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Revision as of 15:14, 19 October 2016


Proof Of Concept


Prior Work


These results were obtained using overnight cultures grown at 37C in a shaking incubator at 170rpm. The values were normalized using control tubes of LB and Keio ClpP cells grown under the same conditions. The strains that were grown were the full “Switch” constructs with GFP DAS and GFP LAA degradation tags. Unfortunately, the control cells of GFP without a tag did not grow. The initial results seem to indicate that under induction of IPTG the level of GFP protein does indeed decrease. Further experimentation will be conducted to include a control construct without a degradation tag as well as a comparison to Keio Wild type strain.