Difference between revisions of "Team:LambertGA/Measurement"
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<center> <h1 id="MainTitle"><b> Measurement </b></h1><img src="https://static.igem.org/mediawiki/2016/2/26/T--LambertGA--purpleline.jpg" style="width:18%; margin:auto;"> </center> <br> | <center> <h1 id="MainTitle"><b> Measurement </b></h1><img src="https://static.igem.org/mediawiki/2016/2/26/T--LambertGA--purpleline.jpg" style="width:18%; margin:auto;"> </center> <br> | ||
| + | <center><h3 style="color: #D49AE6;"Function</h3> | ||
| + | <p style="width: 100%; margin: auto; font-size: 16px;">The ChromQ Light Chamber is a 3D-printed imaging measurement system used to quantify results of nutrient deficiency. For our current project, it is also used to quickly and inexpensively measure relative protein degradation through quantification of the color in chromoprotein expression. | ||
| + | </p></center> | ||
| + | <br> | ||
| + | <center><h3 style="color: #D49AE6;">Color Quantification</h3></center> | ||
| + | <p style="width: 100%; margin: auto; font-size: 16px;">Noora's Information Here</p> | ||
| + | <br> | ||
| + | <center><h3 style="color: #D49AE6;">Laboratory Controls</h3></center> | ||
| + | <p style="width: 100%; margin: auto; font-size: 16px;">The two controls used for imaging will be the natural color of cells and solid black. The natural color of bacterial (E.coli) cells is not simply white, but is more of a creamy-white color. Degradation at the cream-white color is 0% degradation, while solid black is 100% degradation. A third control, ideally a shade of purple with 50% degradation, will be established. | ||
| + | <br> | ||
| + | Additionally, a color-control palette will be used for consistent imaging. As we realize that smartphone cameras tend to adjust color intensity, saturation, and brightness on its own in response to the colors present, the color-controls must always be present during imaging to balance the camera’s automatic adjustments. | ||
| + | </p> | ||
| + | <br> | ||
| − | + | <center><img src="https://static.igem.org/mediawiki/2016/b/b6/T--LambertGA--camera3.jpg" style="width:18%; margin:auto;"> </center> <br> | |
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Revision as of 16:03, 16 October 2016
Measurement
The ChromQ Light Chamber is a 3D-printed imaging measurement system used to quantify results of nutrient deficiency. For our current project, it is also used to quickly and inexpensively measure relative protein degradation through quantification of the color in chromoprotein expression.
Color Quantification
Noora's Information Here
Laboratory Controls
The two controls used for imaging will be the natural color of cells and solid black. The natural color of bacterial (E.coli) cells is not simply white, but is more of a creamy-white color. Degradation at the cream-white color is 0% degradation, while solid black is 100% degradation. A third control, ideally a shade of purple with 50% degradation, will be established.
Additionally, a color-control palette will be used for consistent imaging. As we realize that smartphone cameras tend to adjust color intensity, saturation, and brightness on its own in response to the colors present, the color-controls must always be present during imaging to balance the camera’s automatic adjustments.
