Team:Stanford-Brown/SB16 Notebooks Interlab

iGEM (InterLab, Cell Competency Kit, BioBrick Characterizations, etc.)

Made with Benchling

Project: iGEM 2016

Authors: Taylor Sihavong, Charles Gleason, Michael Becich

Dates: 2016-06-16 to 2016-09-06

Thursday, 6/16/16

-Cell competency test done on 3 different DNA concentrations (0.5pg/ul, 10pg/ul, 50pg/ul) with 2 different cell types (T7, 5-alpha)

-Protocol given for the competency test wasn't compatible with T7, but we got colonies on all of our 5-alpha plates (Charlie, Taylor)

Monday, 6/20

-Began InterLab study. Started with the high efficiency transformation protocol for NEB 5-alpha Competent E. Coli. Transformed 5 DNA samples: positive control, negative control, and 3 test devices. (Taylor, Cynthia, Amy)

-Test device 3 was diluted with 100ul DI water with 5ul of DNA taken out

-Plated with 1:4 dilutions

Tuesday, 6/21

-InterLab study results: test device 1 plate had no colonies --> store positive control, negative control, test device 2, test device 3 at -4˚C (Charlie)

IMG_2671.JPG

-Redo transformation of test device 1 and test device 3

-Test device 1 was diluted with 100ul DI water with 5ul of DNA taken out

-2 plates each, a 4X dilution and a 40X dilution

Tuesday, 6/28

-Team Brainstorming Session: Where are we at and where are we heading?

IMG_2634.JPG

Thursday, 6/30

-Performed calibration plate readings (2 plates) for interlab study (Taylor S)

-Prepared overnight cultures for interlab study (2 colonies per plate, 5 plates) (Mike)

Friday, 7/1

-Made Terrific Broth (TB, 0.17M KH₂PO₄, 0.72M K₂HPO₄) buffer for interlab study, Prepared Interlab Samples for Weekend Storage

Tuesday, 7/5

- Worked on part 2 of Interlab Measurement Study for 6 hours, measured fluorescence after 6 hours with SpectraMax Plus 384

Wednesday, 7/20

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37th Anniversary of Putting First Human on the Moon!

Thursday, 7/21

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NASA Star Trek Premiere Special Showing!

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Jim Head Presentation and Q&A (Mars Climate Change, Psychology of Family of Planets)

Friday, 7/22

Saturday, 7/23

-Hackathon to learn methods for improving website

Tuesday, 9/6

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Freeze dried 10 uL of first 8 BioBricks!

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1st Submission: rAIP, AE Blue, 1 np Et?, Gb2, c1, c2, c3, cw