Difference between revisions of "Team:MIT/Attributions"

 
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<h1 style="color:#f20253; text-align: center; font-size: 40px; line-height: 40px;">Attributions</h1>
  
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<p> We'd like to thank everyone who helped us this year to make iGEM not only possible but extremely fun! This has been a great experience and we couldn't have done it without help! </p>
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<u><b><h2>Promoter Engineering Subgroup Attributions</h2></b></u>
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<p>All estrogen and progesterone sensing constructs were designed and created by the members of our subgroup. Entry vectors for constitutive promoters (hEF1a), repressors (TAL14, BM3R1,TAL21), fluorescent genes (eYFP, BFP, etc.), and activators were provided by the Weiss Lab. All experiments on our promoters were performed by members of our subgroup and conceived by us the students. The student on our team who proposed the  minCMV architecture with upstream binding sites for the ER and PR was inspired by a class he had taken with Prof. Ron Weiss, Biological Circuit Engineering Laboratory, where he had done some basic promoter engineering work in yeast. The cells we sought to test our promoters with (MCF7, ISH, tHESC) were not available in our host lab (the Weiss Lab), so we initiated a collaboration with the Grifith Lab at MIT, affiliated with the Center for Gynepathology Research. Christi Cook and Linda Stockdale of the Griffith Lab provided us with the cells, as well as best practices for culturing and stimulating them. Prof. Linda Griffith and Prof. Ron Weiss advised us during lab meetings where we would present our data. Postdoc Brian Teague of the Weiss Lab was our direct supervisor in case we ran into any difficulties with our labwork; he also helped us initially optimize the transfection of tHESC using electroporation when we ran into difficulties with cationic lipid transfection.</p>
  
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<u><b><h2>miRNA Sensing Subgroup Attributions</h2></b></u>
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<p>All experiments were performed by members of our subgroup and conceived by us the students. All cloning was performed by members of our subgroup. The design, creation, and testing of pDest_mCherry was spearheaded by us after initial inspiration from Weiss Lab postdoc Brian Teague. Entry vectors for constitutive promoters (hEF1a), repressors (TAL14, BM3R1,TAL21), fluorescent genes (eYFP, BFP, etc.), and activators were provided by the Weiss Lab. miRNA sensors were provided by graduate student Jeremy Gam. tHESC cells were provided by a new collaboration we initiated with Prof. Griffith in the Weiss Lab. Postdoc Brian Teague of the Weiss Lab was our direct supervisor in case we ran into any difficulties with our labwork; he also helped us initially optimize the transfection of tHESC using electroporation when we ran into difficulties with cationic lipid transfection. </p>
  
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<u><b><h2>Recombinase Subgroup Attributions</h2></b></u>
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<p>All experiments were performed by members of our subgroup and conceived by us the students. All cloning was performed by members of our subgroup. Entry vectors for constitutive promoters (hEF1a), repressors (TAL14, BM3R1,TAL21), fluorescent genes (eYFP, BFP, etc.), and activators (VgEcr, rtTA) were provided by the Weiss Lab. Sequences for the recombinases with nuclear localization sequences were obtained through a collaboration with the Boston University Wet Lab iGEM team. Postdoc Brian Teague of the Weiss Lab was our direct supervisor in case we ran into any difficulties with our labwork. </p>
  
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<u><b><h2>The Weiss Lab - The Center for Synthetic Biology </h2></b></u>
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<h3><b>Brian Teague</b> </h3><p> Mentored us throughout the process of developing our own project and coached us in the lab skills we needed to implement it.</p>
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<h3><b>Jeremy Gam</b> </h3><p> Provided us with the miRNA sensors and advice on learning about and testing for different miRNA expression levels.</p>
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<h3><b>Ron Weiss</b> </h3><p> Provided us with resources from the lab and feedback on the direction of the project.</p>
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<b><u><h2>The Griffith Lab - Center for Gynepathology Research </h2></b></u>
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<h3><b>Linda Stockdale</b> </h3><p> Provided us with splits of tHESC, ISH, and MCF7 as well as estrogen and progesterone.</p>
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<h3><b>Christi Cook</b> </h3><p> Advising us on dilution protocols and best practice cell culture techniques.</p>
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<h3><b>Linda Griffith</b> </h3><p> Provided us with resources from the lab and feedback on the direction of the project.</p>
  
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. </p>
 
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<h5> Why is this page needed? </h5>
 
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
 
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
 
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<h5> What should this page have?</h5>
 
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<li>General Support</li>
 
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<li>Project support and advice</li>
 
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<li>Fundraising help and advice</li>
 
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<li>Lab support</li>
 
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<li>Difficult technique support</li>
 
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<li>Project advisor support</li>
 
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<li>Wiki support</li>
 
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<li>Presentation coaching</li>
 
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<li>Human Practices support</li>
 
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
 
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<h5> Can we base our project on a previous one? </h5>
 
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
 
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<h5>Inspiration</h5>
 
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<p>Take a look at what other teams have done:</p>
 
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
 
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
 
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
 
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
 
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<h5>Team training and Project start</h5>
 
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
 
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
 
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<li>When did you start this course?</li>
 
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<li>Are the syllabus and course materials freely available online?</li>
 
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<li>When did you start your brainstorming?</li>
 
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<li>When did you start in the lab?</li>
 
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<li>When did you start working on  your project?</li>
 
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Latest revision as of 03:01, 20 October 2016

Attributions


We'd like to thank everyone who helped us this year to make iGEM not only possible but extremely fun! This has been a great experience and we couldn't have done it without help!


Promoter Engineering Subgroup Attributions

All estrogen and progesterone sensing constructs were designed and created by the members of our subgroup. Entry vectors for constitutive promoters (hEF1a), repressors (TAL14, BM3R1,TAL21), fluorescent genes (eYFP, BFP, etc.), and activators were provided by the Weiss Lab. All experiments on our promoters were performed by members of our subgroup and conceived by us the students. The student on our team who proposed the minCMV architecture with upstream binding sites for the ER and PR was inspired by a class he had taken with Prof. Ron Weiss, Biological Circuit Engineering Laboratory, where he had done some basic promoter engineering work in yeast. The cells we sought to test our promoters with (MCF7, ISH, tHESC) were not available in our host lab (the Weiss Lab), so we initiated a collaboration with the Grifith Lab at MIT, affiliated with the Center for Gynepathology Research. Christi Cook and Linda Stockdale of the Griffith Lab provided us with the cells, as well as best practices for culturing and stimulating them. Prof. Linda Griffith and Prof. Ron Weiss advised us during lab meetings where we would present our data. Postdoc Brian Teague of the Weiss Lab was our direct supervisor in case we ran into any difficulties with our labwork; he also helped us initially optimize the transfection of tHESC using electroporation when we ran into difficulties with cationic lipid transfection.

miRNA Sensing Subgroup Attributions

All experiments were performed by members of our subgroup and conceived by us the students. All cloning was performed by members of our subgroup. The design, creation, and testing of pDest_mCherry was spearheaded by us after initial inspiration from Weiss Lab postdoc Brian Teague. Entry vectors for constitutive promoters (hEF1a), repressors (TAL14, BM3R1,TAL21), fluorescent genes (eYFP, BFP, etc.), and activators were provided by the Weiss Lab. miRNA sensors were provided by graduate student Jeremy Gam. tHESC cells were provided by a new collaboration we initiated with Prof. Griffith in the Weiss Lab. Postdoc Brian Teague of the Weiss Lab was our direct supervisor in case we ran into any difficulties with our labwork; he also helped us initially optimize the transfection of tHESC using electroporation when we ran into difficulties with cationic lipid transfection.

Recombinase Subgroup Attributions

All experiments were performed by members of our subgroup and conceived by us the students. All cloning was performed by members of our subgroup. Entry vectors for constitutive promoters (hEF1a), repressors (TAL14, BM3R1,TAL21), fluorescent genes (eYFP, BFP, etc.), and activators (VgEcr, rtTA) were provided by the Weiss Lab. Sequences for the recombinases with nuclear localization sequences were obtained through a collaboration with the Boston University Wet Lab iGEM team. Postdoc Brian Teague of the Weiss Lab was our direct supervisor in case we ran into any difficulties with our labwork.

The Weiss Lab - The Center for Synthetic Biology

Brian Teague

Mentored us throughout the process of developing our own project and coached us in the lab skills we needed to implement it.

Jeremy Gam

Provided us with the miRNA sensors and advice on learning about and testing for different miRNA expression levels.

Ron Weiss

Provided us with resources from the lab and feedback on the direction of the project.


The Griffith Lab - Center for Gynepathology Research

Linda Stockdale

Provided us with splits of tHESC, ISH, and MCF7 as well as estrogen and progesterone.

Christi Cook

Advising us on dilution protocols and best practice cell culture techniques.

Linda Griffith

Provided us with resources from the lab and feedback on the direction of the project.