Team:Stanford-Brown/SB16 Notebooks AptamerPurification


Stanford-Brown 2016

Aptamer Purification (DNAzyme, PQQ, SELEX) · Benchling

Aptamer Purification (DNAzyme, PQQ, SELEX)

Made with Benchling
Project: iGEM 2016
Authors: Michael Becich, Amy Weissenbach, Charles Gleason, Julia Gross
Dates: 2016-08-29 to 2016-08-31
Monday, 8/29
Made B&W Buffer
Tuesday, 8/30
Made 1M Lysine-HCl Stock
Wednesday, 8/31
Morning with Google X Project Loon
Made 2x Binding Buffer (100mM HEPES, 200mM KCl, 50mM MgCl2, 10mM CaCl2)
Made 1M CuSO4 stock
Washed Magnetic Streptavidin Dynabeads
Immobilized Biotinylated Oligonucleotides on beads
Incubated overnight