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Revision as of 18:42, 16 October 2016

Description of Experiments

Sensitivity of miRNA Target Sites


Purpose

We want to understand the sensitivity of our miRNA target site constructs.

Results



When increasing siRNA-451a 0 to 1 nM, there is a 10 fold repression of red fluorescence. Saturation occurs at approximately 10 nM. More experiments would need to be done to understand the responsiveness between 0 to 1 nM.



miRNA Profile of tHESC