Team:MIT/Experiments

• BACKGROUND
• EXPERIMENTS
  Synthetic Promoters microRNA Recombinase Proof of Concept Collaborations
• FUTURE WORK
  Circuit Design Clinical Applications
• HUMAN PRACTICES
  Parts Toolbox Accessible Protocols Integrated Human Practices Public Outreach Interlab Study
• NOTEBOOK
  Lab Notebook Parts Mammalian Parts Collection Attributions Safety
• AWARDS
  HP Silver HP Gold Integrated HP Basic Part
• TEAM

Experiments

To tackle the size of this project, we organized our team into three sub-groups,
each addressing a synthetic biological tool that could assist in the diagnosing of
endometriosis. Then we combined our efforts into a proof of concept.
We also worked with other teams in the spirit of iGEM collaboration to test our
projects in new environments.

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  Synthetic mammalian promoters designed with repeating protein binding sequences demonstrated
  significant increase in reporter activity when induced with estrogen or progesterone in hormone-sensitive cell lines.
  
  
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  Observed change in microRNA activity in an endometrial cell line under different conditions
  and characterized microRNA target site sensitivity.
  
  
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  Serine recombinase TP901 successfully activates flipped EYFP when the upstream promoter is induced
  and L7Ae/k-turn, RNA-based gene repressing system, reduces basal expression of the recombinase.
  
  
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  Read about a summary of our results and how our sensors interact logically after transfection of
  4 to 5-unit genetic circuits into model cell cultures
  
  
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  Read about how we worked with other iGEM teams throughout our project
  
  

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