Difference between revisions of "Team:NCKU Tainan/Safety"
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| − | <style>@font-face { font-family: 'NotoSansCJKtc-Regular'; src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div class="container-fluid" style="width:72%"> <div class="navbar-header"> <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button> <a class="navbar-brand" href="/Team:NCKU_Tainan"> <h1>NCKU</h1><h4>Tainan</h4> </a> </div> <div id="navbar" class="navbar-collapse collapse"> <ul class="nav navbar-nav"> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu2"> <li><a href="/Team:NCKU_Tainan/Project"> | + | <style>@font-face { font-family: 'NotoSansCJKtc-Regular'; src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div class="container-fluid" style="width:72%"> <div class="navbar-header"> <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button> <a class="navbar-brand" href="/Team:NCKU_Tainan"> <h1>NCKU</h1><h4>Tainan</h4> </a> </div> <div id="navbar" class="navbar-collapse collapse"> <ul class="nav navbar-nav"> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu2"> <li><a href="/Team:NCKU_Tainan/Project">Background</a></li> <li><a href="/Team:NCKU_Tainan/Description">Description</a></li> <li><a href="/Team:NCKU_Tainan/Results">Results</a></li> <li><a href="/Team:NCKU_Tainan/Model">Modeling</a></li> <li><a href="/Team:NCKU_Tainan/Parts">Parts</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu2" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Device</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu2"> <li><a href="/Team:NCKU_Tainan/Hardware">Hardware</a></li> <li><a href="/Team:NCKU_Tainan/Software">Software</a></li> <li><a href="/Team:NCKU_Tainan/Demonstrate">Demonstrate</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu3" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Judging</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu3"> <li><a href="/Team:NCKU_Tainan/Medal">Medal</a></li> <li><a href="/Team:NCKU_Tainan/Safety">Safety</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu4" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Team">Team</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu4"> <li><a href="/Team:NCKU_Tainan/Team">Team</a></li> <li><a href="/Team:NCKU_Tainan/Attributions">Attributions</a></li> <li><a href="/Team:NCKU_Tainan/Acknowledgement">Acknowledgement</a></li> <li><a href="/Team:NCKU_Tainan/Collaborations">Collaborations</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu5" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Human_Practices">Human Practices</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu5"> <li><a href="/Team:NCKU_Tainan/Human_Practices">Overview</a></li> <li><a href="/Team:NCKU_Tainan/Integrated_Practices">Integrated Practices</a></li> <li><a href="/Team:NCKU_Tainan/Engagement">Engagement</a></li> </ul> </li> <li> <a class="dropdown-toggle" type="button" id="dropdownMenu6" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Notebook</a><div class="nav-underline"></div> <ul class="dropdown-menu" aria-labelledby="dropdownMenu6"> <li><a href="/Team:NCKU_Tainan/Notebook_Construction">Construction</a></li> <li><a href="/Team:NCKU_Tainan/Notebook_Functional_Test">Functional Test</a></li> <li><a href="/Team:NCKU_Tainan/Notebook_Device_Design">Device Design</a></li> <li><a href="/Team:NCKU_Tainan/Notebook_Protocols">Protocols</a></li> <li><a href="/Team:NCKU_Tainan/Notebook_Model">Model</a></li> </ul> </li> </ul> <ul class="nav navbar-nav navbar-right"> </ul> </div><!--/.nav-collapse --></div><!--/.container-fluid --></nav><div id="container-big"><div id="iGEMbar"></div><div id="line-left"></div><div id="line-left2"></div><div id="line-right"></div><div id="photo-left"></div></div><!--/.container-big --> <style> |
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<div class="title-line" id="plan">Safety Plan</div> | <div class="title-line" id="plan">Safety Plan</div> | ||
| − | <p>Our <i>E.coli</i> can sense urea glucose. When the glucose level is high, the bacteria can express red fluorescent protein (RFP) as a reporter to give a signal which can be detected using excitation. After the detection, the “suicide-gene inducers”, arabinose, induce the | + | <p>Our <i>E.coli</i> can sense urea glucose. When the glucose level is high, the bacteria can express red fluorescent protein (RFP) as a reporter to give a signal which can be detected using excitation. After the detection, the “suicide-gene inducers”, arabinose, induce the P<sub>BAD</sub> promoter (<a href="http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a>) and then activate the transcription of the lysis gene (<a href="http://parts.igem.org/Part:BBa_K117000">BBa_K117000</a>). The functional assays are showed in <a href="/Team:NCKU_Tainan/Results#safety">Bio-Safety System</a>.</p> |
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<div class="title-line" id="lab">Safe Lab</div> | <div class="title-line" id="lab">Safe Lab</div> | ||
| − | <h5> | + | <h5>Building a safe lab</h5> |
| − | <p>We operated the project in the Biosafety Level 1 (BSL-1) Lab. The space is suitable for experiments in all of our chassis which are Risk Group 1 organisms. Additionally, we always handled biological materials in laminar flow hood. All of biowastes were autoclaved at the typical 121 °C/15 psi for 15 minutes. </p> | + | <p>We operated the project in the Biosafety Level 1 (BSL-1) Lab. The space is suitable for experiments in all of our chassis which are Risk Group 1 organisms. Additionally, we always handled biological materials in a laminar flow hood. All of biowastes were autoclaved at the typical 121 °C/15 psi for 15 minutes.</p> |
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<div class="title-line" id="law">Laws and Regulations</div> | <div class="title-line" id="law">Laws and Regulations</div> | ||
<h5>Obeying the laws and regulations of biosafety in our Country/ University</h5> | <h5>Obeying the laws and regulations of biosafety in our Country/ University</h5> | ||
| − | <p> | + | <p>Regarding the Environmental Protection, Safety and Health Center is responsible for the safety of biology labs at National Cheng Kung University (<a href="/wiki/images/6/6c/T--NCKU_Tainan--ref3.pdf" target="_blank">Guidelines for Laboratory Biosafety</a>). And Centers for Disease Control (CDC) is the department to govern biosafety for research laboratories in our country (<a href="/wiki/images/a/a1/T--NCKU_Tainan--ref4.pdf" target="_blank">Laboratory Biosafety Management: A Compilation of Regulations and Administrative Guidance</a>).</p> |
<div class="title-line" id="shipment">Safe Shipment</div> | <div class="title-line" id="shipment">Safe Shipment</div> | ||
TODO: 中文字要改掉 | TODO: 中文字要改掉 | ||
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Revision as of 01:39, 18 October 2016
JUDGING / Safety
Safety
Safety Issues
How did we address the safety issues in our project?
- A non-pathogenic chassis, i.e. E.coli DH5α, E.coli BL21 (DE3), is chosen.
- The chassis is in small enclosed space as a barrier to prevent contamination.
- "Suicide-gene inducers" in the enclosed space are designed to kill E.coli in the bio-sensing strip.
Safety Plan
Our E.coli can sense urea glucose. When the glucose level is high, the bacteria can express red fluorescent protein (RFP) as a reporter to give a signal which can be detected using excitation. After the detection, the “suicide-gene inducers”, arabinose, induce the PBAD promoter (BBa_K206000) and then activate the transcription of the lysis gene (BBa_K117000). The functional assays are showed in Bio-Safety System.
Safe Lab
Building a safe lab
We operated the project in the Biosafety Level 1 (BSL-1) Lab. The space is suitable for experiments in all of our chassis which are Risk Group 1 organisms. Additionally, we always handled biological materials in a laminar flow hood. All of biowastes were autoclaved at the typical 121 °C/15 psi for 15 minutes.
Laws and Regulations
Obeying the laws and regulations of biosafety in our Country/ University
Regarding the Environmental Protection, Safety and Health Center is responsible for the safety of biology labs at National Cheng Kung University (Guidelines for Laboratory Biosafety). And Centers for Disease Control (CDC) is the department to govern biosafety for research laboratories in our country (Laboratory Biosafety Management: A Compilation of Regulations and Administrative Guidance).
Safe Shipment
TODO: 中文字要改掉
Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems? 寄送貨運公司? 注意事項? 是否需要準備申請文件之類的前置作業?
