Team:LMU-TUM Munich/Basic Part

Basic Parts we improved

• Tet-Operator: [http://parts.igem.org/Part:BBa_I739001 BBa_I739001] was improved by confirming functionality, adding a Tet-Operator together with an OmpA secretion signal yielding the part [http://parts.igem.org/Part:BBa_K2170141 BBa_K2170141] which can be used by teams for the SEC-dependent secretion in the bacterial periplasm.

• The collection of eukaryotic signal peptides in RFC[25] was expanded significantly by identifying non-working signal peptides ([http://parts.igem.org/Part:BBa_I712009 BBa_I712009], as not in RFC[25]), characterizing working signal peptides ([http://parts.igem.org/Part:BBa_K157001 BBa_K157001]) and designing, constructing and testing two additional signal peptides with significantly higher functionality ([http://parts.igem.org/Part:BBa_K2170214 BBa_K2170214] & [http://parts.igem.org/Part:BBa_K2170215 BBa_K2170215])

<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>