Difference between revisions of "Team:Stanford-Brown/SB16 Notebooks Interlab"
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<li><a href="https://2016.igem.org/Team:Stanford-Brown/Integrated_Practices">Integrated Human Practices</a></li> | <li><a href="https://2016.igem.org/Team:Stanford-Brown/Integrated_Practices">Integrated Human Practices</a></li> | ||
<li><a href="https://2016.igem.org/Team:Stanford-Brown/Engagement">Outreach</a></li> | <li><a href="https://2016.igem.org/Team:Stanford-Brown/Engagement">Outreach</a></li> | ||
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| − | <li><a href="https://2016.igem.org/Team:Stanford-Brown/SB16_Practices_Exploration"> | + | <li><a href="https://2016.igem.org/Team:Stanford-Brown/SB16_Practices_Exploration">Life Beyond the Lab</a></li> |
</ul> | </ul> | ||
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<li><a href="https://2016.igem.org/Team:Stanford-Brown/Parts">BioBricks</a></li> | <li><a href="https://2016.igem.org/Team:Stanford-Brown/Parts">BioBricks</a></li> | ||
| − | <!-- | + | <!--protocols--> |
<li class="dropdown"> | <li class="dropdown"> | ||
| − | <a href="#" class="dropdown-toggle active" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> | + | <a href="#" class="dropdown-toggle active" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Protocols<span class="caret"></span></a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a href="https://2016.igem.org/Team:Stanford-Brown/ | + | <li><a href="https://2016.igem.org/Team:Stanford-Brown/SB16_Protocols">Protocols</a> |
| + | </li> | ||
| + | <li><a href="https://2016.igem.org/Team:Stanford-Brown/SB16_Software">Software Design</a> | ||
| + | </li> | ||
| + | <li><a href="https://2016.igem.org/Team:Stanford-Brown/SB16_Modeling">Modeling</a> | ||
</li> | </li> | ||
<li role="separator" class="divider"></li> | <li role="separator" class="divider"></li> | ||
Latest revision as of 19:52, 19 October 2016
iGEM (InterLab, Cell Competency Kit, BioBrick Characterizations, etc.)
Made with Benchling
Project: iGEM 2016
Authors: Taylor Sihavong, Charles Gleason, Michael Becich
Dates: 2016-06-16 to 2016-09-06
Thursday, 6/16/16
-Cell competency test done on 3 different DNA concentrations (0.5pg/ul, 10pg/ul, 50pg/ul) with 2 different cell types (T7, 5-alpha)
-Protocol given for the competency test wasn't compatible with T7, but we got colonies on all of our 5-alpha plates (Charlie, Taylor)
Monday, 6/20
-Began InterLab study. Started with the high efficiency transformation protocol for NEB 5-alpha Competent E. Coli. Transformed 5 DNA samples: positive control, negative control, and 3 test devices. (Taylor, Cynthia, Amy)
-Test device 3 was diluted with 100ul DI water with 5ul of DNA taken out
-Plated with 1:4 dilutions
Tuesday, 6/21
-InterLab study results: test device 1 plate had no colonies --> store positive control, negative control, test device 2, test device 3 at -4˚C (Charlie)
IMG_2671.JPG
-Redo transformation of test device 1 and test device 3
-Test device 1 was diluted with 100ul DI water with 5ul of DNA taken out
-2 plates each, a 4X dilution and a 40X dilution
Tuesday, 6/28
-Team Brainstorming Session: Where are we at and where are we heading?
IMG_2634.JPG
Thursday, 6/30
-Performed calibration plate readings (2 plates) for interlab study (Taylor S)
-Prepared overnight cultures for interlab study (2 colonies per plate, 5 plates) (Mike)
Friday, 7/1
-Made Terrific Broth (TB, 0.17M KH2PO4, 0.72M K2HPO4) buffer for interlab study, Prepared Interlab Samples for Weekend Storage
Tuesday, 7/5
- Worked on part 2 of Interlab Measurement Study for 6 hours, measured fluorescence after 6 hours with SpectraMax Plus 384
Wednesday, 7/20
●
37th Anniversary of Putting First Human on the Moon!
Thursday, 7/21
●
NASA Star Trek Premiere Special Showing!
●
Jim Head Presentation and Q&A (Mars Climate Change, Psychology of Family of Planets)
Friday, 7/22
Saturday, 7/23
-Hackathon to learn methods for improving website
Tuesday, 9/6
●
Freeze dried 10 uL of first 8 BioBricks!
○
1st Submission: rAIP, AE Blue, 1 np Et?, Gb2, c1, c2, c3, cw
