Team:MIT/Experiments/miRNA/more experiments

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  Synthetic Promoters microRNA Recombinase Proof of Concept Collaborations
• FUTURE WORK
  Circuit Design Clinical Applications
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General Experimental Set-Up and Data Analysis

miRNA Sensor

Read about here

Transient Transfection

Flow Cytometry Analysis

Sensitivity of miRNA Target Sites

Purpose

We want to understand the sensitivity of our miRNA target site constructs.

Set Up

We chose one miRNA target site, 451a, and designed and ordered siRNA complementary to the target site. We co-transfected tert-immortalized human endometrial stromal cells with our miRNA sensors and varying concentrations of siRNA then measured the red fluorescence output to gauge repression.

Results

When increasing siRNA-451a 0 to 1 nM, there is a 10 fold repression of red fluorescence. Saturation occurs at approximately 10 nM. More experiments would need to be done to understand the responsiveness between 0 to 1 nM.

miRNA Profile of tHESC

Results