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| <p>The composite parts in our project were all assembled in a similar fashion, creating two new BioBricks for each protein. The first construct was generally a simple assembly with a T7 promoter allowing for inducible protein expression. The second BioBrick contained an additional sequence consisting or a linker and two tags (LT) - one needed for purification and one for Sortase-A-mediated conjugation of our proteins to spider silk.</p> | | <p>The composite parts in our project were all assembled in a similar fashion, creating two new BioBricks for each protein. The first construct was generally a simple assembly with a T7 promoter allowing for inducible protein expression. The second BioBrick contained an additional sequence consisting or a linker and two tags (LT) - one needed for purification and one for Sortase-A-mediated conjugation of our proteins to spider silk.</p> |
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− | <p><a href="http://parts.igem.org/Part:BBa_K2144000">BBa_K2144000</a> T7-Nuclease<br> | + | <p><a href="http://parts.igem.org/Part:BBa_K2144000" target="_blank">BBa_K2144000</a> T7-Nuclease <br> |
− | <a href="http://parts.igem.org/Part:BBa_K2144011">BBa_K2144011</a> T7-Nuclease-LT<br> | + | <a href="http://parts.igem.org/Part:BBa_K2144011" target="_blank">BBa_K2144011</a> T7-Nuclease-LT</p> |
− | Nuc2, isolated form <em>S.epidermidis</em> is a well-known nuclease that we assembled with a T7 promoter in order to degrade biofilm-associated extracellular DNA.</p>
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− | <p><a href="http://parts.igem.org/Part:BBa_K2144001">BBa_K2144001</a> T7-Lysostaphin<br> | + | <p>Nuc2, isolated form <em>S.epidermidis</em> is a well-known nuclease that we assembled with a T7 promoter in order to degrade biofilm-associated extracellular DNA.</p> |
− | <a href="http://parts.igem.org/Part:BBa_K2144005">BBa_K2144005</a> T7-Lysostaphin-LT<br> | + | |
− | Lysostaphin displays a highly specific endopeptidase activity which has been shown to degrade a key components of many biofilms as well as peptidoglycans found in cell walls. </p> | + | <p><a href="http://parts.igem.org/Part:BBa_K2144001" target="_blank">BBa_K2144001</a> T7-Lysostaphin <br> |
| + | <a href="http://parts.igem.org/Part:BBa_K2144005" target="_blank">BBa_K2144005</a> T7-Lysostaphin-LT</p> |
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| + | <p>Lysostaphin displays a highly specific endopeptidase activity which has been shown to degrade a key components of many biofilms as well as peptidoglycans found in cell walls. </p> |
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| <p><a href="http://parts.igem.org/Part:BBa_K2144010">BBa_K2144010</a> T7-Esp<br> | | <p><a href="http://parts.igem.org/Part:BBa_K2144010">BBa_K2144010</a> T7-Esp<br> |
− | <a href="http://parts.igem.org/Part:BBa_K2144012">BBa_K2144012</a> T7-Esp-LT<br> | + | <a href="http://parts.igem.org/Part:BBa_K2144012">BBa_K2144012</a> T7-Esp-LT<br></p> |
− | Esp is a serine protease secreted by <em>S.epidermidis</em> which degrades a variety of cell wall- and biofilm-associated proteins, among them many virulence factors.</p>
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− | <p><a href="http://parts.igem.org/Part:BBa_K2144003">BBa_K2144003</a> T7-Defensin<br> | + | <p>Esp is a serine protease secreted by <em>S.epidermidis</em> which degrades a variety of cell wall- and biofilm-associated proteins, among them many virulence factors.</p> |
− | <a href="http://parts.igem.org/Part:BBa_K2144007">BBa_K2144007</a> T7-Defensin-LT<br> | + | |
− | Defensins are a class of molecules known to disrupt membrane integrity of pathogenic organisms, dramatically increasing membrane permeability and leading to cell death.</p> | + | <p><a href="http://parts.igem.org/Part:BBa_K2144003" target="_blank">BBa_K2144003</a> T7-Defensin <br> |
| + | <a href="http://parts.igem.org/Part:BBa_K2144007" target="_blank">BBa_K2144007</a> T7-Defensin-LT</p> |
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| + | <p>Defensins are a class of molecules known to disrupt membrane integrity of pathogenic organisms, dramatically increasing membrane permeability and leading to cell death.</p> |
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| <h6 id="basicpartsbr">Basic Parts<br></h6> | | <h6 id="basicpartsbr">Basic Parts<br></h6> |
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| <p>The basic part we worked on was Sortase A; a transpeptidase commonly used in biotechnology for the conjugation of peptides. The Sortase A Biobrick has it's own page - go and <a href="https://2016.igem.org/Team:Stockholm/Basic_Parts">have a look!</a> <br> | | <p>The basic part we worked on was Sortase A; a transpeptidase commonly used in biotechnology for the conjugation of peptides. The Sortase A Biobrick has it's own page - go and <a href="https://2016.igem.org/Team:Stockholm/Basic_Parts">have a look!</a> <br> |
− | <a href="http://parts.igem.org/Part:BBa_K2144008">BBa_K2144008</a> Sortase A</p> | + | <a href="http://parts.igem.org/Part:BBa_K2144008" target="_blank">BBa_K2144008</a> Sortase A</p> |
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| <h6 id="partcollectionthesortasepackagebr">Part Collection - The Sortase Package<br></h6> | | <h6 id="partcollectionthesortasepackagebr">Part Collection - The Sortase Package<br></h6> |
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| <p>Sortase A alone is a useful tool but we aimed to provide an entire toolbox, containing the Sortase A BioBrick as well as a vector for simple addition of Sortase tags to any protein and user-friendly instructions for the assembly of proteins using this toolbox. More information about the package can be found <a href="https://2016.igem.org/Team:Stockholm/Part_Collection">here</a>. <br> | | <p>Sortase A alone is a useful tool but we aimed to provide an entire toolbox, containing the Sortase A BioBrick as well as a vector for simple addition of Sortase tags to any protein and user-friendly instructions for the assembly of proteins using this toolbox. More information about the package can be found <a href="https://2016.igem.org/Team:Stockholm/Part_Collection">here</a>. <br> |
− | <a href="http://parts.igem.org/Part:BBa_K2144008">BBa_K2144008</a> Sortase A<br> | + | <a href="http://parts.igem.org/Part:BBa_K2144008" target="_blank">BBa_K2144008</a> Sortase A <br> |
− | <a href="http://parts.igem.org/Part:BBa_K2144101">BBa_K2144101</a> LT</p> | + | <a href="http://parts.igem.org/Part:BBa_K2144101" target="_blank">BBa_K2144101</a> LT</p> |
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− | <hr>
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| </section> | | </section> |
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| </article> | | </article> |
| </main></div></html>{{Template:Stockholm/Footer}} | | </main></div></html>{{Template:Stockholm/Footer}} |