Difference between revisions of "Team:NCKU Tainan/Model"

m (Add model link)
Line 1: Line 1:
 
<html><head>
 
<html><head>
     <meta charset="utf-8"><link rel="shortcut icon" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png" type="image/x-icon"><link rel="icon" type="image/png" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png"><link rel="icon" type="image/x-icon" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png"><meta name="viewport" content="width=device-width, initial-scale=1.0"><meta property="og:title" content="Project Modeling - iGEM NCKU"><meta property="og:site_name" content="Project Modeling - iGEM NCKU"><meta property="og:description" content=""><title>Project Modeling - iGEM NCKU</title><meta http-equiv="Content-Type" content="text/html" charset="utf-8"><meta property="og:image" content=""><meta property="og:image:type" content="image/png"><link rel="stylesheet" href="/Team:NCKU_Tainan/css/frame/T--NCKU_Tainan--bootstrap_min_css?ctype=text/css&amp;action=raw"><link href="/Team:NCKU_Tainan/font/T--NCKU_Tainan--NotoSans_css?ctype=text/css&amp;action=raw" rel="stylesheet" type="text/css"><link rel="stylesheet" href="/Team:NCKU_Tainan/font/T--NCKU_Tainan--font-awesome_min_css?ctype=text/css&amp;action=raw"> <link rel="stylesheet" href="/Team:NCKU_Tainan/css/T--NCKU_Tainan--Layout_css?ctype=text/css&amp;action=raw">
+
     <meta charset="utf-8"><link rel="shortcut icon" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png" type="image/x-icon"><link rel="icon" type="image/png" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png"><link rel="icon" type="image/x-icon" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png"><meta name="viewport" content="width=device-width, initial-scale=1.0"><meta property="og:title" content="Judging Mdeal - iGEM NCKU"><meta property="og:site_name" content="Judging Mdeal - iGEM NCKU"><meta property="og:description" content=""><title>Judging Mdeal - iGEM NCKU</title><meta http-equiv="Content-Type" content="text/html" charset="utf-8"><meta property="og:image" content=""><meta property="og:image:type" content="image/png"><link rel="stylesheet" href="/Team:NCKU_Tainan/css/frame/T--NCKU_Tainan--bootstrap_min_css?ctype=text/css&amp;action=raw"><link href="/Team:NCKU_Tainan/font/T--NCKU_Tainan--NotoSans_css?ctype=text/css&amp;action=raw" rel="stylesheet" type="text/css"><link rel="stylesheet" href="/Team:NCKU_Tainan/font/T--NCKU_Tainan--font-awesome_min_css?ctype=text/css&amp;action=raw"> <link rel="stylesheet" href="/Team:NCKU_Tainan/css/T--NCKU_Tainan--Medal_css?ctype=text/css&amp;action=raw">
 
   </head>
 
   </head>
 
   <body>
 
   <body>
     <style>@font-face {  font-family: 'NotoSansCJKtc-Regular';  src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div class="container-fluid" style="width:72%">  <div class="navbar-header">      <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false">        <span class="sr-only">Toggle navigation</span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>      </button>      <a class="navbar-brand" href="/Team:NCKU_Tainan">        <h1>NCKU</h1><h4>Tainan</h4>      </a>  </div>  <div id="navbar" class="navbar-collapse collapse">    <ul class="nav navbar-nav">      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Project">Overview</a></li>          <li><a href="">Description</a></li>          <li><a href="/Team:NCKU_Tainan/Results">Results</a></li>          <li><a href="/Team:NCKU_Tainan/Model">Modeling</a></li>          <li><a href="/Team:NCKU_Tainan/Parts">Parts</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu2" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Device</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="">Hardware</a></li>          <li><a href="">Software</a></li>          <li><a href="">Demonstrate</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu3" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Judging</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu3">          <li><a href="/Team:NCKU_Tainan/Medal">Medal</a></li>          <li><a href="/Team:NCKU_Tainan/Safety">Safety</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu4" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Team">Team</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu4">          <li><a href="/Team:NCKU_Tainan/Team">Team</a></li>          <li><a href="/Team:NCKU_Tainan/Attributions">Attributions</a></li>          <li><a href="/Team:NCKU_Tainan/Acknowledgement">Acknowledgement</a></li>          <li><a href="">Collaborations</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu5" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Human_Practices">Human Practices</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu5">          <li><a href="/Team:NCKU_Tainan/Human_Practices">Overview</a></li>          <li><a href="">Integrated Practices</a></li>          <li><a href="">Engagement</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu6" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Notebook</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu6">          <li><a href="">Construction</a></li>          <li><a href="">Functional Test</a></li>          <li><a href="#">Device Design</a></li>          <li><a href="#">Protocols</a></li>        </ul>      </li>    </ul>    <ul class="nav navbar-nav navbar-right">    </ul>  </div><!--/.nav-collapse --></div><!--/.container-fluid --></nav><div id="container-big"><div id="iGEMbar"></div><div id="line-left"></div><div id="line-left2"></div><div id="line-right"></div><div id="photo-left"></div></div><!--/.container-big --> <style>
+
     <style>@font-face {  font-family: 'NotoSansCJKtc-Regular';  src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div class="container-fluid" style="width:72%">  <div class="navbar-header">      <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false">        <span class="sr-only">Toggle navigation</span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>      </button>      <a class="navbar-brand" href="/Team:NCKU_Tainan">        <h1>NCKU</h1><h4>Tainan</h4>      </a>  </div>  <div id="navbar" class="navbar-collapse collapse">    <ul class="nav navbar-nav">      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Project">Overview</a></li>          <li><a href="/Team:NCKU_Tainan/Description">Description</a></li>          <li><a href="/Team:NCKU_Tainan/Results">Results</a></li>          <li><a href="/Team:NCKU_Tainan/Model">Modeling</a></li>          <li><a href="/Team:NCKU_Tainan/Parts">Parts</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu2" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Device</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Hardware">Hardware</a></li>          <li><a href="/Team:NCKU_Tainan/Software">Software</a></li>          <li><a href="/Team:NCKU_Tainan/Demonstrate">Demonstrate</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu3" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Judging</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu3">          <li><a href="/Team:NCKU_Tainan/Medal">Medal</a></li>          <li><a href="/Team:NCKU_Tainan/Safety">Safety</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu4" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Team">Team</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu4">          <li><a href="/Team:NCKU_Tainan/Team">Team</a></li>          <li><a href="/Team:NCKU_Tainan/Attributions">Attributions</a></li>          <li><a href="/Team:NCKU_Tainan/Acknowledgement">Acknowledgement</a></li>          <li><a href="/Team:NCKU_Tainan/Collaborations">Collaborations</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu5" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Human_Practices">Human Practices</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu5">          <li><a href="/Team:NCKU_Tainan/Human_Practices">Overview</a></li>          <li><a href="/Team:NCKU_Tainan/Integrated_Practices">Integrated Practices</a></li>          <li><a href="/Team:NCKU_Tainan/Engagement">Engagement</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu6" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Notebook</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu6">          <li><a href="/Team:NCKU_Tainan/Notebook_Construction">Construction</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Functional_Test">Functional Test</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Device_Design">Device Design</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Protocols">Protocols</a></li>        </ul>      </li>    </ul>    <ul class="nav navbar-nav navbar-right">    </ul>  </div><!--/.nav-collapse --></div><!--/.container-fluid --></nav><div id="container-big"><div id="iGEMbar"></div><div id="line-left"></div><div id="line-left2"></div><div id="line-right"></div><div id="photo-left"></div></div><!--/.container-big --> <style>
 
#photo-left { background-image: url("/wiki/images/c/c1/T--NCKU_Tainan--sample2.jpg"); }
 
#photo-left { background-image: url("/wiki/images/c/c1/T--NCKU_Tainan--sample2.jpg"); }
 
</style>
 
</style>
 
<div class="container-fluid" style="margin-top:100px">
 
<div class="container-fluid" style="margin-top:100px">
<div class="head">PROJECT / Model</div>
+
<div class="head">JUDGING / Medal</div>
 
<div class="content row">
 
<div class="content row">
<div class="col-md-9">
+
          <div class="col-md-9">
<div class="head2">Model</div>
+
<div class="head2">Medal</div>
<div class="title-line" id="fitting">Fitting Theory</div>
+
          <h4 class="title-line" id="bronze">Bronze</h4>
            <p>U.coli uses promoter Pl, which can be inhibited by CRP and indirectly induced by glucose, to produce fluorescent protein. Because the process that glucose induces the promoter is not a direct process, there are a lot of compound involve in it and form a network.</p>
+
            <h5>1.   Register and Attend </h5>
            <p> This network is composed of two main systems: glucose digest system and CRP activation system, which are competitive to each other. </p>
+
            <p>As an "undergraduate" and "first" team from southern Taiwan, NCKU Tainan team registered for iGEM 2016 competition.</p>
<div class="img" style="width:32%;display:inline-block;margin-left:8px">
+
            <p></p>
            <img src="/wiki/images/b/b7/T--NCKU_Tainan--project-modeling-fitting-image1.jpg">
+
            <h5>2.   Deliverables</h5>
            <div>The network of induction of glucose induced promoter Pl.</div>
+
            <p>We completed all the deliverables and are going to the iGEM 2016 Giant Jamboree.
</div>
+
            Specific information can be found in <a href="/Team:NCKU_Tainan/Attributions">Attribution</a> / <a href="/Team:NCKU_Tainan/Parts">Parts and Submission</a> / <a href="judgingSafety">Safety</a> / <a href="judgingMedal">Judging</a>
<div class="img" style="width:32%;display:inline-block">
+
            </p>
            <img src="/wiki/images/f/f7/T--NCKU_Tainan--project-modeling-fitting-image2.jpg">
+
            <h5>3.   Attribution</h5>
            <div>Two different pathway in the network.</div>
+
            <p>The individual attributions of each team member are well documented in our <a href="/Team:NCKU_Tainan/Attributions">Attribution Page</a>, For contributions and aid from our mentors and supporters, please see <a href="/Team:NCKU_Tainan/Acknowledgement">Acknowledgement</a>.
            </div>
+
            </p>
<div class="img" style="width:32%;display:inline-block">
+
            <h5>4.   Part / Contribution</h5>
            <img src="/wiki/images/0/08/T--NCKU_Tainan--project-modeling-fitting-image3.jpg">
+
            <p>Our project aims to prevent diabetes by detecting urine glucose in a non-invasive way. The <a href="/Team:NCKU_Tainan/Parts">parts</a> that we built are well documented and already submitted.
            <div>The overall impact when glucose is added.</div>
+
            </p>
            </div>
+
            <p> When glucose is added, some related compound will decrease during the reaction chain, and finally the concentration of activated CRP will also decrease, which means the CRP inhibited promoter Pl can express more following gene with the addition of glucose.</p>
+
            <p>Obviously, the concentration of adding glucose and the activated CRP have negative correlation. But if we want to assay the concentration of inputted glucose numerically, we have to derive a formula base on these chemical pathways.</p>
+
<p>(<a href="/Team:NCKU_Tainan/Model_Fitting_Theory">Read more...</a>)</p>
+
  
<div class="title-line" id="statistics">Statistics Analysis</div>
+
 
                  <p>In medicine, the blood glucose exceeding 5mM/L implies having diabetes, and it exceeding 120mM/L implies being a severe patient. Finding the person whose glucose concentration is over 5mM/L or 120mM/L is our target.</p>
+
          <h4 class="title-line" id="silver">Silver</h4>
<div class="img" style="width:32%;display:inline-block;margin-left:8px">
+
            <h5>1.    Validated Part / Validated Contribution</h5>
            <img src="/wiki/images/c/c0/T--NCKU_Tainan--project-modeling-statistic-image1.jpg">
+
            <p>We experimentally validated our BioBrick device for Urine Glucose Detection (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1920001">BBa_K1920001</a>)and Safety((<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1920006">BBa_K1920006</a>).
              <div>0.1 vs 5 mM/L</div>
+
            For our Detection (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1920001">BBa_K1920001</a>) BioBrick part, we characterized the relationship between glucose concentration and induced RFP fluorescent intensity. And for our Safety((<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1920006">BBa_K1920006</a>) BioBrick part we measured the growth curve and performed a CFU assay.
</div>
+
          </p>
<div class="img" style="width:32%;display:inline-block">
+
          <h5>2.    Collaboration</h5>
            <img src="/wiki/images/c/c0/T--NCKU_Tainan--project-modeling-statistic-image1.jpg">
+
          <p>we interacted with over ??? other iGEM teams in a variety of activities! And our key contributions to other iGEM teams project are as follows:
              <div>0.1 vs 120 mM/L</div>
+
            (1)    Collaborating with <a href="https://2016.igem.org/Team:TCU_Taiwan" target="_blank">TCU_Taiwan</a>, we shared the protocol of Glucose-free modified M9 medium for cell culturing and functional experiments
            </div>
+
            (2)    Collaborating with <a href="https://2016.igem.org/Team:TCU_Taiwan" target="_blank">TCU_Taiwan</a>, we helped the calculation of Enzyme Kinetics and Promoter Efficiency in the <a href="/Team:NCKU_Tainan/Model">modeling</a> phase.
<div class="img" style="width:32%;display:inline-block">
+
            All the details can be found in  <a href="/Team:NCKU_Tainan/Collaborations">Collaboration</a></p>
            <img src="/wiki/images/a/a3/T--NCKU_Tainan--project-modeling-statistic-image3.jpg">
+
            <h5>3.   Human Practices</h5>
              <div>0.1 model plot</div>
+
            <p>In addition to perfecting our project and preventing diabetes, we held a variety of activities to connect our device, U-KNOW, and iGEM to people
            </div>
+
            (1)    We launched videos via social media channels to provide health education and to bust the common myths about diabetes. Also correct information from doctors and specialists were spread through those videos.
                  <p>First, we prove that there has difference between 0.1mM/L and 5mM/L (120mM/L) in the paired-difference T test part. And, we use the regression and prediction intervals to distinguish exceeding 120mM/L and 5mM/L from exceeding 0.1mM/L. From the result, 5mM/L can be distinguished from 0.1mM/L after 101 minutes, and 120mM/L can be distinguished from 0.1mM/L after 88 minutes.</p>
+
            (2)    We held synthetic biology workshops in our university, NCKU, to promote synthetic biology and interdisciplinary cooperation.
+
 
<p>(<a href="/Team:NCKU_Tainan/Model_Statistics_Analysis">Read more...</a>)</p>
+
            for further information, please go to <a href="/Team:NCKU_Tainan/Human_Practices">Human Practices</a></p>
 +
 
 +
 
 +
 
 +
            <h4 class="title-line" id="gold">Gold</h4>
 +
            <h5>1.    Integrated Human Practices</h5>
 +
            <p>This summer we proudly hosted <a href="http://igem.ncku.edu.tw/2016conf/">iGEM 2016 Asia-Pacific Conference</a>, gathering over 20 teams, 180 iGEMers, from three different regions. During the conference, all teams and iGEMers had the opportunity to present their own project as well as to realize to others' project. In addition, we invited experts and specialists from the fields of industry and media to share their perspectives from the different aspects. It is also good for us to seek the opportunity for university-industry collaboration. Within these campaigns, a great opportunity was offered for iGEMers to interact with each other, and we received a lot of positive good responses! So our team has not only performed the lab work but also gone beyond the lab.
 +
            </p>
 +
            <h5>2.   Proof of Concept</h5>
 +
            <p>To prove that U-KNOW can prevent diabetes by detecting, we performed several <a href="/Team:NCKU_Tainan/Model_Fitting_Theory">Urine Functional Tests</a>.  Our Detection Circuit(<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1920001">BBa_K1920001</a>) do differentiate Diabetic urine samples with Control, and the sensitivity can reach 95%, which was already further improved to 100%.</p>
 +
            <p>For Safety, we tested our Safety Circuit(<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1920006">BBa_K1920006</a>) and proved that our design can protect U-KNOW users as well as the environment from bacteria contamination.
 +
          </p>
 +
          <p>To know more, please look at <a href="/Team:NCKU_Tainan/Results">Result/Proof</a></p>
 +
 
 +
            <h5>3.    Demonstrate Your Work</h5>
 +
            <p>To bring U-KNOW to the real world condition, we built different versions of <a href="/Team:NCKU_Tainan/Hardware">device prototypes</a>.
 +
            We believe welcome you to become a U-KNOW user, "Prevention is better than cure", so easy and early detection by using U-KNOW is helpful for pre-diabetes management.
 +
            U-KNOW Makes You Know !</p>
 
</div>
 
</div>
 
<div class="col-md-3">
 
<div class="col-md-3">
 
<ul id="sidemenu">
 
<ul id="sidemenu">
<li><a href="#" onclick="return toEvent('fitting');">Fitting Theory</a></li>
+
<li><a href="#" onclick="return toEvent('bronze');"><span class="medal-block" style="background:#cd7f32;"></span>Bronze</a></li>
<li><a href="#" onclick="return toEvent('statistics');">Statistics Analysis</a></li>
+
<li><a href="#" onclick="return toEvent('silver');"><span class="medal-block" style="background:#c0c0c0;"></span>Silver</a></li>
 +
<li><a href="#" onclick="return toEvent('gold');"><span class="medal-block" style="background:#ffd700"></span>Gold</a></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
 
</div>
 
</div>
 
</div> <!-- /.container-fluid -->
 
</div> <!-- /.container-fluid -->
<script>document.getElementById("line-left2").style.display="none";</script>
+
<script src="/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--jquery-1_12_0_min_js?ctype=text/javascript&amp;action=raw"></script><script src="/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--bootstrap_min_js?ctype=text/javascript&amp;action=raw"></script><script src="/Team:NCKU_Tainan/js/T--NCKU_Tainan--MathjaxConfigIgem_js?ctype=text/javascript&amp;action=raw"></script><script src="/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><script src="/Team:NCKU_Tainan/js/T--NCKU_Tainan--common_js?ctype=text/javascript&amp;action=raw"></script><script>(function() {    /* change icon */    var link = document.createElement('link');    link.type = 'image/x-icon';    link.rel = 'shortcut icon';    link.href = '/wiki/images/8/80/T--NCKU_Tainan--favicon.png';    document.getElementsByTagName('head')[0].appendChild(link);}());</script>
<script src="/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--jquery-1_12_0_min_js?ctype=text/javascript&amp;action=raw"></script><script src="/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--bootstrap_min_js?ctype=text/javascript&amp;action=raw"></script><script type="text/x-mathjax-config">MathJax.Hub.Config({  tex2jax: {inlineMath: [['$','$'], ['\\(','\\)']]},  "HTML-CSS": {scale: 100},  CommonHTML: {scale: 100},  SVG: {scale:100}});</script><script src="https://2016.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><script src="/Team:NCKU_Tainan/js/T--NCKU_Tainan--common_js?ctype=text/javascript&amp;action=raw"></script><script>(function() {    /* change icon */    var link = document.createElement('link');    link.type = 'image/x-icon';    link.rel = 'shortcut icon';    link.href = '/wiki/images/8/80/T--NCKU_Tainan--favicon.png';    document.getElementsByTagName('head')[0].appendChild(link);}());</script>
+
  
 
</body></html>
 
</body></html>

Revision as of 19:23, 9 October 2016

Judging Mdeal - iGEM NCKU

JUDGING / Medal
Medal

Bronze

1. Register and Attend

As an "undergraduate" and "first" team from southern Taiwan, NCKU Tainan team registered for iGEM 2016 competition.

2. Deliverables

We completed all the deliverables and are going to the iGEM 2016 Giant Jamboree. Specific information can be found in Attribution / Parts and Submission / Safety / Judging

3. Attribution

The individual attributions of each team member are well documented in our Attribution Page, For contributions and aid from our mentors and supporters, please see Acknowledgement.

4. Part / Contribution

Our project aims to prevent diabetes by detecting urine glucose in a non-invasive way. The parts that we built are well documented and already submitted.

Silver

1. Validated Part / Validated Contribution

We experimentally validated our BioBrick device for Urine Glucose Detection (BBa_K1920001)and Safety((BBa_K1920006). For our Detection (BBa_K1920001) BioBrick part, we characterized the relationship between glucose concentration and induced RFP fluorescent intensity. And for our Safety((BBa_K1920006) BioBrick part we measured the growth curve and performed a CFU assay.

2. Collaboration

we interacted with over ??? other iGEM teams in a variety of activities! And our key contributions to other iGEM teams project are as follows: (1) Collaborating with TCU_Taiwan, we shared the protocol of Glucose-free modified M9 medium for cell culturing and functional experiments (2) Collaborating with TCU_Taiwan, we helped the calculation of Enzyme Kinetics and Promoter Efficiency in the modeling phase. All the details can be found in Collaboration

3. Human Practices

In addition to perfecting our project and preventing diabetes, we held a variety of activities to connect our device, U-KNOW, and iGEM to people (1) We launched videos via social media channels to provide health education and to bust the common myths about diabetes. Also correct information from doctors and specialists were spread through those videos. (2) We held synthetic biology workshops in our university, NCKU, to promote synthetic biology and interdisciplinary cooperation. for further information, please go to Human Practices

Gold

1. Integrated Human Practices

This summer we proudly hosted iGEM 2016 Asia-Pacific Conference, gathering over 20 teams, 180 iGEMers, from three different regions. During the conference, all teams and iGEMers had the opportunity to present their own project as well as to realize to others' project. In addition, we invited experts and specialists from the fields of industry and media to share their perspectives from the different aspects. It is also good for us to seek the opportunity for university-industry collaboration. Within these campaigns, a great opportunity was offered for iGEMers to interact with each other, and we received a lot of positive good responses! So our team has not only performed the lab work but also gone beyond the lab.

2. Proof of Concept

To prove that U-KNOW can prevent diabetes by detecting, we performed several Urine Functional Tests. Our Detection Circuit(BBa_K1920001) do differentiate Diabetic urine samples with Control, and the sensitivity can reach 95%, which was already further improved to 100%.

For Safety, we tested our Safety Circuit(BBa_K1920006) and proved that our design can protect U-KNOW users as well as the environment from bacteria contamination.

To know more, please look at Result/Proof

3. Demonstrate Your Work

To bring U-KNOW to the real world condition, we built different versions of device prototypes. We believe welcome you to become a U-KNOW user, "Prevention is better than cure", so easy and early detection by using U-KNOW is helpful for pre-diabetes management. U-KNOW Makes You Know !