Revision as of 04:46, 8 October 2016

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L7Ae - kink turn Repressing System

System mechanism

L7Ae is an RNA binding protein, and a K turn is an RNA sequence that L7Ae recognizes and binds. The binding activity of L7Ae will prevent the ribosome machinery from performing translation. Using the RNA level control with the inducible promoter, we hope to tune the basal expression of the recombinase to the level that will sense the difference between the levels of repressors and miRNA in the healthy and diseased states of endometrial cells. We will first try this experiment in HEK293, and then we will move on to MCF-7 and tHESC.

Experiment

1. Set up

2. Results

Testing the 2x k-turn L7Ae system with varied L7Ae expression level

Testing the effect of varying k-turn sequences