Difference between revisions of "Team:NCKU Tainan/Measurement"

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    <meta charset="utf-8"><link rel="shortcut icon" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png" type="image/x-icon"><link rel="icon" type="image/png" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png"><link rel="icon" type="image/x-icon" href="/wiki/images/8/80/T--NCKU_Tainan--favicon.png"><meta name="viewport" content="width=device-width, initial-scale=1.0"><meta property="og:title" content="Project Measurement - iGEM NCKU"><meta property="og:site_name" content="Project Measurement - iGEM NCKU"><meta property="og:description" content=""><title>Project Measurement - iGEM NCKU</title><meta http-equiv="Content-Type" content="text/html" charset="utf-8"><meta property="og:image" content=""><meta property="og:image:type" content="image/png"><link rel="stylesheet" href="/Team:NCKU_Tainan/css/frame/T--NCKU_Tainan--bootstrap_min_css?ctype=text/css&amp;action=raw"><link href="/Team:NCKU_Tainan/font/T--NCKU_Tainan--NotoSans_css?ctype=text/css&amp;action=raw" rel="stylesheet" type="text/css"><link rel="stylesheet" href="/Team:NCKU_Tainan/font/T--NCKU_Tainan--font-awesome_min_css?ctype=text/css&amp;action=raw"> <link rel="stylesheet" href="/Team:NCKU_Tainan/css/T--NCKU_Tainan--Layout_css?ctype=text/css&amp;action=raw">
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    <style>@font-face { font-family: 'NotoSansCJKtc-Regular';  src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div id="navbar-container" class="container-fluid">  <div class="navbar-header">      <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false">        <span class="sr-only">Toggle navigation</span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>      </button>      <a class="navbar-brand" href="/Team:NCKU_Tainan">        <h1>NCKU</h1><h4>Tainan</h4>      </a>  </div>  <div id="navbar" class="navbar-collapse collapse">    <ul class="nav navbar-nav">      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Project">Background</a></li>          <li><a href="/Team:NCKU_Tainan/Description">Description</a></li>          <li><a href="/Team:NCKU_Tainan/Results">Results</a></li>          <li><a href="/Team:NCKU_Tainan/Model">Modeling</a></li>          <li><a href="/Team:NCKU_Tainan/Parts">Parts</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu2" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Device</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Hardware">Hardware</a></li>          <li><a href="/Team:NCKU_Tainan/Software">Software</a></li>          <li><a href="/Team:NCKU_Tainan/Demonstrate">Demonstrate</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu3" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Judging</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu3">          <li><a href="/Team:NCKU_Tainan/Medal">Medal</a></li>          <li><a href="/Team:NCKU_Tainan/Safety">Safety</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu4" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Team">Team</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu4">          <li><a href="/Team:NCKU_Tainan/Team">Team</a></li>          <li><a href="/Team:NCKU_Tainan/Attributions">Attributions</a></li>          <li><a href="/Team:NCKU_Tainan/Acknowledgement">Acknowledgement</a></li>          <li><a href="/Team:NCKU_Tainan/Collaborations">Collaborations</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu5" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Human_Practices">Human Practices</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu5">          <li><a href="/Team:NCKU_Tainan/Human_Practices">Overview</a></li>          <li><a href="/Team:NCKU_Tainan/Integrated_Practices">Integrated Practices</a></li>          <li><a href="/Team:NCKU_Tainan/Engagement">Engagement</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu6" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Notebook</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu6">          <li><a href="/Team:NCKU_Tainan/Notebook_Construction">Construction</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Functional_Test">Functional Test</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Device_Design">Device Design</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Protocols">Protocols</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Model">Model</a></li>        </ul>      </li>    </ul>    <ul class="nav navbar-nav navbar-right">    </ul>  </div><!--/.nav-collapse --></div><!--/.container-fluid --></nav><div id="container-big"><div id="iGEMbar"></div><div id="line-left"></div><div id="line-left2"></div><div id="line-right"></div><div id="photo-left"></div></div><!--/.container-big --> <style>
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<div class="container-fluid" style="margin-top:100px">
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<div class="head">PROJECT / Measurement</div>
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<div class="head2">Measurement</div>
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<div class="title-line" id="sec1">Overview</div>
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<p>One of the main aspects in our project, U-KNOW, is to measure the exact urine glucose concentration through a non-invasive and user-friendly way. By  using the fluorescent intensity of glucose-induced RFP,  we  can exam the urine glucose  successfully and precisely.</p>
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<p>The degree of fluorescent intensity has shed light on how our device functions.  We approached and analyzed it numerically by applying mathematical fitting as well as theoretically by modeling with enzyme kinetics. As a result, we derived a prediction formula that can use fluorescent intensity as an input to generate the value the glucose concentration. To verify the formula, we performed a test to determine whether U-KNOW can quantify the concentration of glucose. The result showed the average recovery rate is 104% (95% ~114%) when compared The theoretical value to the actual value with n=63, the average recovery rate is 104%.</p>
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<div class="title-line long" id="sec2"></div>
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<div class="title-content">Measurement: Prediction and Quantifying the amount of urine glucose</div>
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<p>Our goal is to determine the amount of glucose present in urine sample. Thus, we used final glucose concentration of: 0, 1, 3, 5, 15, 30, 45 mM to induce our U. coli for 12 hours in 96 well and measure the fluorescent intensity at excitation/emission wavelength 562 nm / 599 nm, the difference of fluorescent intensity between each group were significant at T (time) = 8hr.</p>
  
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          <img src="/wiki/images/7/7f/T--NCKU_Tainan--image006.png" alt="fig5 result">
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            <p><b>Figure 5:</b> Fluorescence intensity with 95% confidence interval after 8 hour induction with final glucose concentration 0, 1, 3 5, 15, 30, 45 mM, all differences between each group shows statistical significance (P&gt;0.05, student t-test), proving that out U. coli do differentiate urine sample containing various amount of glucose, ranging from 0~45 mM.</p>
  
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              <p>Based on the results above, we derived a <a href="/Team:NCKU_Tainan/Model_Fitting_Theory">prediction formula</a>, to calculate the urine glucose concentration.</p>
  
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            <img src="/wiki/images/8/89/T--NCKU_Tainan--project-modeling-fitting-image5.png" alt="fig6 result">
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            <p><b>Figure 6:</b> $$\frac{dY}{dt}=\frac{17.05\times [Glucose]^{1.18}+16.07}{[Glucose]^{1.18}+4.04}$$</p>
  
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              <p>To verify the formula, we performed a test to clarify whether our U. coli can predict and quantify the concentration of urine glucose. </p>
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<h3>★  ALERT! </h3>
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              <p>Reuslt: with n=63, the average recovery rate is 104% (95% ~114%), which proved that our U. coli do predict and quantify the concentration of urine glucose with good precision.</p>
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Awards"> Best Measurement award</a>. </p>
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              <p>In conclusion, by applying this <a href="/Team:NCKU_Tainan/Model_Fitting_Theory">prediction formula</a>, it shown that our U. coli could predict and quantify the concentration of urine glucose.</p>
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<li><a href="#" onclick="return toEvent('sec1');">Overview</a></li>
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<li><a href="#" onclick="return toEvent('sec2');">Prediction &amp; Quantifying</a></li>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<p>There are a lot of exciting Parts in the Registry, but many Parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
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<h5>Inspiration</h5>
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<p>You can look at what other teams did to get some inspiration! <br />
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Here are a few examples:</p>
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus">2014 Valencia Biocampus</a></li>
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<li><a href="https://2015.igem.org/Team:William_and_Mary">2015 William and Mary</a></li>
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Latest revision as of 16:08, 19 October 2016

Project Measurement - iGEM NCKU

PROJECT / Measurement
Measurement
Overview

One of the main aspects in our project, U-KNOW, is to measure the exact urine glucose concentration through a non-invasive and user-friendly way. By using the fluorescent intensity of glucose-induced RFP, we can exam the urine glucose successfully and precisely.

The degree of fluorescent intensity has shed light on how our device functions. We approached and analyzed it numerically by applying mathematical fitting as well as theoretically by modeling with enzyme kinetics. As a result, we derived a prediction formula that can use fluorescent intensity as an input to generate the value the glucose concentration. To verify the formula, we performed a test to determine whether U-KNOW can quantify the concentration of glucose. The result showed the average recovery rate is 104% (95% ~114%) when compared The theoretical value to the actual value with n=63, the average recovery rate is 104%.

Measurement: Prediction and Quantifying the amount of urine glucose

Our goal is to determine the amount of glucose present in urine sample. Thus, we used final glucose concentration of: 0, 1, 3, 5, 15, 30, 45 mM to induce our U. coli for 12 hours in 96 well and measure the fluorescent intensity at excitation/emission wavelength 562 nm / 599 nm, the difference of fluorescent intensity between each group were significant at T (time) = 8hr.

fig5 result

Figure 5: Fluorescence intensity with 95% confidence interval after 8 hour induction with final glucose concentration 0, 1, 3 5, 15, 30, 45 mM, all differences between each group shows statistical significance (P>0.05, student t-test), proving that out U. coli do differentiate urine sample containing various amount of glucose, ranging from 0~45 mM.

Based on the results above, we derived a prediction formula, to calculate the urine glucose concentration.

fig6 result

Figure 6: $$\frac{dY}{dt}=\frac{17.05\times [Glucose]^{1.18}+16.07}{[Glucose]^{1.18}+4.04}$$

To verify the formula, we performed a test to clarify whether our U. coli can predict and quantify the concentration of urine glucose.

Reuslt: with n=63, the average recovery rate is 104% (95% ~114%), which proved that our U. coli do predict and quantify the concentration of urine glucose with good precision.

In conclusion, by applying this prediction formula, it shown that our U. coli could predict and quantify the concentration of urine glucose.