Difference between revisions of "Team:MIT/Parts"

 
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<h1 style="color:#FFFFFF; background-color:#F20253;; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: trebuchet MS"> Basic Parts - Entry Vectors</h1>
 
<br>
 
<br>
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<center>
 
<table>
 
<table>
 
<tr>
 
<tr>
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<tr>
 
<tr>
 
<td> pENTR rtTA</td>
 
<td> pENTR rtTA</td>
−
<td> <a href = "http://parts.igem.org/Part:BBa_K2100022">BBa_K2100022</a></td>
+
<td> <a href = "http://parts.igem.org/Part:BBa_K2100025">BBa_K2100025</a></td>
 
</tr>
 
</tr>
  
 +
<tr>
 +
<td> pENTR VgEcR</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100026">BBa_K2100026</a></td>
 +
</tr>
  
 
<tr>
 
<tr>
−
<td> pEXPR hEF1a-eYFP</td>
+
<td> pENTR attB-flipped eYFP-attP</td>
−
<td> <a href = "http://parts.igem.org/Part:BBa_K2100011">BBa_K2100011</a></td>
+
<td> <a href = "http://parts.igem.org/Part:BBa_K2100049">BBa_K2100049</a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td> pEXPR hEF1a-mKate</td>
+
<td> pENTR EGSH-2kturn</td>
−
<td> <a href = "http://parts.igem.org/Part:BBa_K2100012">BBa_K2100012</a></td>
+
<td> <a href = "http://parts.igem.org/Part:BBa_K2100050">BBa_K2100050</a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td> pEXPR hEF1a-tagBFP</td>
+
<td> pENTR EGSH-4kturn</td>
−
<td> <a href = "http://parts.igem.org/Part:BBa_K2100013">BBa_K2100013</a></td>
+
<td> <a href = "http://parts.igem.org/Part:BBa_K2100055">BBa_K2100055</a></td>
 
</tr>
 
</tr>
  
 +
<tr>
 +
<td> pENTR hEF1a-SV40</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100061">BBa_K2100061</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pENTR L7Ae</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100068">BBa_K2100068</a></td>
 +
</tr>
  
 +
</table>
 +
<br>
  
 +
<h1 style="color:#FFFFFF; background-color:#F20253;; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: trebuchet MS"> Composite Parts - Expression Vectors</h1>
 +
<br>
 +
<table>
 
<tr>
 
<tr>
−
<td> pDEST mCherry GG</td>
+
<td> pEXPR hEF1a:eYFP</td>
−
<td> <a href = "http://parts.igem.org/Part:BBa_K2100015">BBa_K2100015</a></td>
+
<td> <a href = "http://parts.igem.org/Part:BBa_K2100011">BBa_K2100011</a></td>
 
</tr>
 
</tr>
  
 +
<tr>
 +
<td> pEXPR hEF1a:mKate</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100012">BBa_K2100012</a></td>
 +
</tr>
 +
 +
<tr>
 +
<td> pEXPR hEF1a:tagBFP</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100013">BBa_K2100013</a></td>
 +
</tr>
  
 
<tr>
 
<tr>
−
<td> pEXPR TRE - BM3R1</td>
+
<td> pEXPR pTRE:BM3R1</td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100036">BBa_K2100036</a></td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100036">BBa_K2100036</a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td> pEXPR TRE - TALER14</td>
+
<td> pEXPR pTRE:TALER14</td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100037">BBa_K2100037</a></td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100037">BBa_K2100037</a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td> pEXPR TRE - TALER21</td>
+
<td> pEXPR pTRE:TALER21</td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100038">BBa_K2100038</a></td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100038">BBa_K2100038</a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td> pEXPR BM3R1 - eYFP</td>
+
<td> pEXPR pBM3R1:eYFP</td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100039">BBa_K2100039</a></td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100039">BBa_K2100039</a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td> pEXPR TALER14 - eYFP</td>
+
<td> pEXPR pTALER14:eYFP</td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100040">BBa_K2100040</a></td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100040">BBa_K2100040</a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
<td> pEXPR TALER21 - eYFP</td>
+
<td> pEXPR pTALER21:eYFP</td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100041">BBa_K2100041</a></td>
 
<td> <a href = "http://parts.igem.org/Part:BBa_K2100041">BBa_K2100041</a></td>
 
</tr>
 
</tr>
−
</table>
 
  
−
<br><br>
 
−
<br>
 
−
<br>
 
  
 +
<tr>
 +
<td> pEXPR pTALER14:mKate</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100045">BBa_K2100045</a></td>
 +
</tr>
  
  
−
<div class="column full_size">
+
<tr>
 +
<td> pEXPR pBM3R1:mKate</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100046">BBa_K2100046</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR pERE6:BM3R1</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100044">BBa_K2100044</a></td>
 +
</tr>
  
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
<tr>
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
<td> pEXPR pERE5:BM3R1</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100043">BBa_K2100043</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR pERE3:BM3R1</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100042">BBa_K2100042</a></td>
 +
</tr>
  
−
</div>
+
<tr>
 +
<td> pEXPR pERE6:TALER14</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100035">BBa_K2100035</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR pERE5:TALER14</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100034">BBa_K2100034</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR pERE3:TALER14</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100033">BBa_K2100033</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR pHybrid:eYFP</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100032">BBa_K2100032</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR pPRE4:eYFP</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100031">BBa_K2100031</a></td>
 +
</tr>
  
−
<div class="column half_size">
+
<tr>
−
<div class="highlight">
+
<td> pEXPR pPRE3:eYFP</td>
−
<h5>Note</h5>
+
<td> <a href = "http://parts.igem.org/Part:BBa_K2100030">BBa_K2100030</a></td>
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
</tr>
−
</div>
+
−
</div>
+
  
 +
<tr>
 +
<td> pEXPR pERE6:eYFP</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100029">BBa_K2100029</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR pERE5:eYFP</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100028">BBa_K2100028</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR pERE3:eYFP</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100027">BBa_K2100027</a></td>
 +
</tr>
  
−
<div class="column half_size">
+
<tr>
 +
<td> pEXPR pERE5:TP901</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100047">BBa_K2100047</a></td>
 +
</tr>
  
−
<h5>Adding parts to the registry</h5>
+
<tr>
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
<td> pEXPR pERE6:TP901</td>
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
+
<td> <a href = "http://parts.igem.org/Part:BBa_K2100052">BBa_K2100052</a></td>
−
</div>
+
</tr>
  
 +
<tr>
 +
<td> pEXPR EGSH: mKate</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100066">BBa_K2100066</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR EGSH: TP901</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100065">BBa_K2100065</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR hEF1a: VgEcR</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100067">BBa_K2100067</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR TRE: L7Ae</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100069">BBa_K2100069</a></td>
 +
</tr>
  
−
<div class="column half_size">
+
<tr>
 +
<td> pEXPR hEF1a: attB-flipped eYFP-attP</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100071">BBa_K2100071</a></td>
 +
</tr>
  
−
<h5>What information do I need to start putting my parts on the Registry?</h5>
+
<tr>
−
<p>The information needed to initially create a part on the Registry is:</p>
+
<td> pEXPR hEF1a - SV40: eYFP</td>
−
<ul>
+
<td> <a href = "http://parts.igem.org/Part:BBa_K2100072">BBa_K2100072</a></td>
−
<li>Part Name</li>
+
</tr>
−
<li>Part type</li>
+
−
<li>Creator</li>
+
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
  
−
<p>
+
<tr>
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
<td> pEXPR EGSH - 2kturn: TP901</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100074">BBa_K2100074</a></td>
 +
</tr>
  
−
</div>
+
<tr>
 +
<td> pEXPR EGSH - 2kturn: mKate</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100073">BBa_K2100073</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR EGSH - 4kturn: TP901</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_BBa_K2100077">BBa_K2100077</a></td>
 +
</tr>
  
−
<div class="column half_size">
+
<tr>
 +
<td> pEXPR EGSH - 4kturn: mKate</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100078">BBa_K2100078</a></td>
 +
</tr>
  
−
<h5>Inspiration</h5>
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<tr>
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
<td> pEXPR TRE: mKate</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100075">BBa_K2100075</a></td>
 +
</tr>
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
<tr>
−
<ul>
+
<td> pEXPR hEF1a: rtTA</td>
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
<td> <a href = "http://parts.igem.org/Part:BBa_BBa_K2100076">BBa_K2100076</a></td>
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
</tr>
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
−
</ul>
+
−
</div>
+
  
−
<div class="column full_size">
+
<tr>
−
<h5>Part Table </h5>
+
<td> pEXPR TRE: tagBFP</td>
−
<div class="highlight">
+
<td> <a href = "http://parts.igem.org/Part:BBa_K2100079">BBa_K2100079</a></td>
 +
</tr>
  
 +
<tr>
 +
<td> pEXPR hEF1a: Gal4 - VP16</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100080">BBa_K2100080</a></td>
 +
</tr>
  
−
</html>
 
−
<groupparts>iGEM2016 Example</groupparts>
 
−
<html>
 
−
</div>
 
−
</div>
 
  
 +
</table>
  
 +
<br>
 +
<h1 style="color:#FFFFFF; background-color:#F20253;; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: trebuchet MS"> Destination Vectors</h1>
 +
<br>
 +
<table>
 +
<tr>
 +
<td> pDEST mCherry GG</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100015">BBa_K2100015</a></td>
 +
</tr></table>
 +
</center>
 +
 +
 +
 +
<br><br>
 +
<br>
 +
<br>
 +
 +
<br>
 +
<h1 style="color:#FFFFFF; background-color:#F20253;; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: trebuchet MS"> miRNA Sensors</h1>
 +
<br>
 +
<center>
 +
<table>
 +
<tr>
 +
<td>Sensor 20A</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100051"> BBa_K2100051</a></td>
 +
</tr>
 +
<tr>
 +
<td> Sensor 142-5p</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100053">BBa_K2100053</a></td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td> Sensor 18A</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100054">BBa_K2100054</a></td>
 +
</tr>
 +
 +
<tr>
 +
<td> Sensor 181A</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100056">BBa_K2100056</a></td>
 +
</tr>
 +
 +
<tr>
 +
<td> Sensor 451A</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100057">BBa_K2100057</a></td>
 +
</tr>
 +
 +
<tr>
 +
<td> Sensor 34C</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100058">BBa_K2100058</a></td>
 +
</tr>
 +
 +
<tr>
 +
<td> Sensor 9-3p</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100059">BBa_K2100059</a></td>
 +
</tr>
 +
 +
<tr>
 +
<td> Sensor 21-5p</td>
 +
<td> <a href = "http://parts.igem.org/Part:BBa_K2100060">BBa_K2100060</a></td>
 +
</tr>
 +
 +
</table>
 +
<br>
 +
 +
 +
 +
<br><br>
 +
<br>
 +
<br>
  
  
 
</html>
 
</html>

Latest revision as of 02:55, 20 October 2016




Parts


Basic Parts - Entry Vectors


Part Name Part Number
pENTR hEF1a BBa_K2100003
pENTR pTRE BBa_K2100004
pENTR pERE3 BBa_K2100000
pENTR pERE5 BBa_K2100001
pENTR pERE6 BBa_K2100002
pENTR pPRE3 BBa_K2100008
pENTR pPRE4 BBa_K2100009
pENTR pHybrid BBa_K2100010
pENTR pEGSH BBa_K2100021
pENTR pBM3R1 BBa_K2100022
pENTR pTALER21 BBa_K2100023
pENTR pTALER14 BBa_K2100024
pENTR eYFP BBa_K2100005
pENTR mKate BBa_K2100006
pENTR tagBFP BBa_K2100007
pENTR mCherry BBa_K2100014
pENTR TALER14 BBa_K2100016
pENTR TALER21 BBa_K2100017
pENTR BM3R1 BBa_K2100018
pENTR TP901 BBa_K2100019
pENTR GAL4 - VP16 BBa_K2100020
pENTR rtTA BBa_K2100025
pENTR VgEcR BBa_K2100026
pENTR attB-flipped eYFP-attP BBa_K2100049
pENTR EGSH-2kturn BBa_K2100050
pENTR EGSH-4kturn BBa_K2100055
pENTR hEF1a-SV40 BBa_K2100061
pENTR L7Ae BBa_K2100068

Composite Parts - Expression Vectors


pEXPR hEF1a:eYFP BBa_K2100011
pEXPR hEF1a:mKate BBa_K2100012
pEXPR hEF1a:tagBFP BBa_K2100013
pEXPR pTRE:BM3R1 BBa_K2100036
pEXPR pTRE:TALER14 BBa_K2100037
pEXPR pTRE:TALER21 BBa_K2100038
pEXPR pBM3R1:eYFP BBa_K2100039
pEXPR pTALER14:eYFP BBa_K2100040
pEXPR pTALER21:eYFP BBa_K2100041
pEXPR pTALER14:mKate BBa_K2100045
pEXPR pBM3R1:mKate BBa_K2100046
pEXPR pERE6:BM3R1 BBa_K2100044
pEXPR pERE5:BM3R1 BBa_K2100043
pEXPR pERE3:BM3R1 BBa_K2100042
pEXPR pERE6:TALER14 BBa_K2100035
pEXPR pERE5:TALER14 BBa_K2100034
pEXPR pERE3:TALER14 BBa_K2100033
pEXPR pHybrid:eYFP BBa_K2100032
pEXPR pPRE4:eYFP BBa_K2100031
pEXPR pPRE3:eYFP BBa_K2100030
pEXPR pERE6:eYFP BBa_K2100029
pEXPR pERE5:eYFP BBa_K2100028
pEXPR pERE3:eYFP BBa_K2100027
pEXPR pERE5:TP901 BBa_K2100047
pEXPR pERE6:TP901 BBa_K2100052
pEXPR EGSH: mKate BBa_K2100066
pEXPR EGSH: TP901 BBa_K2100065
pEXPR hEF1a: VgEcR BBa_K2100067
pEXPR TRE: L7Ae BBa_K2100069
pEXPR hEF1a: attB-flipped eYFP-attP BBa_K2100071
pEXPR hEF1a - SV40: eYFP BBa_K2100072
pEXPR EGSH - 2kturn: TP901 BBa_K2100074
pEXPR EGSH - 2kturn: mKate BBa_K2100073
pEXPR EGSH - 4kturn: TP901 BBa_K2100077
pEXPR EGSH - 4kturn: mKate BBa_K2100078
pEXPR TRE: mKate BBa_K2100075
pEXPR hEF1a: rtTA BBa_K2100076
pEXPR TRE: tagBFP BBa_K2100079
pEXPR hEF1a: Gal4 - VP16 BBa_K2100080

Destination Vectors


pDEST mCherry GG BBa_K2100015





miRNA Sensors


Sensor 20A BBa_K2100051
Sensor 142-5p BBa_K2100053
Sensor 18A BBa_K2100054
Sensor 181A BBa_K2100056
Sensor 451A BBa_K2100057
Sensor 34C BBa_K2100058
Sensor 9-3p BBa_K2100059
Sensor 21-5p BBa_K2100060