Difference between revisions of "Team:Stanford-Brown/SB16 Notebooks Nylon"

Line 61: Line 61:
 
                  <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Float<span class="caret"></span></a>
 
                  <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Float<span class="caret"></span></a>
 
                  <ul class="dropdown-menu">
 
                  <ul class="dropdown-menu">
−
                    <li><a href="https://2016.igem.org/Team:Stanford-Brown/SB16_Float_Overview">Overview</a></li>
 
 
<li><a href="https://2016.igem.org/Team:Stanford-Brown/SB16_Float_Gas">Gas production</a></li>
 
<li><a href="https://2016.igem.org/Team:Stanford-Brown/SB16_Float_Gas">Gas production</a></li>
 
                  </ul>
 
                  </ul>

Revision as of 03:28, 17 October 2016


Stanford-Brown 2016

Nylon/Styrene-Butadiene Rubber/Other Materials · Benchling

Nylon/Styrene-Butadiene Rubber/Other Materials

Made with Benchling
Project: iGEM 2016
Authors: Michael Becich, Elias Robinson
Dates: 2016-06-29 to 2016-07-11
Wednesday, 6/29
-Cryostocked E. coli strains (eAKP-71 and eAKP-744) expressing enzymes from biosynthesis pathway for 6-Aminocaproic Acid from Stefan Turk (DSM Food Specialties B.V., Netherlands) arrived to the lab
-Made four liquid cultures, half incubated at 37C at 220 rpm to stimulate rapid division and plasmid copy, and half at room temp and ~60 rpm to optimize effectiveness of oxygen sensitive chain elongation enzymes (AksD/E complex)
Thursday, 6/30
-Miniprepped plasmids from eAKP-71 and eAKP-744 (Elias)
-Ordered primers for PCR amplification of promoters, RBSs, and operons.
Monday, 7/11
●
Anioninc Polymerization vs. Emulison Polymeriation of Butadiene
●
Heat Ethanol to high temperature