Team:MIT/L7AeRepressingSystem

• BACKGROUND
• EXPERIMENTS
  Synthetic Promoters microRNA Recombinase Proof of Concept Collaborations
• FUTURE WORK
  Circuit Design Clinical Applications
• HUMAN PRACTICES
  Parts Toolbox Accessible Protocols Integrated Human Practices Public Outreach Interlab Study
• NOTEBOOK
  Lab Notebook Parts Mammalian Parts Collection Attributions Safety
• AWARDS
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• TEAM

L7Ae - kink turn Repressing System

System mechanism

L7Ae is an RNA binding protein, and a K turn is an RNA sequence that L7Ae recognizes and binds. The binding activity of L7Ae will prevent the ribosome machinery from performing translation. Using the RNA level control with the inducible promoter, we hope to tune the basal expression of the recombinase to the level that will sense the difference between the levels of repressors and miRNA in the healthy and diseased states of endometrial cells. We will first try this experiment in HEK293, and then we will move on to MCF-7 and tHESC.

Experiment

1. Set up

Read more about building kturn constructs here.