Difference between revisions of "Team:NAU-CHINA/Basic Part"
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<h2><a href="http://parts.igem.org/Part:BBa_K2088000"><b style="color: blue;">BBa_K2088000</b></a></h2> | <h2><a href="http://parts.igem.org/Part:BBa_K2088000"><b style="color: blue;">BBa_K2088000</b></a></h2> | ||
<p style="font-family:'Comic Sans MS','Arial Black';color: rgb(100,100,100);font-size:17px;">This basic part encodes Catechol 2,3-Dioxygenase from Sphingobium sp. YBL2. This protein transforms catechol by meta-cleavage to the bright yellow product 2-hydroxymuconate semialdehyde(2-HMS). We tested it with J23100 (a strong promoter) and its native rbs before it and a double terminator after it in E.coli. It plays a great role in our project to degrade catechol to 2-HMS, a non-toxic molecule that can be metalbolized by E.coli DH5α.</p> | <p style="font-family:'Comic Sans MS','Arial Black';color: rgb(100,100,100);font-size:17px;">This basic part encodes Catechol 2,3-Dioxygenase from Sphingobium sp. YBL2. This protein transforms catechol by meta-cleavage to the bright yellow product 2-hydroxymuconate semialdehyde(2-HMS). We tested it with J23100 (a strong promoter) and its native rbs before it and a double terminator after it in E.coli. It plays a great role in our project to degrade catechol to 2-HMS, a non-toxic molecule that can be metalbolized by E.coli DH5α.</p> | ||
| − | <p><a href=""><b style="color: blue;">For more details, see our experiment of C23O.</b></a></p> | + | <p><a href="https://2016.igem.org/Team:NAU-CHINA/Experiment/CAT"><b style="color: blue;">For more details, see our experiment of C23O.</b></a></p> |
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Revision as of 04:22, 19 October 2016
Best Basic Part
BBa_K2088000
This basic part encodes Catechol 2,3-Dioxygenase from Sphingobium sp. YBL2. This protein transforms catechol by meta-cleavage to the bright yellow product 2-hydroxymuconate semialdehyde(2-HMS). We tested it with J23100 (a strong promoter) and its native rbs before it and a double terminator after it in E.coli. It plays a great role in our project to degrade catechol to 2-HMS, a non-toxic molecule that can be metalbolized by E.coli DH5α.
For more details, see our experiment of C23O.
