Difference between revisions of "Team:MIT/Experiments/miRNA/more experiments"
| Line 12: | Line 12: | ||
<h2 style="text-decoration:underline"> Purpose</h2> | <h2 style="text-decoration:underline"> Purpose</h2> | ||
<p> We want to understand the sensitivity of our miRNA target site constructs. </p> | <p> We want to understand the sensitivity of our miRNA target site constructs. </p> | ||
| + | <br> | ||
<h2 style="text-decoration:underline”> Set Up </h2> | <h2 style="text-decoration:underline”> Set Up </h2> | ||
| − | <p > We chose one miRNA target site, 451a, and designed and ordered siRNA complementary to the target site. We co-transfected tert-immortalized human endometrial stromal cells with our miRNA sensors and varying concentrations of siRNA then measured the red fluorescence output to gauge repression. </p> | + | <p> We chose one miRNA target site, 451a, and designed and ordered siRNA complementary to the target site. We co-transfected tert-immortalized human endometrial stromal cells with our miRNA sensors and varying concentrations of siRNA then measured the red fluorescence output to gauge repression. </p> |
<br> | <br> | ||
<img src = "https://static.igem.org/mediawiki/2016/6/6d/T--MIT--sensor.PNG" style = 'padding: 5px'; width: 400px; height = 400px; float: right; border:5px;'> | <img src = "https://static.igem.org/mediawiki/2016/6/6d/T--MIT--sensor.PNG" style = 'padding: 5px'; width: 400px; height = 400px; float: right; border:5px;'> | ||
Revision as of 18:45, 16 October 2016
Sensitivity of miRNA Target Sites
Purpose
We want to understand the sensitivity of our miRNA target site constructs.
Results
When increasing siRNA-451a 0 to 1 nM, there is a 10 fold repression of red fluorescence. Saturation occurs at approximately 10 nM. More experiments would need to be done to understand the responsiveness between 0 to 1 nM.
miRNA Profile of tHESC
