Difference between revisions of "Team:LambertGA/Results"

Line 374: Line 374:
 
6. Pictures:
 
6. Pictures:
 
<br>
 
<br>
−
<img src"https://static.igem.org/mediawiki/2016/e/e8/T--LambertGA--platereaderresults.jpg">
+
<img src="https://static.igem.org/mediawiki/2016/e/e8/T--LambertGA--platereaderresults.jpg" style="width:34%; margin:auto;">
 
<br>
 
<br>
 +
<center><i> Data from the Plate Reader </i></center>
 
</p>
 
</p>
−
<br>
+
<img src="https://static.igem.org/mediawiki/2016/e/e8/T--LambertGA--platereaderresults.jpg" style="width:34%; margin:auto;">
−
<center><h3 style="color: #D49AE6;">Relative Color Quantification</h3></center>
+
−
<p style="width: 100%; margin: auto; font-size: 16px;">Relative strength of the degradation of tsPurple cells (control, TS Purple DAS, and TS Purple LAA) can be measured by using a smartphone camera lens or any simple photography device (along with the color-controls for consistency).
+
−
<br><br>
+
−
 
+
  
  

Revision as of 04:16, 19 October 2016

Inoculations of GFP Constructs (58 tubes)

After making our constructs, we inoculated cultures from previous transformations that have successfully expressed the fluorescent protein.
1.P-Lambda-R--LacI--GFP (no deg tag)

  1. 3 tubes in each cell type (DH10, Keio Wild, Keio ClpP) induced with 0 uM IPTG (plain LB)
  2. 3 tubes of each cell type (DH10, Keio Wild, Keio ClpP) induced with 100 uM IPTG (plain LB)
  3. RESULTS:
2. P-Lambda-R--LacI--GFP (DAS)
  1. 3 tubes in each cell type (DH10, Keio Wild, Keio ClpP) induced with 0 uM IPTG (plain LB)
  2. 3 tubes of each cell type (DH10, Keio Wild, Keio ClpP) induced with 100 uM IPTG (plain LB)
  3. RESULTS:
3. P-Lambda-R--LacI--GFP (LAA)
  1. 3 tubes in each cell type (DH10, Keio Wild, Keio ClpP) induced with 0 uM IPTG (plain LB)
  2. 3 tubes of each cell type (DH10, Keio Wild, Keio ClpP) induced with 100 uM IPTG (plain LB)
  3. RESULTS:
4. Plain LB, DH10 cells in plain LB, Keio Wild cells in plain LB, and Keio ClpP cells in plain LB 5. RESULTS:
  1. 10/17: The GFP constructs were brought to the plate reader at Georgia Tech. Although cells were grown in the liquid culture, they did not fluoresce.
6. Pictures:

Data from the Plate Reader