Team:Stanford-Brown/SB16 Notebooks AptamerPurification
Aptamer Purification (DNAzyme, PQQ, SELEX)
Made with Benchling
Project: iGEM 2016
Authors: Michael Becich, Amy Weissenbach, Charles Gleason, Julia Gross
Dates: 2016-08-29 to 2016-08-31
Monday, 8/29
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Made B&W Buffer
Tuesday, 8/30
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Made 1M Lysine-HCl Stock
Wednesday, 8/31
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Made 2x Binding Buffer (100mM HEPES, 200mM KCl, 50mM MgCl2, 10mM CaCl2)
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Made 1M CuSO4 stock
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Washed Magnetic Streptavidin Dynabeads
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Immobilized Biotinylated Oligonucleotides on beads
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Incubated overnight
