Difference between revisions of "Team:LambertGA/Results"

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<center><h3 style="color: #D49AE6;">Inoculations of GFP Constructs (58 tubes)</h3></center>
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After making our constructs, we inoculated cultures from previous transformations that have successfully expressed the fluorescent protein.
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1.P-Lambda-R--LacI--GFP (no deg tag)
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<OL TYPE="a">
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<LI>3 tubes in each cell type (DH10, Keio Wild, Keio ClpP) induced with 0 uM IPTG (plain LB)
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<LI>3 tubes of each cell type (DH10, Keio Wild, Keio ClpP) induced with 100 uM IPTG (plain LB)
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<LI>RESULTS:
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</OL>
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2. P-Lambda-R--LacI--GFP (DAS)
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<OL TYPE="a">
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<LI>3 tubes in each cell type (DH10, Keio Wild, Keio ClpP) induced with 0 uM IPTG (plain LB)
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<LI>3 tubes of each cell type (DH10, Keio Wild, Keio ClpP) induced with 100 uM IPTG (plain LB)
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<LI>RESULTS:
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</OL>
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3. P-Lambda-R--LacI--GFP (LAA)
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<OL TYPE="a">
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<LI>3 tubes in each cell type (DH10, Keio Wild, Keio ClpP) induced with 0 uM IPTG (plain LB)
 +
<LI>3 tubes of each cell type (DH10, Keio Wild, Keio ClpP) induced with 100 uM IPTG (plain LB)
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<LI>RESULTS:
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</OL>
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4. Plain LB, DH10 cells in plain LB, Keio Wild cells in plain LB, and Keio ClpP cells in plain LB
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5. RESULTS:
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<OL TYPE="a">
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<LI> 10/17: The GFP constructs were brought to the plate reader at Georgia Tech.  Although cells were grown in the liquid culture, they did not fluoresce.
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</OL>
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6. Pictures:
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<br>
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<img src"https://static.igem.org/mediawiki/2016/e/e8/T--LambertGA--platereaderresults.jpg">
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<br>
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</p>
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<center><h3 style="color: #D49AE6;">Relative Color Quantification</h3></center>
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<p style="width: 100%; margin: auto; font-size: 16px;">Relative strength of the degradation of tsPurple cells (control, TS Purple DAS, and TS Purple LAA) can be measured by using a smartphone camera lens or any simple photography device (along with the color-controls for consistency).
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<center> <h1 id="MainTitle"><b> Results </b></h1>
 
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<img src="https://static.igem.org/mediawiki/2016/2/26/T--LambertGA--purpleline.jpg" style="width:18%; margin:auto;"> </center>
 
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Revision as of 04:14, 19 October 2016

Inoculations of GFP Constructs (58 tubes)

After making our constructs, we inoculated cultures from previous transformations that have successfully expressed the fluorescent protein.
1.P-Lambda-R--LacI--GFP (no deg tag)

  1. 3 tubes in each cell type (DH10, Keio Wild, Keio ClpP) induced with 0 uM IPTG (plain LB)
  2. 3 tubes of each cell type (DH10, Keio Wild, Keio ClpP) induced with 100 uM IPTG (plain LB)
  3. RESULTS:
2. P-Lambda-R--LacI--GFP (DAS)
  1. 3 tubes in each cell type (DH10, Keio Wild, Keio ClpP) induced with 0 uM IPTG (plain LB)
  2. 3 tubes of each cell type (DH10, Keio Wild, Keio ClpP) induced with 100 uM IPTG (plain LB)
  3. RESULTS:
3. P-Lambda-R--LacI--GFP (LAA)
  1. 3 tubes in each cell type (DH10, Keio Wild, Keio ClpP) induced with 0 uM IPTG (plain LB)
  2. 3 tubes of each cell type (DH10, Keio Wild, Keio ClpP) induced with 100 uM IPTG (plain LB)
  3. RESULTS:
4. Plain LB, DH10 cells in plain LB, Keio Wild cells in plain LB, and Keio ClpP cells in plain LB 5. RESULTS:
  1. 10/17: The GFP constructs were brought to the plate reader at Georgia Tech. Although cells were grown in the liquid culture, they did not fluoresce.
6. Pictures:


Relative Color Quantification

Relative strength of the degradation of tsPurple cells (control, TS Purple DAS, and TS Purple LAA) can be measured by using a smartphone camera lens or any simple photography device (along with the color-controls for consistency).