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background-color: white; | background-color: white; | ||
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body{ | body{ | ||
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header h1{ | header h1{ | ||
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text-align:center; | text-align:center; | ||
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.header{ | .header{ | ||
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margin: 0 auto; | margin: 0 auto; | ||
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width: 900px; | width: 900px; | ||
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#space{ | #space{ | ||
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#sponsors-bottom{ | #sponsors-bottom{ | ||
− | width: | + | width: 100%; |
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text-align: center; | text-align: center; | ||
display: block; | display: block; | ||
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#sponsors-bottom a{ | #sponsors-bottom a{ | ||
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.dropdown:hover .dropdown-content { | .dropdown:hover .dropdown-content { | ||
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− | . | + | .dropbtn:hover { |
background-color: #D49AE6; | background-color: #D49AE6; | ||
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</style> | </style> | ||
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<header> | <header> | ||
<div id="headerLinks"> | <div id="headerLinks"> | ||
− | + | <a href="https://2016.igem.org/Team:LambertGA" class="dropbtn">Home</a><!-- | |
− | <a href="https://2016.igem.org/Team:LambertGA" class="dropbtn"> | + | --><li class="dropdown" > |
− | < | + | <a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a> |
− | </ | + | <div class="dropdown-content"> |
+ | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Team">Team</a> | ||
+ | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a> | ||
+ | </div> | ||
+ | </li><!-- | ||
+ | --><li class="dropdown"> | ||
<a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a> | <a href="https://2016.igem.org/Team:LambertGA/Description" class="dropbtn">Project</a> | ||
− | <div class="dropdown-content"> | + | <div class="dropdown-content" style="transition: display 0.5s ease-in-out;"> |
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Description">Description</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Design">Design</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Experiments">Experiments</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Proof">Proof of Concept</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Results">Results</a> |
+ | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Notebook">Notebook</a> | ||
</div> | </div> | ||
− | </li> | + | </li><!-- |
− | <!-- | + | --><li class="dropdown" > |
− | + | ||
<a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a> | <a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Parts">Parts</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a> |
</div> | </div> | ||
− | </li> | + | </li><!-- |
− | <!-- | + | --><a href="https://2016.igem.org/Team:LambertGA/Attributions"class="dropbtn">Attributions</a><!-- |
− | + | --><a href="https://2016.igem.org/Team:LambertGA/Safety"class="dropbtn">Safety</a><!-- | |
− | + | --><li class="dropdown" > | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | <a href="https://2016.igem.org/Team:LambertGA/Safety"class="dropbtn">Safety</a> | + | |
− | <!-- | + | |
− | + | ||
<a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a> | <a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
− | <a href="https://2016.igem.org/Team:LambertGA/Silver">Silver</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Human_Practices">Human Practices</a> |
− | <a href="https://2016.igem.org/Team:LambertGA/Gold">Gold</a> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a> |
− | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a> | |
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Integrated_Practices">Integrated Practices</a> |
+ | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Engagement">Engagement</a> | ||
</div> | </div> | ||
− | </li> | + | </li><!-- |
− | + | --><li class="dropdown" > | |
− | <a href="https://2016.igem.org/Team:LambertGA/ | + | <a href="https://2016.igem.org/Team:LambertGA/Hardware" class="dropbtn">Awards</a> |
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
− | <a | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Hardware">Hardware</a> |
− | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Measurement">Measurement</a> | |
− | <a | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Model">Model</a> |
− | + | ||
− | <a href="https://2016.igem.org/Team:LambertGA/Model">Model</a> | + | |
</div> | </div> | ||
</li> | </li> | ||
− | |||
</div> | </div> | ||
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− | <div class="column full_size" style=" | + | |
+ | <div class="column full_size" style="width:75%; margin:auto;" > | ||
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− | <center> <h1 id="MainTitle"><b> Attributions </b></h1> </center> | + | <br> |
+ | <center> <h1 id="MainTitle"><b> Attributions and Acknowledgements </b></h1> <img src="https://static.igem.org/mediawiki/2016/2/26/T--LambertGA--purpleline.jpg" style="width:18%; margin:auto;"></center> | ||
+ | |||
+ | <br> | ||
+ | <h3> Attributions </h3> | ||
+ | |||
+ | <h2>Research</h2> | ||
+ | <p> <hh3> <b> Constructs: </b> Arjun Bhatt, Jack Kwon </hh3> </p> | ||
+ | <p> <hh3> <b> Measurement Model: </b> Elynna Chang, Noora Chandasir, David Standeven, Jackson Harris </hh3></p> | ||
+ | |||
+ | <h2>Labwork </h2> | ||
+ | <p> <hh3> Arjun Bhatt, Jack Kwon, Sara Cleland, Julia Leveille, Alyssa Franklin, Lauren Hong, Jackson Harris, Kevin Tao, David Standeven </hh3></p> | ||
+ | <p> <hh3> <b> Proof of Concept: </b> Janet Standeven </hh3> </p> | ||
+ | |||
+ | <h2> Wiki </h2> | ||
+ | <p><hh3> <b> Design: </b> Neha Balachandran </hh3></p> | ||
+ | <p><hh3> <b> Design Support: </b> Sara Cleland, Jackson Harris, Arjun Bhatt </hh3></p> | ||
+ | <p><hh3> <b> Information/Writing: </b> Arjun Bhatt, Jack Kwon, Natalie Shih, Nivi Minjur, Janet Standeven | ||
+ | <p><hh3> <b> Pictures/Diagrams: </b> Arjun Bhatt | ||
+ | |||
+ | <h2> Design </h2> | ||
+ | <p> <hh3> <b> Spiritwear, Banner, Animations: </b> Elynna Chang </hh3></p> | ||
+ | <p> <hh3> <b> Poster: </b> Alyssa Franklin, Lauren Hong, Julia Leveille, Noora Chandasir</hh3> </p> | ||
+ | <p> <hh3> <b> Handouts: </b> Sara Cleland, Lubna Orberger </hh3></p> | ||
+ | |||
+ | <h2> Outreach </h2> | ||
+ | <p> <hh3> <b> Atlanta Science Festival: </b> Noor Sohal, Elynna Chang, Neha Balachandran </hh3></p> | ||
+ | <p> <hh3> <b> Building with Biology: </b> Nivi Minjur, Noora Chandasir, David Standeven, Natalie Shih </hh3></p> | ||
+ | <p> <hh3> <b> RESA: </b> Noor Sohal, Neha Balachandran </hh3></p> | ||
+ | <p> <hh3> <b> Next Generation Focus: </b> Neha Balachandran</hh3> </p> | ||
+ | <p> <hh3> <b>CHD Walk: </b>Noora Chandasir</hh3> </p> | ||
+ | <p> <hh3> <b>Sharon Science Day: </b> Sara Cleland</hh3> </p> | ||
+ | |||
+ | <h2>Presentation</h2> | ||
+ | <p> <hh3> <b>Presenters: </b>Arjun Bhatt, Neha Balachandran, Elynna Chang</hh3></p> | ||
+ | <p> <hh3> <b>Script/Other Help: </b>Jack Kwon, Lauren Hong, Jackson Harris</hh3></p> | ||
+ | <br> | ||
+ | |||
+ | <h3> Acknowledgements </h3> | ||
+ | |||
+ | <h2> General Support </h2> | ||
+ | <p><hh3> <b>Mark Styczynski: </b> Associate Professor at Georgia Institute of Technology </hh3></p> | ||
+ | <p> <hh3> <b>Monica McNerney: </b>Doctoral Student at Georgia Institute of Technology</hh3> </p> | ||
+ | <p><hh3> <b>Dan Watstein: </b>Doctoral Candidate at Georgia Institute of Technology </hh3></p> | ||
+ | <p><hh3> <b>2015 Lambert iGEM Seniors: </b> Noor Sohal, Lily Ge, Hanna Minot, Lauren Pan, and Sarah Rupert </hh3></p> | ||
+ | |||
+ | |||
+ | <h2> Parts Support </h2> | ||
+ | <p><hh3> <b>Snapgene: </b>Software for sequence analysis</hh3></p> | ||
+ | <p><hh3> <b>IDT: </b>Synthesis of g blocks</hh3></p> | ||
+ | |||
+ | |||
+ | <h2> Funding/Materials Support </h2> | ||
+ | <p><hh3> Lambert High School</hh3></p> | ||
+ | <p><hh3> National Science Foundation Grant #1254382</hh3> </p> | ||
+ | <p><hh3> Siemens</hh3></p> | ||
+ | <p><hh3> AGCO</hh3></p> | ||
+ | <p><hh3> Franklin Technologies</hh3></p> | ||
+ | <p><hh3> Gofundme</hh3></p> | ||
+ | <p><hh3> Johns Creek Chick fil A</hh3></p> | ||
+ | <p><hh3> Rosco: Roscolux </hh3></p> | ||
+ | |||
+ | <h2> Wiki Support </h2> | ||
+ | <p><hh3> Joel Joseprabu: Wiki Formatting</hh3> </p> | ||
+ | <p><hh3> Balachandran Srinivasan: Wiki Formatting</hh3> </p> | ||
+ | <h2> Outreach Support </h2> | ||
+ | <p> <hh3> Next Generation Focus/ Grace Chapel Hill Church</hh3></p> | ||
+ | <p> <hh3> Atlanta Science Festival</hh3></p> | ||
+ | <p> <hh3> Atlanta Maker Faire </hh3></p> | ||
+ | <p> <hh3> Sharon Elementary School </hh3></p> | ||
+ | <h2> Sources </h2> | ||
+ | <p>And, S. A. (2009, February 13). Sarita Ahlawat. ClpXP Degrades SsrA-tagged proteins in S.pneumoniae.Retrieved Summer, 2016, from http://jb.asm.org/content/191/8/2894.full | ||
+ | <br><br> | ||
+ | Andersen , J.B. , Sternberg , C. , Poulsen , L.K. , Bjorn , S.P. , Givskov , M. , and Molin , S. ( 1998 ) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria . Appl Environ Microbiol 64 : 2240 – 2246 . | ||
+ | <br><br> | ||
+ | Baker, T. A., & Sauer, R. T. (2011, June 27). ClpXP, an ATP-powered unfolding and protein-degradation machine. Retrieved Summer, 2016, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209554/ | ||
+ | <br><br> | ||
+ | Bar-Nun, S., & Glickman, M. H. (2012). Proteasomal AAA-ATPases: Structure and function. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1823(1), 67–82. doi:10.1016/j.bbamcr.2011.07.009. Retrieved Summer, 2016 from http://www.sciencedirect.com/science/article/pii/S0167488911001984 | ||
+ | <br><br> | ||
+ | Bohn , C. , Binet , E. , and Bouloc , P. ( 2002 ) Screening for stabilization of proteins with a trans-translation signature in Escherichia coli selects for inactivation of the ClpXP protease . Mol Genet Genomics 266 : 827 –831 . | ||
+ | <br><br> | ||
+ | Burton , R.E. , Siddiqui , S.M. , Kim , Y.I. , Baker , T.A. , and Sauer , R.T. ( 2001 ) Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine . EMBO J 20 : 3092 –3100 . | ||
+ | <br><br> | ||
+ | Ciechanover, A. (2005). Cell death and differentiation - abstract of article: Intracellular protein degradation: From a vague idea thru the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting[ast]. Cell Death & Differentiation, 12(9), 1178–1190. doi:10.1038/sj.cdd.4401692 | ||
+ | <br><br> | ||
+ | Cooper, G. M. (2000). Protein degradation. Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/books/NBK9957/ | ||
+ | <br><br> | ||
+ | Dhakar, L. (n.d.). Image Color Picker (Z. A., Ed.). Retrieved October 10, 2016, from http://www.colorcodepicker.com/ | ||
+ | <br><br> | ||
+ | Farrell, C., Grossman, A., & Sauer, R. (2005). Cytoplasmic degradation of ssrA-tagged proteins.Molecular microbiology., 57(6), 1750–61. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16135238 | ||
+ | <br><br> | ||
+ | Flynn , J.M. , Levchenko , I. , Seidel , M. , Wickner , S.H. , Sauer , R.T. , and Baker , T.A. ( 2001 ) Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis . Proc Natl Acad Sci USA 11 : 10584 – 10589. | ||
+ | <br><br> | ||
+ | Georgia Institute of Technology. (2015, September 1). “Bacterial litmus Test” provides inexpensive measurement of Micronutrients. Retrieved from GT News Center, http://www.news.gatech.edu/2015/09/01/bacterial-litmus-test-provides-inexpensive-measurement-micronutrients | ||
+ | <br><br> | ||
+ | Goldberg, A.L., A.S. Menon, S. Goff and D.T. Chin. 1987. The mechanism and regulation of the ATP-dependent protease La from Escherichia coli. Biochem. Soc. Trans. 15: 809-811. Retrieved October 1, 2016 from http://www.fao.org/wairdocs/ilri/x5550e/x5550e0d.htm | ||
+ | <br><br> | ||
+ | Hwang BJ, Woo KM, Goldberg AL, Chung CH. Protease Ti, a new ATP-dependent protease in Escherichia coli,contains protein-activated ATPase and proteolytic functions in distinct subunits. J Biol Chem. 1988;263:8727–8734. | ||
+ | <br><br> | ||
+ | Katayama-Fujimura Y, Gottesman S, Maurizi MR. A multiple-component, ATP-dependent protease from Escherichia coli. J Biol Chem. 1987;262:4477–4485. | ||
+ | <br><br> | ||
+ | Landry, B. P., & Stöckel, J. (2013). Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Applied and Environmental Microbiology,79(8), 2833–2835. doi:10.1128/AEM.03741-12 | ||
+ | <br><br> | ||
+ | Léa, D. (2016). Les meilleures banques en ligne - Banque en ligne de l'Université Cefi. Retrieved October 18, 2016, from http://univ-cefi.fr/les-meilleures-banques-en-ligne.html | ||
+ | <br><br> | ||
+ | Lee C, Schwartz MP, Prakash S, Iwakura M, Matouschek A. ATP-Dependent Proteases Degrade Their Substrates by Processively Unraveling Them from the Degradation Signal. | ||
+ | <br><br> | ||
+ | McNerney, M. P., Watstein, D. M., & Styczynski, M. P. (2015). Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems. Metabolic Engineering, 31, 123–131. doi:10.1016/j.ymben.2015.06.011 | ||
+ | <br><br> | ||
+ | Minikel, E. V. (2013, June 11). Basics of protein degradation. Retrieved Summer, 2016, from http://www.cureffi.org/2013/07/11/basics-of-protein-degradation/ | ||
+ | <br><br> | ||
+ | Mogk A, Schmidt R, Bukau B. The N-end rule pathway for regulated proteolysis: prokaryotic and eukaryotic strategies. Trends Cell Biol. 2007;17:165–172. | ||
+ | <br><br> | ||
+ | Purcell, O., Grierson, C. S., Bernardo, M. di, & Savery, N. J. (2012). Temperature dependence of ssrA-tag mediated protein degradation. Journal of Biological Engineering, 6(1), . doi:10.1186/1754-1611-6-10 | ||
+ | <br><br> | ||
+ | Purple color codes. (n.d.). Retrieved October 10, 2016, from http://www.rapidtables.com/web/color/purple-color.htm | ||
+ | <br><br> | ||
+ | RGB Color Gradient Maker. (n.d.). Retrieved October 10, 2016, from http://www.perbang.dk/rgbgradient/ | ||
+ | <br><br> | ||
+ | Schrader, E. K., Harstad, K. G., & Matouschek, A. (n.d.). Targeting proteins for degradation. , 5(11), . Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228941/ | ||
+ | <br><br> | ||
+ | Snider, J., Thibault, G., & Houry, W. A. (2008). The AAA+ superfamily of functionally diverse proteins. , 9(4), . Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643927/ | ||
+ | <br><br> | ||
+ | Tamura, K., Shimada, T., Ono, E., Tanaka, Y., Nagatani, A., Higashi, S., . . . Hara-Nishimura, I. (2003, September). Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants. The Plant Journal, 35(4), 545-555. doi:10.1046/j.1365-313X.2003.01822.x | ||
+ | <br><br> | ||
+ | Tanaka K. The proteasome: overview of structure and functions. Proc Jpn Acad Ser B Phys Biol Sci.2009;85:12–36. | ||
+ | <br><br> | ||
+ | Tao, L., & Biswas, I. (2015). Degradation of SsrA-tagged proteins in streptococci. , 161(Pt 4),. Retrieved September 9, 2016 from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857447/ | ||
+ | <br><br> | ||
+ | Tu, D., Lee, J., Ozdere, T., Lee, T. J., & You, L. (2007, January ). Engineering Genetic Circuits: Foundations and Applications. Retrieved from http://people.duke.edu/~you/publications/Tu_etal_SyntheticBiology.pdf | ||
+ | <br><br> | ||
+ | Watstein, D. M., McNerney, M. P., & Styczynski, M. P. (2015). Precise metabolic engineering of carotenoid biosynthesis in Escherichia coli towards a low-cost biosensor. Metabolic Engineering,31, 171–180. doi:10.1016/j.ymben.2015.06.007 | ||
+ | <br> | ||
</p> | </p> | ||
+ | <center> <h1 id="MainTitle"><b> Sponsors </b></h1> <img src="https://static.igem.org/mediawiki/2016/2/26/T--LambertGA--purpleline.jpg" style="width:18%; margin:auto;"></center> | ||
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+ | |||
+ | <a href="http://franklin-technologies.com/"><img src="https://static.igem.org/mediawiki/2016/b/b7/T--LambertGA--franklin1.jpg" class="transparent" style="height:120px"></a> | ||
+ | |||
+ | <a href="https://www.gofundme.com/"><img src="https://static.igem.org/mediawiki/2016/f/f6/T--LambertGA--gofundme.jpg" class="transparent" style="height:100px"></a> | ||
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Latest revision as of 20:25, 24 December 2016
Attributions and Acknowledgements
![](https://static.igem.org/mediawiki/2016/2/26/T--LambertGA--purpleline.jpg)
Attributions
Research
Labwork
Wiki
And, S. A. (2009, February 13). Sarita Ahlawat. ClpXP Degrades SsrA-tagged proteins in S.pneumoniae.Retrieved Summer, 2016, from http://jb.asm.org/content/191/8/2894.full
Design
Outreach
Presentation
Acknowledgements
General Support
Parts Support
Funding/Materials Support
Wiki Support
Outreach Support
Sources
Andersen , J.B. , Sternberg , C. , Poulsen , L.K. , Bjorn , S.P. , Givskov , M. , and Molin , S. ( 1998 ) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria . Appl Environ Microbiol 64 : 2240 – 2246 .
Baker, T. A., & Sauer, R. T. (2011, June 27). ClpXP, an ATP-powered unfolding and protein-degradation machine. Retrieved Summer, 2016, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209554/
Bar-Nun, S., & Glickman, M. H. (2012). Proteasomal AAA-ATPases: Structure and function. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1823(1), 67–82. doi:10.1016/j.bbamcr.2011.07.009. Retrieved Summer, 2016 from http://www.sciencedirect.com/science/article/pii/S0167488911001984
Bohn , C. , Binet , E. , and Bouloc , P. ( 2002 ) Screening for stabilization of proteins with a trans-translation signature in Escherichia coli selects for inactivation of the ClpXP protease . Mol Genet Genomics 266 : 827 –831 .
Burton , R.E. , Siddiqui , S.M. , Kim , Y.I. , Baker , T.A. , and Sauer , R.T. ( 2001 ) Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine . EMBO J 20 : 3092 –3100 .
Ciechanover, A. (2005). Cell death and differentiation - abstract of article: Intracellular protein degradation: From a vague idea thru the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting[ast]. Cell Death & Differentiation, 12(9), 1178–1190. doi:10.1038/sj.cdd.4401692
Cooper, G. M. (2000). Protein degradation. Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/books/NBK9957/
Dhakar, L. (n.d.). Image Color Picker (Z. A., Ed.). Retrieved October 10, 2016, from http://www.colorcodepicker.com/
Farrell, C., Grossman, A., & Sauer, R. (2005). Cytoplasmic degradation of ssrA-tagged proteins.Molecular microbiology., 57(6), 1750–61. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16135238
Flynn , J.M. , Levchenko , I. , Seidel , M. , Wickner , S.H. , Sauer , R.T. , and Baker , T.A. ( 2001 ) Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis . Proc Natl Acad Sci USA 11 : 10584 – 10589.
Georgia Institute of Technology. (2015, September 1). “Bacterial litmus Test” provides inexpensive measurement of Micronutrients. Retrieved from GT News Center, http://www.news.gatech.edu/2015/09/01/bacterial-litmus-test-provides-inexpensive-measurement-micronutrients
Goldberg, A.L., A.S. Menon, S. Goff and D.T. Chin. 1987. The mechanism and regulation of the ATP-dependent protease La from Escherichia coli. Biochem. Soc. Trans. 15: 809-811. Retrieved October 1, 2016 from http://www.fao.org/wairdocs/ilri/x5550e/x5550e0d.htm
Hwang BJ, Woo KM, Goldberg AL, Chung CH. Protease Ti, a new ATP-dependent protease in Escherichia coli,contains protein-activated ATPase and proteolytic functions in distinct subunits. J Biol Chem. 1988;263:8727–8734.
Katayama-Fujimura Y, Gottesman S, Maurizi MR. A multiple-component, ATP-dependent protease from Escherichia coli. J Biol Chem. 1987;262:4477–4485.
Landry, B. P., & Stöckel, J. (2013). Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Applied and Environmental Microbiology,79(8), 2833–2835. doi:10.1128/AEM.03741-12
Léa, D. (2016). Les meilleures banques en ligne - Banque en ligne de l'Université Cefi. Retrieved October 18, 2016, from http://univ-cefi.fr/les-meilleures-banques-en-ligne.html
Lee C, Schwartz MP, Prakash S, Iwakura M, Matouschek A. ATP-Dependent Proteases Degrade Their Substrates by Processively Unraveling Them from the Degradation Signal.
McNerney, M. P., Watstein, D. M., & Styczynski, M. P. (2015). Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems. Metabolic Engineering, 31, 123–131. doi:10.1016/j.ymben.2015.06.011
Minikel, E. V. (2013, June 11). Basics of protein degradation. Retrieved Summer, 2016, from http://www.cureffi.org/2013/07/11/basics-of-protein-degradation/
Mogk A, Schmidt R, Bukau B. The N-end rule pathway for regulated proteolysis: prokaryotic and eukaryotic strategies. Trends Cell Biol. 2007;17:165–172.
Purcell, O., Grierson, C. S., Bernardo, M. di, & Savery, N. J. (2012). Temperature dependence of ssrA-tag mediated protein degradation. Journal of Biological Engineering, 6(1), . doi:10.1186/1754-1611-6-10
Purple color codes. (n.d.). Retrieved October 10, 2016, from http://www.rapidtables.com/web/color/purple-color.htm
RGB Color Gradient Maker. (n.d.). Retrieved October 10, 2016, from http://www.perbang.dk/rgbgradient/
Schrader, E. K., Harstad, K. G., & Matouschek, A. (n.d.). Targeting proteins for degradation. , 5(11), . Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228941/
Snider, J., Thibault, G., & Houry, W. A. (2008). The AAA+ superfamily of functionally diverse proteins. , 9(4), . Retrieved Summer, 2016 from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643927/
Tamura, K., Shimada, T., Ono, E., Tanaka, Y., Nagatani, A., Higashi, S., . . . Hara-Nishimura, I. (2003, September). Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants. The Plant Journal, 35(4), 545-555. doi:10.1046/j.1365-313X.2003.01822.x
Tanaka K. The proteasome: overview of structure and functions. Proc Jpn Acad Ser B Phys Biol Sci.2009;85:12–36.
Tao, L., & Biswas, I. (2015). Degradation of SsrA-tagged proteins in streptococci. , 161(Pt 4),. Retrieved September 9, 2016 from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857447/
Tu, D., Lee, J., Ozdere, T., Lee, T. J., & You, L. (2007, January ). Engineering Genetic Circuits: Foundations and Applications. Retrieved from http://people.duke.edu/~you/publications/Tu_etal_SyntheticBiology.pdf
Watstein, D. M., McNerney, M. P., & Styczynski, M. P. (2015). Precise metabolic engineering of carotenoid biosynthesis in Escherichia coli towards a low-cost biosensor. Metabolic Engineering,31, 171–180. doi:10.1016/j.ymben.2015.06.007
Sponsors