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Revision as of 22:00, 18 October 2016



Best Composite Part

This collection is used to degrade 3-PBA thoroughly.

Figure 1.The sketch map of 3-PBA biodegradation process.

SectionⅠ pba gene cluster

pba pbaA1A2 1.pbaA1A2(BBa_K2088003);
2.nativeRBS-pbaA1A2B+dT(BBa_K2088016)
pbaB 3.pbaB(BBa_K2088007)
pbaC 4.pbaC+dT(BBa_K2088005);
5.B0030+UTR-pbaC (BBa_K2088668);
6.B0032+UTR-pbaC(BBa_K2088669);
7.J23100+B0034+ pbaC+dT(BBa_K2088017);
8.J23100+B0030+ pbaC+dT(BBa_K2088018)

SectionⅡ mhb gene cluster

mhb 9.mhbT(BBa_K2088666)
10.copA+riboJ+mhbDHIM+dT(BBa_K2088672)

Section Ⅲ C230

C23O 11.C23O(BBa_K2088000)
12.nativeRBS-C23O(BBa_K2088008)
13.nativeRBS-C23O+dT(BBa_K2088009)

Section Ⅳ Regulatory factors

mhb operon mhbR 14.mhbR(BBa_K2088011);
15.optimized mhbR(BBa_K2088670)
mhbPt 16.mhbPt(BBa_K2088013);
17.mhbPt+GFP(BBa_K2088004);
18.mhbPt(left)+dT(BBa_K2088612);
19.mhbPt(left)+GFP(BBa_K2088689)
mhbR+mhbPt 20.mhbR+mhbPt(BBa_K2088012);
21.dT+mhbR+mhbPt+GFP(BBa_K2088002);
22.J23100+B0034+mhbT+dT(BBa_K2088671)+dT+mhbR+mhbPt+GFP(BBa_K2088616)
copA 23.copA(BBa_K2088001)
J23101+riboJ+M6+GFP+dT 24.J23101+riboJ+M6+GFP+dT(BBa_K2088014)