3I0A]) together with the bound siderophor enterobactin and an iron atom.

We met the iGEM Team from Sheffield University at the European Experience in Paris where we presented our projects to each other. When they told us that they are using the human Lipocalin 2 (Lcn2) in order to detect the siderophors of pathogenic bacteria, we came up with the idea that we could provide the team with highly pure Lcn2 protein. This was an useful collaboration as the main PI of the Munich iGEM team 2016, Prof. Dr. Arne Skerra, is one of the leading experts in the field of lipocalins^[1]. For this reason the expression constructs and the protocols for the production of this recombinant protein are well established in the lab we are affiliated to. The main challenge for the production of the recombinant protein was that the Sheffield iGEM team needed the apo-form of the protein without the bacterial siderophor as they want to scavenge siderophors from samples and analyse the protein afterwards for their concent of siderophors. The challenge here was to produce the recombinant Protein in E. coli in a way that it is no occupied by the siderophor that is produced by most E. coli strains.

Collaboration with iGEM FAU Erlangen:
A) Production and purification strategy, B) Size-exclusion chromatography, C) Filtration, D) Tube readied for shipment, E) Yield after purification

The iGEM team from FAU Erlangen is working on the development of a biological photovoltaic solar cell for which they want to use fluorescent proteins to harvest the photons. We saw their presentation at the iGEM Germany meet-up in Marburg and as we are using eGFP anyway for your own project as a purified recombinant protein we proposed to provide them with some eGFP protein for their experiments. Luckily, at the "Biotechnikum" of the TU Munich there is a internship for bioprocess engineering students in every spring in which they produce a large badge of eGFP in E. coli at a bioprocess scale of several hundred liters of culture medium. For our project we were provided with ~ 50 gram of eGFP that we subsequently purified by ammonium-sulfate precipitation an immobilized metal affinity chromatography (IMAC) and dialyzed the protein finally against PBS. At the beginning of October we provided 1.4 g of purified eGFP to the FAU Erlangen iGEM team.

Collaboration with iGEM FAU Erlangen:
A) , B)

In order to get some first feedback for our bioprinter, we agreed with the iGEM Team from the TU Darmstadt that they become our beta-tester for the syringe pump we constructed for our bioprinter as well as for the whole synbio community. Our syringe pump has proven to work well in our lab and it can be built with less than 50 USD of investment if a normal 3D printer is available. The beta-testing was that they only got the files for the 3D printing as well as all the parts (such as a stepper motor, few screws, some end-stop switches and the necessary wiring.

Here is their feedback after they assembled our syringe pump:

"The pump is neat and stable. The idea of using buttons as end stops is in principle good. You took the necessary holes into account and we were able to assemble it according to the instructions. The click system for the syringe was well made and worked straightaway. The linear bearing could be used right away as well.

We utilized a homemade 3D printer on highest gear for printing the parts. As you might know this leads to relatively many "aberrations" and errors in the prints. They made the assembly difficult and we had to post process several of them.

Your construction is designed quite closed, which renders it difficult for any passionate tinkerer to make adaptations after the printing. For instance we noted that you had sent us a printed clutch though the stepper motor was not shortened and did not fit. Therefor we drilled a hole in the front, enlarged the clutch and clued the parts together. In general there are different stepper motors in use in the DIY community, hence we suggest to concept a more general clutch to ease this problem. Furthermore the suspension device for the syringe could be improved, though the varying design of DIY printers makes finding a "good" solution rather difficult."

Munich

Following the invitation by the teams from iGEM IONIS and Evry we packed our suitcases and went off.

Paris

Montmartre

Team stand

And after a little climbing tour to Montmartre and maybe some Super-Last-Minute poster printing ;), we settled for the night in our shady "lodging" to be ready to showcase our project for the first time publicly. Early in morning we headed to the event location. First objective: COFFEE,
which ended to be a great challenge than expected. Don't bring a knife (instant coffee) to a gun fight (horde of thirsty, tired students) :D

Fire performance

But after breakfast was aquired things started to get interesting. Dozens of awesome projects from all over Europe and even the UK! It was great to see all those ideas and of course to be able to show our bioprinting project to the world. We also got some helpful tips and hints. After the poster sessions was over and food was ingested, some interesting panels on ethics and entrepreneurship in synthetic biology followed before it was time for Randy to drop some final Brexit jokes and conclude the scientific section of the event.

Party

The evening promised to be a great dance party in "downtown paris" including a live stream of the quarterfinal match of the European Championship between Italy and of course Germany. After this sweat-braking super-tight overly nerve-wrecking game was finally over, with the better end for us :), we hit the dance floor down in the underground car park. The midnight surprise, breathtaking fire-breather, which for some reason was interrupted by the security guard, I wonder why ;). With this awesome party night marking the end of the [http://www.facebook.com/events/458143747717965/ European Experience] we used the following Sunday to enjoy a wonderful french breakfast and explore the tourist sites of the "City of Love" before it was time to board the plane back to the lab. What a great event!

Just one month after the European Experience it was time for the next big meetup, the German meetup in the historic city Marburg with its winding streets and steep and tiny alleys.

So instead of boarding a plane we loaded the car and went on a little road trip of 450 km through wind and rain. Luckily weather got better and better the closer we came. Just after arriving at the camp site and setting everything up, the first official point of the program was already in its beginnings: bar tour, yeah! A long and noisy night followed ;) The next morning the teams from literally all over Germany and extended-Germany, also known as Denmark, assembled at main event building and grabbed some awesome breakfast, including appropriate amounts of coffee, before be welcomed by our great hosts from [http://www.facebook.com/igemmarburg iGEM Marburg]. After some introduction speeches by the sponsors of the event the teams took the stage and presented their projects. As some time had passed since the Paris event we got to see how the ideas developed and of course their were also some we had not seen before. Really awesome! And after grabbing lunch it was our turn to show what we have been and would be doing in lab. The afternoon was dedicated to physical activity of a special kind, Bubble Soccer. Probably the most hilarious game ever, though it will drain all your energy. Luckily our hosts had anticipated this and provided us with a tasty BBQ. Though everyone was a bit tired, the day was far from over as there was the final party of the weekend. The next morning came way to quickly but luckily it was a beautiful sunny morning. We had the chance to hike to the castle and enjoy the amazing view from the top before it was again time to say Goodbye for a while and hit the road back to the lab. Thanks again Marburg for having us, it was a pleasure!

This years team of Highschool students from Ankara came in touch with us and asked for help for their clever idea of translating the standard iGEM lab protocols into different languages. This will hopefully help future teams from all around the globe to better understand them and apply them in the lab without being required to understand English perfectly. Especially other future Highschool teams should profit from this.
We are happy to be part such a great project and make iGEM even more international.

A rose from Munich

As part of their project dealing with the most prevalent mutation in the CFTR protein, responsible for Cystic Fibrosis, the team from Tel Hai Academic college dedicated themselves to raise awareness for the life-shortening genetic condition. The “65 Roses” story itself dates back to 1965 when an observant 4-year-old, hearing the name of his disease for the first time, pronounced cystic fibrosis as "65 Roses." Nowadays, “65 Roses” is a term often used by young children with cystic fibrosis to pronounce the name of their disease. We are very happy to be a part of their noble efforts and dedication for this project.

Due to the history of interesting projects from Munich it did not take long for the first Biobricks request coming from all over the world.

Vive le France!

The first inquiry came from our cave saving colleagues from Toulouse were in need for a [http://parts.igem.org/Part:BBa_K823026 BioBrick for their Bacillus subtilis] originally developed by the team from LMU in 2012. Go save those caves! ;)

The far east

Not long after a more distant call reached us all the way from - 2016 XMU-China iGEM team. They required components from the biosensor part from the LMU team 2014 in order to detect and kill Staphylococcus aureus.

Our dutch neighbours

At last but not least the team from Groningen came in touch with us regarding yet another Biobrick for Bacillus subtilis from LMU 2012.

In order to properly display our project we decided to use the hightech 3D animation software [http://www.autodesk.de/products/maya/overview Maya by Autodesk]. But as we think this is an awesome way to show people what you have been doing all summer long we thought we give you a short introduction video on HowTo do it. We hope this helps you to get started and as we really want to see more of this animations we also added our "cell" template file for you to download:

File:Muc16 CellTemplate.pdf

1. ↑ Schiefner, A., & Skerra, A. (2015). The menagerie of human lipocalins: a natural protein scaffold for molecular recognition of physiological compounds. Accounts of chemical research, 48(4), 976-985.