Difference between revisions of "Team:MIT/Experiments/miRNA"
| Line 36: | Line 36: | ||
<h1 style="color:#000000; background-color:#FF9733; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS"> How can our circuit sense miRNA? </h1> | <h1 style="color:#000000; background-color:#FF9733; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS"> How can our circuit sense miRNA? </h1> | ||
| + | <img src = "https://static.igem.org/mediawiki/2016/c/c2/T--MIT--miRNA_ts_setup.PNG" style = 'padding: 5px 10px 5x 10px; width: 400px; height = 400px; float: right; border:10px;'> | ||
<p style="font-family: Verdana;"> | <p style="font-family: Verdana;"> | ||
| Line 45: | Line 46: | ||
<h1 style="color:#000000; background-color:#FF9733; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS"> Are our target sites sensitive to miRNA levels? </h1> | <h1 style="color:#000000; background-color:#FF9733; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS"> Are our target sites sensitive to miRNA levels? </h1> | ||
| + | |||
<p style="font-family: Verdana;"> | <p style="font-family: Verdana;"> | ||
Revision as of 23:39, 9 October 2016
What can we learn from miRNA?
miRNA are small units of RNA that play a regular role in gene regulation. miRNA binds and cleaves messenger RNA, inhibiting gene expression. There are hundreds of miRNA, and their profile and activity vary between different cell types.
This makes it is possible to tell the difference between cells from the uterus and liver based on miRNA profiles. Furthermore, cells from the uterus of women with endometriosis have been found to have dysregulated miRNA in comparison to cells from the uterus of women who do not have endometriosis().
How can our circuit sense miRNA?
(Insert Figure right ) Our circuit utilizes the natural function of miRNA to produce a differential output depending on whether the cell has dysregulated miRNA. This is achieved by attaching 4 tandem sites complementary to the affected miRNA following a gene of interest.
Are our target sites sensitive to miRNA levels?
These 4 tandem sites for miRNA binding were tested by coding for them distal to the gene for red fluorescent protein. We were able to see a ten fold repression upon increasing the concentration of siRNA from 0 to 1 nM. Saturation appeared at about 10 nM.
(graph)
More information on testing target site sensitivity.
