Featured Composite Part:

TetR-TetO-OmpA ([http://parts.igem.org/Part:BBa_K2170141 BBa_K2170141]):

The experimental controle over the gene expression of a specific gene can be essential when you are working with microorganisms. This is the reason why we tested the BioBrick [http://parts.igem.org/Part:BBa_I739001 BBa_I739001] of ETHZ 2007 for functionality and added a tetracycline operator as well as an OmpA signal peptide, which imparts the SEC-dependent secretion of the downstream protein. The construct was tested using a testing device [http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170053 BBa_K2170053] whose secretion efficiency could be tested by NanoGlo assay.

Biotinylated receptor with BAP ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170000 BBa_K2170000])

Our BioBrick [http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170000 BBa_K2170000] contains an eucaryotic receptor construct with an biotin acceptor peptide, which, after expression in an eucaryotic cell, imparts the binding of streptavidin on the cell surface. This constucts makes it possible to link cells with a biotin binding protein and by this makes the printing of tissue without scaffold even possible.

Biotin binding receptor with eMA ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170001 BBa_K2170001])

Our BioBrick [http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170001 BBa_K2170001] contains an eucaryotic receptor construct with enhanced monomeric avidin, which, after expression in an eucaryotic cell, imparts the binding of biotin on the cell surface. This constucts makes it possible to link cells over a biotinylated protein linker and by this makes the printing of tissue without scaffold even possible.

Biotin binding receptor with scAvidin ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002])

Our BioBrick [http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002] contain an eucaryotic biotin binding receptor with the tetrameric biotin binding protein single chain avidin. As it's sibling BioBrick [http://parts.igem.org/wiki/index.php?title=Part:BBa_K2170001 BBa_K2170001] it imparts the polymerization of cells, after transfection, because of the ability to bind biotinylated protein linkers.

Composite Parts we improved:

GMK_TK90 "Kill-Switch": [http://parts.igem.org/Part:BBa_K404113 BBa_K404113] & [http://parts.igem.org/Part:BBa_K782063 BBa_K782063] were improved as both parts were wrong as they both contained a forbidden PstI restriction site that made it impossible to ligate BioBricks downstream of the BioBrick. A corrected BioBrick was provided ([http://parts.igem.org/Part:BBa_K2170060 BBa_K2170060])

<groupparts>iGEM2016 LMU-TUM_Munich</groupparts>