributions and Acknowledgements


Attributions

Research

Constructs: Arjun Bhatt, Jack Kwon

Measurement Model: Elynna Chang, Noora Chandasir, David Standeven, Jackson Harris

Labwork

Arjun Bhatt, Jack Kwon, Sara Cleland, Julia Leveille, Alyssa Franklin, Lauren Hong, Jackson Harris, Kevin Tao, David Standeven

Proof of Concept: Janet Standeven

Wiki

Design: Neha Balachandran

Design Support: Sara Cleland, Jackson Harris, Arjun Bhatt

Information/Writing: Arjun Bhatt, Jack Kwon, Natalie Shih, Nivi Minjur, Janet Standeven

Pictures/Diagrams: Arjun Bhatt

Design

Spiritwear, Banner, Animations: Elynna Chang

Poster: Alyssa Franklin, Lauren Hong, Julia Leveille, Noora Chandasir

Handouts: Sara Cleland, Lubna Orberger

Outreach

Atlanta Science Festival: Noor Sohal, Elynna Chang, Neha Balachandran

Building with Biology: Nivi Minjur, Noora Chandasir, David Standeven, Natalie Shih

RESA: Noor Sohal, Neha Balachandran

Next Generation Focus: Neha Balachandran

CHD Walk: Noora Chandasir

Sharon Science Day: Sara Cleland

Presentation

Presenters: Arjun Bhatt, Neha Balachandran, Elynna Chang

Script/Other Help: Jack Kwon, Lauren Hong, Jackson Harris


Acknowledgements

General Support

Mark Styczynski: Associate Professor at Georgia Institute of Technology

Monica McNerney: Doctoral Student at Georgia Institute of Technology

Dan Watstein: Doctoral Candidate at Georgia Institute of Technology

2015 Lambert iGEM Seniors: Noor Sohal, Lily Ge, Hanna Minot, Lauren Pan, and Sarah Rupert

Parts Support

Snapgene: Software for sequence analysis

IDT: Synthesis of g blocks

Funding/Materials Support

Lambert High School

National Science Foundation Grant #1254382

Siemens

AGCO

Franklin Technologies

Gofundme

Johns Creek Chick fil A

Rosco: Roscolux

Wiki Support

Joel Joseprabu: Wiki Formatting

Balachandran Srinivasan: Wiki Formatting

Outreach Support

Next Generation Focus/ Grace Chapel Hill Church

Atlanta Science Festival

Atlanta Maker Faire

Sharon Elementary School

Sources

And, S. A. (2009, February 13). Sarita Ahlawat. ClpXP Degrades SsrA-tagged proteins in S.pneumoniae.Retrieved Summer, 2016, from http://jb.asm.org/content/191/8/2894.full

Andersen , J.B. , Sternberg , C. , Poulsen , L.K. , Bjorn , S.P. , Givskov , M. , and Molin , S. ( 1998 ) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria . Appl Environ Microbiol 64 : 2240 – 2246 .

Baker, T. A., & Sauer, R. T. (2011, June 27). ClpXP, an ATP-powered unfolding and protein-degradation machine. Retrieved Summer, 2016, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209554/

Bar-Nun, S., & Glickman, M. H. (2012). Proteasomal AAA-ATPases: Structure and function. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1823(1), 67–82. doi:10.1016/j.bbamcr.2011.07.009. Retrieved Summer, 2016 from http://www.sciencedirect.com/science/article/pii/S0167488911001984

Bohn , C. , Binet , E. , and Bouloc , P. ( 2002 ) Screening for stabilization of proteins with a trans-translation signature in Escherichia coli selects for inactivation of the ClpXP protease . Mol Genet Genomics 266 : 827 –831 .

Burton , R.E. , Siddiqui , S.M. , Kim , Y.I. , Baker , T.A. , and Sauer , R.T. ( 2001 ) Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine . EMBO J 20 : 3092 –3100 .

Ciechanover, A. (2005). Cell death and differentiation - abstract of article: Intracellular protein degradation: From a vague idea thru the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting[ast]. Cell Death & Differentiation, 12(9), 1178–1190. doi:10.1038/sj.cdd.4401692

Cooper, G. M. (2000). Protein degradation. Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/books/NBK9957/

Dhakar, L. (n.d.). Image Color Picker (Z. A., Ed.). Retrieved October 10, 2016, from http://www.colorcodepicker.com/

Farrell, C., Grossman, A., & Sauer, R. (2005). Cytoplasmic degradation of ssrA-tagged proteins.Molecular microbiology., 57(6), 1750–61. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16135238

Flynn , J.M. , Levchenko , I. , Seidel , M. , Wickner , S.H. , Sauer , R.T. , and Baker , T.A. ( 2001 ) Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis . Proc Natl Acad Sci USA 11 : 10584 – 10589.

Georgia Institute of Technology. (2015, September 1). “Bacterial litmus Test” provides inexpensive measurement of Micronutrients. Retrieved from GT News Center, http://www.news.gatech.edu/2015/09/01/bacterial-litmus-test-provides-inexpensive-measurement-micronutrients

Goldberg, A.L., A.S. Menon, S. Goff and D.T. Chin. 1987. The mechanism and regulation of the ATP-dependent protease La from Escherichia coli. Biochem. Soc. Trans. 15: 809-811. Retrieved October 1, 2016 from http://www.fao.org/wairdocs/ilri/x5550e/x5550e0d.htm

Hwang BJ, Woo KM, Goldberg AL, Chung CH. Protease Ti, a new ATP-dependent protease in Escherichia coli,contains protein-activated ATPase and proteolytic functions in distinct subunits. J Biol Chem. 1988;263:8727–8734.

Katayama-Fujimura Y, Gottesman S, Maurizi MR. A multiple-component, ATP-dependent protease from Escherichia coli. J Biol Chem. 1987;262:4477–4485.

Landry, B. P., & Stöckel, J. (2013). Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Applied and Environmental Microbiology,79(8), 2833–2835. doi:10.1128/AEM.03741-12

Léa, D. (2016). Les meilleures banques en ligne - Banque en ligne de l'Université Cefi. Retrieved October 18, 2016, from http://univ-cefi.fr/les-meilleures-banques-en-ligne.html

Lee C, Schwartz MP, Prakash S, Iwakura M, Matouschek A. ATP-Dependent Proteases Degrade Their Substrates by Processively Unraveling Them from the Degradation Signal.

McNerney, M. P., Watstein, D. M., & Styczynski, M. P. (2015). Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems. Metabolic Engineering, 31, 123–131. doi:10.1016/j.ymben.2015.06.011

Minikel, E. V. (2013, June 11). Basics of protein degradation. Retrieved Summer, 2016, from http://www.cureffi.org/2013/07/11/basics-of-protein-degradation/

Mogk A, Schmidt R, Bukau B. The N-end rule pathway for regulated proteolysis: prokaryotic and eukaryotic strategies. Trends Cell Biol. 2007;17:165–172.

Purcell, O., Grierson, C. S., Bernardo, M. di, & Savery, N. J. (2012). Temperature dependence of ssrA-tag mediated protein degradation. Journal of Biological Engineering, 6(1), . doi:10.1186/1754-1611-6-10

Purple color codes. (n.d.). Retrieved October 10, 2016, from http://www.rapidtables.com/web/color/purple-color.htm

RGB Color Gradient Maker. (n.d.). Retrieved October 10, 2016, from http://www.perbang.dk/rgbgradient/

Schrader, E. K., Harstad, K. G., & Matouschek, A. (n.d.). Targeting proteins for degradation. , 5(11), . Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228941/

Snider, J., Thibault, G., & Houry, W. A. (2008). The AAA+ superfamily of functionally diverse proteins. , 9(4), . Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643927/

Tamura, K., Shimada, T., Ono, E., Tanaka, Y., Nagatani, A., Higashi, S., . . . Hara-Nishimura, I. (2003, September). Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants. The Plant Journal, 35(4), 545-555. doi:10.1046/j.1365-313X.2003.01822.x

Tanaka K. The proteasome: overview of structure and functions. Proc Jpn Acad Ser B Phys Biol Sci.2009;85:12–36.

Tao, L., & Biswas, I. (2015). Degradation of SsrA-tagged proteins in streptococci. , 161(Pt 4),. Retrieved September 9, 2016 from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857447/

Tu, D., Lee, J., Ozdere, T., Lee, T. J., & You, L. (2007, January ). Engineering Genetic Circuits: Foundations and Applications. Retrieved from http://people.duke.edu/~you/publications/Tu_etal_SyntheticBiology.pdf

Watstein, D. M., McNerney, M. P., & Styczynski, M. P. (2015). Precise metabolic engineering of carotenoid biosynthesis in Escherichia coli towards a low-cost biosensor. Metabolic Engineering,31, 171–180. doi:10.1016/j.ymben.2015.06.007

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