Difference between revisions of "Team:NCKU Tainan/Collaborations"
| Line 3: | Line 3: | ||
</head> | </head> | ||
<body> | <body> | ||
| − | <style>@font-face { font-family: 'NotoSansCJKtc-Regular'; src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div class="container-fluid" style="width:72%"> <div class="navbar-header"> <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button> <a class="navbar-brand" href="/Team:NCKU_Tainan"> <h1>NCKU</h1><h4>Tainan</h4> </a> </div> <div id="navbar" class="navbar-collapse collapse"> <ul class="nav navbar-nav"> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu2"> <li><a href="/Team:NCKU_Tainan/Project"> | + | <style>@font-face { font-family: 'NotoSansCJKtc-Regular'; src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div class="container-fluid" style="width:72%"> <div class="navbar-header"> <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button> <a class="navbar-brand" href="/Team:NCKU_Tainan"> <h1>NCKU</h1><h4>Tainan</h4> </a> </div> <div id="navbar" class="navbar-collapse collapse"> <ul class="nav navbar-nav"> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu2"> <li><a href="/Team:NCKU_Tainan/Project">Background</a></li> <li><a href="/Team:NCKU_Tainan/Description">Description</a></li> <li><a href="/Team:NCKU_Tainan/Results">Results</a></li> <li><a href="/Team:NCKU_Tainan/Model">Modeling</a></li> <li><a href="/Team:NCKU_Tainan/Parts">Parts</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu2" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Device</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu2"> <li><a href="/Team:NCKU_Tainan/Hardware">Hardware</a></li> <li><a href="/Team:NCKU_Tainan/Software">Software</a></li> <li><a href="/Team:NCKU_Tainan/Demonstrate">Demonstrate</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu3" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Judging</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu3"> <li><a href="/Team:NCKU_Tainan/Medal">Medal</a></li> <li><a href="/Team:NCKU_Tainan/Safety">Safety</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu4" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Team">Team</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu4"> <li><a href="/Team:NCKU_Tainan/Team">Team</a></li> <li><a href="/Team:NCKU_Tainan/Attributions">Attributions</a></li> <li><a href="/Team:NCKU_Tainan/Acknowledgement">Acknowledgement</a></li> <li><a href="/Team:NCKU_Tainan/Collaborations">Collaborations</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu5" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Human_Practices">Human Practices</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu5"> <li><a href="/Team:NCKU_Tainan/Human_Practices">Overview</a></li> <li><a href="/Team:NCKU_Tainan/Integrated_Practices">Integrated Practices</a></li> <li><a href="/Team:NCKU_Tainan/Engagement">Engagement</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu6" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Notebook</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu6"> <li><a href="/Team:NCKU_Tainan/Notebook_Construction">Construction</a></li> <li><a href="/Team:NCKU_Tainan/Notebook_Functional_Test">Functional Test</a></li> <li><a href="/Team:NCKU_Tainan/Notebook_Device_Design">Device Design</a></li> <li><a href="/Team:NCKU_Tainan/Notebook_Protocols">Protocols</a></li> <li><a href="/Team:NCKU_Tainan/Notebook_Model">Model</a></li> </ul> </li> </ul> <ul class="nav navbar-nav navbar-right"> </ul> </div><!--/.nav-collapse --></div><!--/.container-fluid --></nav><div id="container-big"><div id="iGEMbar"></div><div id="line-left"></div><div id="line-left2"></div><div id="line-right"></div><div id="photo-left"></div></div><!--/.container-big --> <style> |
| − | #photo-left { background-image: url("/wiki/images/ | + | #photo-left { background-image: url("/wiki/images/0/06/T--NCKU_Tainan--Collaborations.png"); } |
</style> | </style> | ||
<div class="container-fluid" style="margin-top:100px"> | <div class="container-fluid" style="margin-top:100px"> | ||
| Line 11: | Line 11: | ||
<div class="col-md-9"> | <div class="col-md-9"> | ||
<div class="head2">Team - Collaborations</div> | <div class="head2">Team - Collaborations</div> | ||
| − | <div class="title-line" id="section1">Protocols & | + | <div class="title-line" id="section1">Protocols & Materials</div> |
| − | <div class="intro"> | + | <div class="intro">To collaborate with <a href="https://2016.igem.org/Team:TCU_Taiwan" target="_blank">TCU_Taiwan</a>, we shared the protocol and sent the material for 1000ml of Glucose-free modified M9 medium for cell culturing and functional experiments.</div> |
<h5>Protocol for modified M9:</h5> | <h5>Protocol for modified M9:</h5> | ||
<p>For preparing 10X M9 stock | <p>For preparing 10X M9 stock | ||
| Line 22: | Line 22: | ||
<li>Final pH (2X solution): 6.6–7.0 at 25°C</li> | <li>Final pH (2X solution): 6.6–7.0 at 25°C</li> | ||
</ul><p></p> | </ul><p></p> | ||
| − | <p>After | + | <p>After diluting to 1X and autoclave, for every 20 ml, add |
</p><ul><li>20% D-Glucose solution 400ul</li> | </p><ul><li>20% D-Glucose solution 400ul</li> | ||
<li>1.0 M MgSO4 solution 40ul</li> | <li>1.0 M MgSO4 solution 40ul</li> | ||
| Line 28: | Line 28: | ||
<li>20% Casamino acid 200ul</li> | <li>20% Casamino acid 200ul</li> | ||
</ul><p></p> | </ul><p></p> | ||
| − | <p> | + | <p>Modified M9 Sample sent to <a href="https://2016.igem.org/Team:TCU_Taiwan" target="_blank">TCU_Taiwan</a></p> |
<img src="/wiki/images/d/d8/T--NCKU_Tainan--collaborations1.jpg"> | <img src="/wiki/images/d/d8/T--NCKU_Tainan--collaborations1.jpg"> | ||
| − | <div class="title-line" id="section2"> | + | <div class="title-line" id="section2">Dry Lab</div> |
<h5>Collaboration with <a href="https://2016.igem.org/Team:TCU_Taiwan" target="_blank">TCU_Taiwan</a></h5> | <h5>Collaboration with <a href="https://2016.igem.org/Team:TCU_Taiwan" target="_blank">TCU_Taiwan</a></h5> | ||
| − | <p>Both of us are doing the project about glucose detection | + | <p>Both of us are doing the project about glucose detection. We discuss some issues about how <i>E.coli</i> reacts when glucose is added and shares the information and difficulties.</p> |
| − | <p> | + | <p>Furthermore, we also provide some mathematical support to derive the formula of production rates when system reaches its equilibrium.</p> |
<img src="/wiki/images/8/8f/T--NCKU_Tainan--collaborations2.jpg"> | <img src="/wiki/images/8/8f/T--NCKU_Tainan--collaborations2.jpg"> | ||
</div> | </div> | ||
| Line 39: | Line 39: | ||
<ul id="sidemenu" style="width:90%"> | <ul id="sidemenu" style="width:90%"> | ||
<li><a href="#" onclick="return toEvent('section1');">Protocols & Materials</a></li> | <li><a href="#" onclick="return toEvent('section1');">Protocols & Materials</a></li> | ||
| − | <li><a href="#" onclick="return toEvent('section2');"> | + | <li><a href="#" onclick="return toEvent('section2');">Dry Lab</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> <!-- /.container-fluid --> | </div> <!-- /.container-fluid --> | ||
| − | <script src="/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--jquery-1_12_0_min_js?ctype=text/javascript&action=raw"></script><script src="/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--bootstrap_min_js?ctype=text/javascript&action=raw"></script><script src="/Team:NCKU_Tainan/js/T--NCKU_Tainan--MathjaxConfigIgem_js?ctype=text/javascript&action=raw | + | <script src="/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--jquery-1_12_0_min_js?ctype=text/javascript&action=raw"></script><script src="/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--bootstrap_min_js?ctype=text/javascript&action=raw"></script><script src="/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><script src="/Team:NCKU_Tainan/js/T--NCKU_Tainan--MathjaxConfigIgem_js?ctype=text/javascript&action=raw"></script><script src="/Team:NCKU_Tainan/js/T--NCKU_Tainan--common_js?ctype=text/javascript&action=raw"></script><script>(function() { /* change icon */ var link = document.createElement('link'); link.type = 'image/x-icon'; link.rel = 'shortcut icon'; link.href = '/wiki/images/8/80/T--NCKU_Tainan--favicon.png'; document.getElementsByTagName('head')[0].appendChild(link);}());</script> |
</body></html> | </body></html> | ||
Revision as of 01:08, 18 October 2016
TEAM / Collaborations
Team - Collaborations
Protocols & Materials
To collaborate with TCU_Taiwan, we shared the protocol and sent the material for 1000ml of Glucose-free modified M9 medium for cell culturing and functional experiments.
Protocol for modified M9:
For preparing 10X M9 stock
- Formulation
- Disodium Phosphate Heptahydrate 128 g/L
- Monopotassium Phosphate 30 g/L
- Sodium Chloride 5 g/L
- Ammonium Chloride 10 g/L
- Final pH (2X solution): 6.6–7.0 at 25°C
After diluting to 1X and autoclave, for every 20 ml, add
- 20% D-Glucose solution 400ul
- 1.0 M MgSO4 solution 40ul
- 1.0 M CaCl2 solution 2ul
- 20% Casamino acid 200ul
Modified M9 Sample sent to TCU_Taiwan
Dry Lab
Collaboration with TCU_Taiwan
Both of us are doing the project about glucose detection. We discuss some issues about how E.coli reacts when glucose is added and shares the information and difficulties.
Furthermore, we also provide some mathematical support to derive the formula of production rates when system reaches its equilibrium.
