Difference between revisions of "Team:Stanford-Brown/SB16 Notebooks Nylon"

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<title>Nylon/Styrene-Butadiene Rubber/Other Materials · Benchling</title>
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<body><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Nylon/Styrene-Butadiene Rubber/Other Materials</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> iGEM 2016</div><div><b>Authors:</b> Michael Becich, Elias Robinson</div><div><b>Dates:</b> 2016-06-29 to 2016-07-11</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4365" data-index="0" style="margin-left:0;">-Cryostocked <i>E. coli </i>strains (eAKP-71 and eAKP-744) expressing enzymes from biosynthesis pathway for 6-Aminocaproic Acid from Stefan Turk (DSM Food Specialties B.V., Netherlands) arrived to the lab</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4366" data-index="1" style="margin-left:0;">-Made four liquid cultures, half incubated at 37C at 220 rpm to stimulate rapid division and plasmid copy, and half at room temp and ~60 rpm to optimize effectiveness of oxygen sensitive chain elongation enzymes (AksD/E complex)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4367" data-index="2" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4368" data-index="3" style="margin-left:0;">-Miniprepped plasmids from <span style="color:#344043"><span style="background-color:#FFFFFF">eAKP-71 and eAKP-744 (Elias)</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4369" data-index="4" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">-Ordered primers for PCR amplification of promoters, RBSs, and operons.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4370" data-index="5" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4371" data-index="6" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4372" data-index="7" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 7/11</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4373" data-index="8" style="margin-left:35px;">Anioninc Polymerization vs. Emulison Polymeriation of Butadiene</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part4374" data-index="9" style="margin-left:35px;">Heat Ethanol to high temperature</div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div>
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Latest revision as of 19:52, 19 October 2016


Stanford-Brown 2016

Nylon/Styrene-Butadiene Rubber/Other Materials · Benchling

Nylon/Styrene-Butadiene Rubber/Other Materials

Made with Benchling
Project: iGEM 2016
Authors: Michael Becich, Elias Robinson
Dates: 2016-06-29 to 2016-07-11
Wednesday, 6/29
-Cryostocked E. coli strains (eAKP-71 and eAKP-744) expressing enzymes from biosynthesis pathway for 6-Aminocaproic Acid from Stefan Turk (DSM Food Specialties B.V., Netherlands) arrived to the lab
-Made four liquid cultures, half incubated at 37C at 220 rpm to stimulate rapid division and plasmid copy, and half at room temp and ~60 rpm to optimize effectiveness of oxygen sensitive chain elongation enzymes (AksD/E complex)
Thursday, 6/30
-Miniprepped plasmids from eAKP-71 and eAKP-744 (Elias)
-Ordered primers for PCR amplification of promoters, RBSs, and operons.
Monday, 7/11
●
Anioninc Polymerization vs. Emulison Polymeriation of Butadiene
●
Heat Ethanol to high temperature