Team:MIT/Repressor Group Experiment 1 sequencing
Experiment 1 plasmid verification by sequencing
Made with Benchling
Project: miRNA and Repressors subgroup
Authors: Maya Kaul
Date: 2016-06-07
Tuesday, 6/7/16
A | B | |
1 | Tube | Content |
2 | 1 | hef1a:rtTA - F |
3 | 2 | hef1a:rtTA - R |
4 | 3 | Tal14:EYFP - F |
5 | 4 | Tal14:EYFP - R |
6 | 5 | TRE: Tal14 - F |
7 | 6 | TRE: Tal14 - R |
Table1
Sequencing BM3R1:meYFP, TRE:BM3R1, TRE:Tal21
Made with Benchling
Project: miRNA and Repressors subgroup
Authors: Gizem Gumuskaya
Date: 2016-06-27
Monday, 6/27
A | B | |
1 | BM3R1:meYFP 2 | Forward |
2 | BM3R1:meYFP 2 | Reverse |
3 | TRE:BM3R1 1 | Forward |
4 | TRE:BM3R1 1 | Reverse |
5 | TRE:BM3R1 2 | Forward |
6 | TRE:BM3R1 2 | Reverse |
7 | TRE:Tal21 1 | Forward |
8 | TRE:Tal21 1 | Reverse |
9 | TRE:Tal21 2 | Forward |
10 | TRE:Tal21 2 | Reverse |
Table1
Each tube contains:
10 ul of 50ng/ul DNA construct
5 ul of Forward or Reverse pDEST sequencing primers
Sequencing Tal21:EYFP and TRE:Tal21
Made with Benchling
Project: miRNA and Repressors subgroup
Authors: Wangui Mbuguiro
Date: 2016-07-13
Wednesday, 7/13
A | B | C | D | E | |
1 | Tube | Name | Primer | Length | |
2 | 5 | Tal21:EYFP_fwd | bt01 | 3862 | |
3 | 6 | Tal21:EYFP_rev | bt02 | 3862 | |
4 | 7 | Tre:tal21_f | bt01 | 6536 | |
5 | 8 | Tre:tal21_r | bt02 | 6536 |
Table1
