Difference between revisions of "Team:MIT/Mammalian synthetic biology kit"
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Revision as of 05:27, 8 October 2016
With the development of efficacious gene editing tools and improved practices, mammalian synthetic biology grows more prevalent. While our project is one example of how synthetic biology can be used to diagnose diseases, we hope that more people are able to build off our work, and the work of others to apply synthetic biology to mammalian cells..
Hoping to see what amazing work can come from future teams and scientists, we have put together a toolbox of parts to make it easier to start mammalian work.
Our kit is meant to be used with Gateway cloning reactions. In it we provide a pDEST destination vector that has a screening tool for choosing successful transformations. Along with this, we include several pENTR vectors, with promoters and useful genes that can be combined and put into eColi to grow up before transfection into mammalian systems
Explain how the gateways work or no?
needs to be linked to the protocol/parts page, but right now linked to what may be a throwaway
hef1a is a constitutive promoter. Connected to various fluorescence, these constructs can be used for transfection markers
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