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| | <img src = "https://static.igem.org/mediawiki/2016/9/92/T--MIT--hef1mkate.png" style ="width: 318px: height = 127px;"> | | <img src = "https://static.igem.org/mediawiki/2016/9/92/T--MIT--hef1mkate.png" style ="width: 318px: height = 127px;"> |
| | <img src = "https://static.igem.org/mediawiki/2016/b/b4/T--MIT--hef1aeyfp.png" style ="width: 318px: height = 127px;"> | | <img src = "https://static.igem.org/mediawiki/2016/b/b4/T--MIT--hef1aeyfp.png" style ="width: 318px: height = 127px;"> |
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| − | <p>these will be changed to the various pENTRs that can we include in said kit </p>
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| − | <a href="https://2016.igem.org/Team:MIT/Medals"><img src="https://static.igem.org/mediawiki/2016/6/6e/T--MIT--MedalsButton2.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Judge"><img src="https://static.igem.org/mediawiki/2016/5/57/T--MIT--JudgingButton.svg" style="width:225px;height=225px;"></a><a href="https://2016.igem.org/Team:MIT/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/8/86/T--MIT--HPButton.svg" / style="width:225px;height=225px;"></a><br>
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| − | <h5>Note</h5>
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| − | <p>You must fill out this page in order to be considered for all <a href="https://2016.igem.org/Judging/Awards">awards</a> for Human Practices:</p>
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| − | <li>Human Practices silver medal criterion</li>
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| − | <li>Human Practices gold medal criterion</li>
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| − | <li>Best Integrated Human Practices award</li>
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| − | <li>Best Education and Public Engagement award</li>
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| − | <h5>Some Human Practices topic areas </h5>
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| − | <li>Philosophy</li>
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| − | <li>Education</li>
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| − | <li>Product Design</li>
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| − | <li>Scale-Up and Deployment Issues</li>
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| − | <li>Security</li>
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| − | <h5>What should we write about on this page?</h5>
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| − | <p>On this page, you should write about the Human Practices topics you considered in your project, and document any special activities you did (such as visiting experts, talking to lawmakers, or doing public engagement).</p>
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| − | <h5>Inspiration</h5>
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| − | <p>this is so I know how to add a link if I so desire!:</p>
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| − | <li><a href="https://2014.igem.org/Team:Dundee/policypractice/experts">2014 Dundee </a></li>
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Revision as of 05:28, 8 October 2016
With the development of efficacious gene editing tools and improved practices, mammalian synthetic biology grows more prevalent. While our project is one example of how synthetic biology can be used to diagnose diseases, we hope that more people are able to build off our work, and the work of others to apply synthetic biology to mammalian cells..
Hoping to see what amazing work can come from future teams and scientists, we have put together a toolbox of parts to make it easier to start mammalian work.
Our kit is meant to be used with Gateway cloning reactions. In it we provide a pDEST destination vector that has a screening tool for choosing successful transformations. Along with this, we include several pENTR vectors, with promoters and useful genes that can be combined and put into eColi to grow up before transfection into mammalian systems
Explain how the gateways work or no?
needs to be linked to the protocol/parts page, but right now linked to what may be a throwaway
hef1a is a constitutive promoter. Connected to various fluorescence, these constructs can be used for transfection markers
