Difference between revisions of "Team:LambertGA/Proof"
| Line 304: | Line 304: | ||
<a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a> | <a href="https://2016.igem.org/Team:LambertGA/Team" class="dropbtn">Team</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
| + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Team">Team</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Collaborations">Collaborations</a> | ||
</div> | </div> | ||
| Line 321: | Line 322: | ||
<a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a> | <a href="https://2016.igem.org/Team:LambertGA/Parts" class="dropbtn">Parts</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
| + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Parts">Parts</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Basic_Part">Basic Parts</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Composite_Part">Composite Parts</a> | ||
| Line 330: | Line 332: | ||
<a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a> | <a href="https://2016.igem.org/Team:LambertGA/Human_Practices" class="dropbtn">Human Practices</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
| + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/Human_Practices">Human Practices</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Silver">Silver</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2016.igem.org/Team:LambertGA/HP/Gold">Gold</a> | ||
Revision as of 21:07, 19 October 2016
Proof Of Concept
Prior Work
These results were obtained using overnight cultures grown at 37C in a shaking incubator at 170rpm. The values were normalized using control tubes of LB and Keio ClpP cells grown under the same conditions. The strains that were grown were the full “Switch” constructs with GFP DAS and GFP LAA degradation tags. Unfortunately, the control cells of GFP without a tag did not grow. The initial results seem to indicate that under induction of IPTG the level of GFP protein does indeed decrease. Further experimentation will be conducted to include a control construct without a degradation tag as well as a comparison to Keio Wild type strain.
